Marked differences in differentiation propensity among human embryonic stem cell lines

Kenji Osafune, Leslie Caron, Malgorzata Borowiak, Rita J. Martinez, Claire S. Fitz-Gerald, Yasunori Sato, Chad A. Cowan, Kenneth R. Chien, Douglas A. Melton

Research output: Contribution to journalArticle

561 Citations (Scopus)

Abstract

The differentiation potential of 17 human embryonic stem (hES) cell lines was compared. Some lines exhibit a marked propensity to differentiate into specific lineages, often with >100-fold differences in lineage-specific gene expression. For example, HUES 8 is best for pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial differences in developmental potential among hES cell lines point to the importance of screening and deriving lines for lineage-specific differentiation.

Original languageEnglish
Pages (from-to)313-315
Number of pages3
JournalNature Biotechnology
Volume26
Issue number3
DOIs
Publication statusPublished - 2008 Mar 1
Externally publishedYes

Fingerprint

Stem cells
Cell Line
Cardiac Myocytes
Gene expression
Screening
Gene Expression
Human Embryonic Stem Cells

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Osafune, K., Caron, L., Borowiak, M., Martinez, R. J., Fitz-Gerald, C. S., Sato, Y., ... Melton, D. A. (2008). Marked differences in differentiation propensity among human embryonic stem cell lines. Nature Biotechnology, 26(3), 313-315. https://doi.org/10.1038/nbt1383

Marked differences in differentiation propensity among human embryonic stem cell lines. / Osafune, Kenji; Caron, Leslie; Borowiak, Malgorzata; Martinez, Rita J.; Fitz-Gerald, Claire S.; Sato, Yasunori; Cowan, Chad A.; Chien, Kenneth R.; Melton, Douglas A.

In: Nature Biotechnology, Vol. 26, No. 3, 01.03.2008, p. 313-315.

Research output: Contribution to journalArticle

Osafune, K, Caron, L, Borowiak, M, Martinez, RJ, Fitz-Gerald, CS, Sato, Y, Cowan, CA, Chien, KR & Melton, DA 2008, 'Marked differences in differentiation propensity among human embryonic stem cell lines', Nature Biotechnology, vol. 26, no. 3, pp. 313-315. https://doi.org/10.1038/nbt1383
Osafune K, Caron L, Borowiak M, Martinez RJ, Fitz-Gerald CS, Sato Y et al. Marked differences in differentiation propensity among human embryonic stem cell lines. Nature Biotechnology. 2008 Mar 1;26(3):313-315. https://doi.org/10.1038/nbt1383
Osafune, Kenji ; Caron, Leslie ; Borowiak, Malgorzata ; Martinez, Rita J. ; Fitz-Gerald, Claire S. ; Sato, Yasunori ; Cowan, Chad A. ; Chien, Kenneth R. ; Melton, Douglas A. / Marked differences in differentiation propensity among human embryonic stem cell lines. In: Nature Biotechnology. 2008 ; Vol. 26, No. 3. pp. 313-315.
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