Mapping of the three-dimensional lymphatic microvasculature in bladder tumours using light-sheet microscopy

Nobuyuki Tanaka, Dagmara Kaczynska, Shigeaki Kanatani, Cecilia Sahlgren, Przemysław Mitura, Andrzej Stepulak, Ayako Miyakawa, Peter Wiklund, Per Uhlén

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Cancers are heterogeneous and contain various types of irregular structures that can go undetected when examining them with standard two-dimensional microscopes. Studies of intricate networks of vasculature systems, e.g., the tumour lymphatic microvessels, benefit largely from three-dimensional imaging data analysis. Methods: The new DIPCO (Diagnosing Immunolabeled Paraffin-Embedded Cleared Organs) imaging platform uses three-dimensional light-sheet microscopy and whole-mount immunolabelling of cleared samples to study proteins and micro-anatomies deep inside of tumours. Results: Here, we uncovered the whole three-dimensional lymphatic microvasculature of formalin-fixed paraffin-embedded (FFPE) tumours from a cohort of 30 patients with bladder cancer. Our results revealed more heterogeneous spatial deviations in more advanced bladder tumours. We also showed that three-dimensional imaging could determine tumour stage and identify vascular or lymphatic system invasion with higher accuracy than standard two-dimensional histological diagnostic methods. There was no association between sample storage times and outcomes, demonstrating that the DIPCO pipeline could be successfully applied on old FFPE samples. Conclusions: Studying tumour samples with three-dimensional imaging could help us understand the pathological nature of cancers and provide essential information that might improve the accuracy of cancer staging.

Original languageEnglish
Pages (from-to)995-999
Number of pages5
JournalBritish Journal of Cancer
Volume118
Issue number7
DOIs
Publication statusPublished - 2018 Apr 1

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Microvessels
Urinary Bladder Neoplasms
Microscopy
Light
Paraffin
Three-Dimensional Imaging
Neoplasms
Formaldehyde
Lymphatic System
Neoplasm Staging
Blood Vessels
Anatomy
Proteins

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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Mapping of the three-dimensional lymphatic microvasculature in bladder tumours using light-sheet microscopy. / Tanaka, Nobuyuki; Kaczynska, Dagmara; Kanatani, Shigeaki; Sahlgren, Cecilia; Mitura, Przemysław; Stepulak, Andrzej; Miyakawa, Ayako; Wiklund, Peter; Uhlén, Per.

In: British Journal of Cancer, Vol. 118, No. 7, 01.04.2018, p. 995-999.

Research output: Contribution to journalArticle

Tanaka, N, Kaczynska, D, Kanatani, S, Sahlgren, C, Mitura, P, Stepulak, A, Miyakawa, A, Wiklund, P & Uhlén, P 2018, 'Mapping of the three-dimensional lymphatic microvasculature in bladder tumours using light-sheet microscopy', British Journal of Cancer, vol. 118, no. 7, pp. 995-999. https://doi.org/10.1038/s41416-018-0016-y
Tanaka, Nobuyuki ; Kaczynska, Dagmara ; Kanatani, Shigeaki ; Sahlgren, Cecilia ; Mitura, Przemysław ; Stepulak, Andrzej ; Miyakawa, Ayako ; Wiklund, Peter ; Uhlén, Per. / Mapping of the three-dimensional lymphatic microvasculature in bladder tumours using light-sheet microscopy. In: British Journal of Cancer. 2018 ; Vol. 118, No. 7. pp. 995-999.
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