Magnetically promoted rapid immunoreactions using functionalized fluorescent magnetic beads: A proof of principle

Satoshi Sakamoto, Kenshi Omagari, Yoshinori Kita, Yusuke Mochizuki, Yasuyuki Naito, Shintaro Kawata, Sachiko Matsuda, Osamu Itano, Hiromitsu Jinno, Hiroya Takeuchi, Yuki Yamaguchi, Yuko Kitagawa, Hiroshi Handa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

BACKGROUND: Accurate detection and monitoring of disease-related biomarkers is important in understanding pathophysiology. We devised a rapid immunoreaction system that uses submicrometer polymercoated fluorescent ferrite (FF) beads containing both ferrites (magnetic iron oxide) and fluorescent europium complexes. METHODS: FF beads were prepared by encapsulation of hydrophobic europium complexes into the polymer layers of affinity magnetic beads using organic solvent. A sandwich immunoassay using magnetic collection of antibody-coated FF beads to a specific place was performed. Brain natriuretic peptide and prostate-specific antigen were selected as target detection antigens to demonstrate the feasibility of this approach. An immunohistochemical staining using magnetic collection of antibody-coated FF beads onto carcinoma cell samples was also performed. RESULTS: The sandwich immunoassays, taking advantage of the magnetic collection of antibody-coated FF beads, detected target antigens within 5 min of sample addition. Without magnetic collection, the sandwich immunoassay using antibody-coated FF beads required long times, similar to conventional immunoassays. Using the magnetic collection of antibody-coated FF beads, immunohistochemical staining enabled discrimination of carcinoma cells within 20 min. CONCLUSIONS: This proof of principle system demonstrates that immunoreactions involving the magnetic collection of antibody-coated FF beads allow acceleration of the antigen-antibody reaction. The simple magnetic collection of antibody-coated FF beads to a specific space enables rapid detection of disease-related biomarkers and identification of carcinoma cells.

Original languageEnglish
Pages (from-to)610-620
Number of pages11
JournalClinical Chemistry
Volume60
Issue number4
DOIs
Publication statusPublished - 2014 Apr

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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    Sakamoto, S., Omagari, K., Kita, Y., Mochizuki, Y., Naito, Y., Kawata, S., Matsuda, S., Itano, O., Jinno, H., Takeuchi, H., Yamaguchi, Y., Kitagawa, Y., & Handa, H. (2014). Magnetically promoted rapid immunoreactions using functionalized fluorescent magnetic beads: A proof of principle. Clinical Chemistry, 60(4), 610-620. https://doi.org/10.1373/clinchem.2013.211433