TY - JOUR
T1 - Major intrinsic polypeptide (MIP26K) from human lens membrane
T2 - Characterization of low-molecular-weight forms in the aging human lens
AU - Takemoto, L.
AU - Takehana, M.
N1 - Funding Information:
AGKNOV~LEDGMENTS This research was suppo~ by grants from the National Institutes of Health to L.T. We thank ~r J~ H0rwitz for his critical reading and comments on thismanuscript.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1986/10
Y1 - 1986/10
N2 - Antisera made against a C-terminal octapeptide of bovine MIP26K and against an N-terminal nonapeptide from the same protein have been used to determine the location of age-dependent cleavage sites of MIP26K in the human lens. Neither the C-terminal antiserum (anti-MIP26K256-263) nor the N-terminal antiserum (anti-MIP26K1-9) binds to the 22000 MW form of MIP26K, suggesting cleavage from both the N- and C-terminus during lens aging. Anti-MIP26K256-263, but not anti-MIP26K1-9, binds to 20000 and 15000 MW forms of MIP26K, demonstrating that age-dependent production of these forms occurs by cleavage from the N-terminal side of the molecule. Together, these results show that age-dependent processing of MIP26K in the human lens occurs from both ends of the molecule, with cleavage from the N-terminal end being mainly responsible for production of the lower-molecular-weight 20000 and 15000 MW components.
AB - Antisera made against a C-terminal octapeptide of bovine MIP26K and against an N-terminal nonapeptide from the same protein have been used to determine the location of age-dependent cleavage sites of MIP26K in the human lens. Neither the C-terminal antiserum (anti-MIP26K256-263) nor the N-terminal antiserum (anti-MIP26K1-9) binds to the 22000 MW form of MIP26K, suggesting cleavage from both the N- and C-terminus during lens aging. Anti-MIP26K256-263, but not anti-MIP26K1-9, binds to 20000 and 15000 MW forms of MIP26K, demonstrating that age-dependent production of these forms occurs by cleavage from the N-terminal side of the molecule. Together, these results show that age-dependent processing of MIP26K in the human lens occurs from both ends of the molecule, with cleavage from the N-terminal end being mainly responsible for production of the lower-molecular-weight 20000 and 15000 MW components.
KW - MIP26K
KW - lens membrane
KW - peptide antisera
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U2 - 10.1016/S0014-4835(86)80032-X
DO - 10.1016/S0014-4835(86)80032-X
M3 - Article
C2 - 3792465
AN - SCOPUS:0022978546
VL - 43
SP - 661
EP - 667
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
IS - 4
ER -