MALDI-based imaging mass spectrometry revealed abnormal distribution of phospholipids in colon cancer liver metastasis

Shuichi Shimma, Yuki Sugiura, Takahiro Hayasaka, Yutaka Hoshikawa, Tetsuo Noda, Mitsutoshi Setou

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

We present the results of matrix-assisted laser desorption/ionization (MALDI) imaging and direct molecular identification using tandem mass spectrometry (MS/MS) in colon cancer liver metastasis. Cancer tissue was removed from a Japanese patient and frozen immediately without any fixations. The sections were sliced to a thickness of 3 μm. The matrix for lipid ionization was 2,6-dihydroxy acetophenone. The matrix solution was applied with an airbrush into a thin uniform matrix layer on the tissue surface. After two-dimensional laser scanning, the images were reconstructed as a function of m/z from a few hundred obtained spectra. In the obtained images, the existence of molecules was represented by a pseudo-color corresponding to the signal intensity. In a feasibility study, we picked up a localized signal, m/z 725 in a cancerous area. The MS/MS result suggested that m/z 725 was sphingomyelin(16:0)+Na. Thus, we successfully show the feasibility of MALDI imaging as a tool for the analysis of pathological specimens.

Original languageEnglish
Pages (from-to)98-103
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume855
Issue number1 SPEC. ISS.
DOIs
Publication statusPublished - 2007 Aug 1
Externally publishedYes

Fingerprint

Liver Neoplasms
Liver
Colonic Neoplasms
Ionization
Mass spectrometry
Desorption
Mass Spectrometry
Phospholipids
Lasers
Neoplasm Metastasis
Imaging techniques
Tissue
Molecular Imaging
Sphingomyelins
Feasibility Studies
Tandem Mass Spectrometry
Color
Scanning
Lipids
Molecules

Keywords

  • Aging
  • Colon cancer
  • Imaging mass spectrometry
  • Molecular imaging
  • Phospholipids
  • Sphingomyelin

ASJC Scopus subject areas

  • Biochemistry

Cite this

MALDI-based imaging mass spectrometry revealed abnormal distribution of phospholipids in colon cancer liver metastasis. / Shimma, Shuichi; Sugiura, Yuki; Hayasaka, Takahiro; Hoshikawa, Yutaka; Noda, Tetsuo; Setou, Mitsutoshi.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 855, No. 1 SPEC. ISS., 01.08.2007, p. 98-103.

Research output: Contribution to journalArticle

Shimma, Shuichi ; Sugiura, Yuki ; Hayasaka, Takahiro ; Hoshikawa, Yutaka ; Noda, Tetsuo ; Setou, Mitsutoshi. / MALDI-based imaging mass spectrometry revealed abnormal distribution of phospholipids in colon cancer liver metastasis. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2007 ; Vol. 855, No. 1 SPEC. ISS. pp. 98-103.
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