Maintenance of HCT116 colon cancer cell line conforms to a stochastic model but not a cancer stem cell model

Kazuharu Kai, Osamu Nagano, Eiji Sugihara, Yoshimi Arima, Oltea Sampetrean, Takatsugu Ishimoto, Masaya Nakanishi, Naoto T. Ueno, Hirotaka Iwase, Hideyuki Saya

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The cancer stem cell (CSC) model, in which a small population of cells within a tumor possesses the ability to self-renew and reconstitute the phenotype of primary tumor, has gained wide acceptance based on evidence over the past decade. It has also been reported that cancer cell lines contain a CSC subpopulation. However, phenotypic differences between CSCs and non-CSCs in cancer cell lines are not better defined than in primary tumors. Furthermore, some cell lines do not have a CSC population, revealed as a side population and expression of CD133. Thus, the identification of CSCs in cancer cell lines remains elusive. Here, we investigated the CSC hierarchy within HCT116 colon cancer cells, which do not have a CD133-positive subpopulation. We examined the expression of alternative CSC markers epithelial specific antigen (ESA) and CD44 in floating-sphere-derived cells, which are known to be the cells of enriching CSCs. Sphere-derived HCT116 cells exhibited heterogeneous expression of ESA and CD44. The two major subpopulations of HCT116 sphere cells (ESAlowCD44-/low and ESAhighCD44high) exhibited a biological/proliferative hierarchy of sphere-forming and soft agar colony-forming activity. However, there was no difference between the two subpopulations in the incidence of xenograft tumors. When ESAlowCD44-/low cells were allowed to aggregate and re-form floating-spheres, the biological/proliferative hierarchy of parental HCT116 spheres was reconstituted, in terms of ESA and CD44 expression. Thus, HCT116 cells have plasticity when they are set in floating-spheres, suggesting that maintenance of the HCT116 cell line conforms to a stochastic model, not a CSC model.

Original languageEnglish
Pages (from-to)2275-2282
Number of pages8
JournalCancer Science
Volume100
Issue number12
DOIs
Publication statusPublished - 2009 Dec

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Neoplastic Stem Cells
Colonic Neoplasms
HCT116 Cells
Maintenance
Cell Line
Neoplasms
Population
Differentiation Antigens
Heterografts
Agar
Phenotype
Incidence
Epithelial Cell Adhesion Molecule

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Maintenance of HCT116 colon cancer cell line conforms to a stochastic model but not a cancer stem cell model. / Kai, Kazuharu; Nagano, Osamu; Sugihara, Eiji; Arima, Yoshimi; Sampetrean, Oltea; Ishimoto, Takatsugu; Nakanishi, Masaya; Ueno, Naoto T.; Iwase, Hirotaka; Saya, Hideyuki.

In: Cancer Science, Vol. 100, No. 12, 12.2009, p. 2275-2282.

Research output: Contribution to journalArticle

Kai, Kazuharu ; Nagano, Osamu ; Sugihara, Eiji ; Arima, Yoshimi ; Sampetrean, Oltea ; Ishimoto, Takatsugu ; Nakanishi, Masaya ; Ueno, Naoto T. ; Iwase, Hirotaka ; Saya, Hideyuki. / Maintenance of HCT116 colon cancer cell line conforms to a stochastic model but not a cancer stem cell model. In: Cancer Science. 2009 ; Vol. 100, No. 12. pp. 2275-2282.
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