Mad2 inhibits the mitotic kinesin MKlp2

Sang Hyun Lee, Frank McCormick, Hideyuki Saya

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We identified the mitotic kinesin-like protein 2 (MKlp2), a kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis, as a target of the mitotic checkpoint protein Mad2. MKlp2 possesses a consensus Mad2-binding motif required for Mad2 binding. Mad2 prevents MKlp2 from loading onto the mitotic spindle, a prerequisite step for its function as a mitotic kinesin. Furthermore, Mad2 inhibits the ability of MKlp2 to relocate the CPC from centromeres, an essential step to promote cytokinesis. An MKlp2 mutant that is refractory to Mad2-mediated inhibition prematurely translocates to the mitotic spindle and mislocalizes the CPC component Aurora B from the midbody of dividing cells. This correlates with an increased incidence of cytokinesis failure. Together, these findings reveal that MKlp2 is a novel mitotic target of Mad2 necessary for proper mitotic progression and cytokinesis.

Original languageEnglish
Pages (from-to)1069-1077
Number of pages9
JournalJournal of Cell Biology
Volume191
Issue number6
DOIs
Publication statusPublished - 2010 Dec 13

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Kinesin
Cytokinesis
Proteins
Spindle Apparatus
Chromosomes
Mad2 Proteins
M Phase Cell Cycle Checkpoints
Centromere
Mutant Proteins
Incidence

ASJC Scopus subject areas

  • Cell Biology

Cite this

Mad2 inhibits the mitotic kinesin MKlp2. / Lee, Sang Hyun; McCormick, Frank; Saya, Hideyuki.

In: Journal of Cell Biology, Vol. 191, No. 6, 13.12.2010, p. 1069-1077.

Research output: Contribution to journalArticle

Lee, Sang Hyun ; McCormick, Frank ; Saya, Hideyuki. / Mad2 inhibits the mitotic kinesin MKlp2. In: Journal of Cell Biology. 2010 ; Vol. 191, No. 6. pp. 1069-1077.
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