Marked differences in differentiation propensity among human embryonic stem cell lines

Kenji Osafune, Leslie Caron, Malgorzata Borowiak, Rita J. Martinez, Claire S. Fitz-Gerald, Yasunori Sato, Chad A. Cowan, Kenneth R. Chien, Douglas A. Melton

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Abstract

The differentiation potential of 17 human embryonic stem (hES) cell lines was compared. Some lines exhibit a marked propensity to differentiate into specific lineages, often with >100-fold differences in lineage-specific gene expression. For example, HUES 8 is best for pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial differences in developmental potential among hES cell lines point to the importance of screening and deriving lines for lineage-specific differentiation.

Original languageEnglish
Pages (from-to)313-315
Number of pages3
JournalNature Biotechnology
Volume26
Issue number3
DOIs
Publication statusPublished - 2008 Mar 1

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ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Osafune, K., Caron, L., Borowiak, M., Martinez, R. J., Fitz-Gerald, C. S., Sato, Y., Cowan, C. A., Chien, K. R., & Melton, D. A. (2008). Marked differences in differentiation propensity among human embryonic stem cell lines. Nature Biotechnology, 26(3), 313-315. https://doi.org/10.1038/nbt1383