Macrophage-stimulating protein induces proliferation and migration of murine keratinocytes

Ming Hai Wang, Andrzej A. Dlugosz, Yi Sun, Toshio Suda, Alison Skeel, Edward J. Leonard

Research output: Contribution to journalArticle

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Abstract

Macrophage stimulating protein (MSP) is a chemotactic factor for murine peritoneal macrophages. The receptor for human MSP was recently identified as the ron gene product, a transmembrane protein tyrosine kinase cloned from a human keratinocyte cDNA library. Here we report that MSP induced proliferation of murine primary keratinocytes and established keratinocyte cell lines in a concentration-dependent manner. The growth efficacy of MSP was comparable to that of epidermal growth factor and keratinocyte growth factor. In three of four cell lines tested in a chemotaxis chamber, MSP also stimulated migration of keratinocytes on a collagen type IV substratum. The action of MSP was mediated by specific binding of MSP to the STK gene product, a murine homologue of the RON MSP receptor. Binding of MSP to keratinocyte STK induced phosphorylation of the 150 kDa STK β chain. Herbimycin A, a protein tyrosine kinase inhibitor, blocked MSP-mediated phosphorylation of the STK receptor as well as proliferation of keratinocytes, suggesting the importance of tyrosine kinase activity for transduction of the message delivered by MSP. Previously, the only known target cell for MSP was the resident peritoneal macrophage. These studies establish the keratinocyte as a new target cell for MSP. The action of MSP on keratinocytes may have implications for tissue repair, wound healing, and tumor growth.

Original languageEnglish
Pages (from-to)39-46
Number of pages8
JournalExperimental Cell Research
Volume226
Issue number1
DOIs
Publication statusPublished - 1996 Jul 10

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Keratinocytes
Protein-Tyrosine Kinases
Peritoneal Macrophages
macrophage stimulating protein
Phosphorylation
Fibroblast Growth Factor 7
Cell Line
Collagen Type IV
Chemotactic Factors
Chemotaxis
Protein Kinase Inhibitors
Growth
Gene Library
Epidermal Growth Factor
Wound Healing
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Wang, M. H., Dlugosz, A. A., Sun, Y., Suda, T., Skeel, A., & Leonard, E. J. (1996). Macrophage-stimulating protein induces proliferation and migration of murine keratinocytes. Experimental Cell Research, 226(1), 39-46. https://doi.org/10.1006/excr.1996.0200

Macrophage-stimulating protein induces proliferation and migration of murine keratinocytes. / Wang, Ming Hai; Dlugosz, Andrzej A.; Sun, Yi; Suda, Toshio; Skeel, Alison; Leonard, Edward J.

In: Experimental Cell Research, Vol. 226, No. 1, 10.07.1996, p. 39-46.

Research output: Contribution to journalArticle

Wang, MH, Dlugosz, AA, Sun, Y, Suda, T, Skeel, A & Leonard, EJ 1996, 'Macrophage-stimulating protein induces proliferation and migration of murine keratinocytes', Experimental Cell Research, vol. 226, no. 1, pp. 39-46. https://doi.org/10.1006/excr.1996.0200
Wang, Ming Hai ; Dlugosz, Andrzej A. ; Sun, Yi ; Suda, Toshio ; Skeel, Alison ; Leonard, Edward J. / Macrophage-stimulating protein induces proliferation and migration of murine keratinocytes. In: Experimental Cell Research. 1996 ; Vol. 226, No. 1. pp. 39-46.
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