TY - JOUR
T1 - MALDI-based imaging mass spectrometry revealed abnormal distribution of phospholipids in colon cancer liver metastasis
AU - Shimma, Shuichi
AU - Sugiura, Yuki
AU - Hayasaka, Takahiro
AU - Hoshikawa, Yutaka
AU - Noda, Tetsuo
AU - Setou, Mitsutoshi
N1 - Funding Information:
The authors would like to thank M. Sugiyama in the Genome Center of JFCR for her technical support with histochemical staining. This work was supported by a Grant-in-Aid for SENTAN (M.S.) from Japan Science and Technology Agency.
PY - 2007/8/1
Y1 - 2007/8/1
N2 - We present the results of matrix-assisted laser desorption/ionization (MALDI) imaging and direct molecular identification using tandem mass spectrometry (MS/MS) in colon cancer liver metastasis. Cancer tissue was removed from a Japanese patient and frozen immediately without any fixations. The sections were sliced to a thickness of 3 μm. The matrix for lipid ionization was 2,6-dihydroxy acetophenone. The matrix solution was applied with an airbrush into a thin uniform matrix layer on the tissue surface. After two-dimensional laser scanning, the images were reconstructed as a function of m/z from a few hundred obtained spectra. In the obtained images, the existence of molecules was represented by a pseudo-color corresponding to the signal intensity. In a feasibility study, we picked up a localized signal, m/z 725 in a cancerous area. The MS/MS result suggested that m/z 725 was sphingomyelin(16:0)+Na. Thus, we successfully show the feasibility of MALDI imaging as a tool for the analysis of pathological specimens.
AB - We present the results of matrix-assisted laser desorption/ionization (MALDI) imaging and direct molecular identification using tandem mass spectrometry (MS/MS) in colon cancer liver metastasis. Cancer tissue was removed from a Japanese patient and frozen immediately without any fixations. The sections were sliced to a thickness of 3 μm. The matrix for lipid ionization was 2,6-dihydroxy acetophenone. The matrix solution was applied with an airbrush into a thin uniform matrix layer on the tissue surface. After two-dimensional laser scanning, the images were reconstructed as a function of m/z from a few hundred obtained spectra. In the obtained images, the existence of molecules was represented by a pseudo-color corresponding to the signal intensity. In a feasibility study, we picked up a localized signal, m/z 725 in a cancerous area. The MS/MS result suggested that m/z 725 was sphingomyelin(16:0)+Na. Thus, we successfully show the feasibility of MALDI imaging as a tool for the analysis of pathological specimens.
KW - Aging
KW - Colon cancer
KW - Imaging mass spectrometry
KW - Molecular imaging
KW - Phospholipids
KW - Sphingomyelin
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U2 - 10.1016/j.jchromb.2007.02.037
DO - 10.1016/j.jchromb.2007.02.037
M3 - Article
C2 - 17369111
AN - SCOPUS:34447578029
VL - 855
SP - 98
EP - 103
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1 SPEC. ISS.
ER -
