Macrophages fine-tune pupil shape during development

Moe Takahashi, Mika Misaki, Shinsuke Shibata, Takahito Iga, Tomoko Shindo, Ikue Tai-Nagara, Ayako Hirata, Marina Ogawa, Takeshi Miyamoto, Taneaki Nakagawa, Masatsugu Ema, Yusuke Ichiyama, David T. Shima, Katsuto Hozumi, Satoshi Nishimura, Yoshiaki Kubota

Research output: Contribution to journalArticlepeer-review

Abstract

Tissue macrophages, which are ubiquitously present innate immune cells, play versatile roles in development and organogenesis. During development, macrophages prune transient or unnecessary synapses in neuronal development, and prune blood vessels in vascular development, facilitating appropriate tissue remodeling. In the present study, we identified that macrophages contributed to the development of pupillary morphology. Csf1op/op mutant mice, in which ocular macrophages are nearly absent, exhibited abnormal pupillary edges, with abnormal protrusions of excess iris tissue into the pupillary space. Macrophages located near the pupillary edge engulfed pigmented debris, which likely consisted of unnecessary iris protrusions that emerge during smoothening of the pupillary edge. Indeed, pupillary edge macrophages phenotypically possessed some features of M2 macrophages, consistent with robust tissue engulfment and remodeling activities. Interestingly, protruding irises in Csf1op/op mice were only detected in gaps between regressing blood vessels. Taken together, our findings uncovered a new role for ocular macrophages, demonstrating that this cell population is important for iris pruning during development.

Original languageEnglish
Pages (from-to)137-144
Number of pages8
JournalDevelopmental Biology
Volume464
Issue number2
DOIs
Publication statusPublished - 2020 Aug 15

Keywords

  • Angiogenesis
  • Dll4
  • Macrophage
  • Pupillary membrane
  • Retina
  • VEGF

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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