Marked differences in differentiation propensity among human embryonic stem cell lines

Kenji Osafune; Leslie Caron; Malgorzata Borowiak; Rita J. Martinez; Claire S. Fitz-Gerald; Yasunori Sato; Chad A. Cowan; Kenneth R. Chien; Douglas A. Melton

doi:10.1038/nbt1383

Marked differences in differentiation propensity among human embryonic stem cell lines

Kenji Osafune, Leslie Caron, Malgorzata Borowiak, Rita J. Martinez, Claire S. Fitz-Gerald, Yasunori Sato, Chad A. Cowan, Kenneth R. Chien, Douglas A. Melton

Research output: Contribution to journal › Article › peer-review

323 Citations (Scopus)

Abstract

The differentiation potential of 17 human embryonic stem (hES) cell lines was compared. Some lines exhibit a marked propensity to differentiate into specific lineages, often with >100-fold differences in lineage-specific gene expression. For example, HUES 8 is best for pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial differences in developmental potential among hES cell lines point to the importance of screening and deriving lines for lineage-specific differentiation.

Original language	English
Pages (from-to)	313-315
Number of pages	3
Journal	Nature Biotechnology
Volume	26
Issue number	3
DOIs	https://doi.org/10.1038/nbt1383
Publication status	Published - 2008 Mar
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

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10.1038/nbt1383

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abstract = "The differentiation potential of 17 human embryonic stem (hES) cell lines was compared. Some lines exhibit a marked propensity to differentiate into specific lineages, often with >100-fold differences in lineage-specific gene expression. For example, HUES 8 is best for pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial differences in developmental potential among hES cell lines point to the importance of screening and deriving lines for lineage-specific differentiation.",

author = "Kenji Osafune and Leslie Caron and Malgorzata Borowiak and Martinez, {Rita J.} and Fitz-Gerald, {Claire S.} and Yasunori Sato and Cowan, {Chad A.} and Chien, {Kenneth R.} and Melton, {Douglas A.}",

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AU - Caron, Leslie

AU - Borowiak, Malgorzata

AU - Martinez, Rita J.

AU - Fitz-Gerald, Claire S.

AU - Sato, Yasunori

AU - Cowan, Chad A.

AU - Chien, Kenneth R.

AU - Melton, Douglas A.

PY - 2008/3

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N2 - The differentiation potential of 17 human embryonic stem (hES) cell lines was compared. Some lines exhibit a marked propensity to differentiate into specific lineages, often with >100-fold differences in lineage-specific gene expression. For example, HUES 8 is best for pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial differences in developmental potential among hES cell lines point to the importance of screening and deriving lines for lineage-specific differentiation.

AB - The differentiation potential of 17 human embryonic stem (hES) cell lines was compared. Some lines exhibit a marked propensity to differentiate into specific lineages, often with >100-fold differences in lineage-specific gene expression. For example, HUES 8 is best for pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial differences in developmental potential among hES cell lines point to the importance of screening and deriving lines for lineage-specific differentiation.

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Marked differences in differentiation propensity among human embryonic stem cell lines

Abstract

ASJC Scopus subject areas

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