Markedly reduced axonal potassium channel expression in human sporadic amyotrophic lateral sclerosis: An immunohistochemical study

Kazumoto Shibuya, Sonoko Misawa, Kimihito Arai, Miho Nakata, Kazuaki Kanai, Yasumasa Yoshiyama, Kimiko Ito, Sagiri Isose, Yu ichi Noto, Saiko Nasu, Yukari Sekiguchi, Yumi Fujimaki, Shigeki Ohmori, Hiroshi Kitamura, Yasunori Sato, Satoshi Kuwabara

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Fasciculations are characteristic features of amyotrophic lateral sclerosis (ALS), suggesting abnormally increased excitability of motor axons. Previous nerve excitability studies have shown reduced axonal potassium currents in ALS patients that may contribute to the hyperexcitability and thereby generation of fasciculations. To clarify changes in axonal ion channel expression in motor axons of ALS, we performed immunohistochemistry of potassium and sodium channels in the C7 and L5 ventral/dorsal roots obtained from five autopsy cases of sporadic ALS. Compared to controls, the immunoreactivity of potassium channels (Kv1.2) was markedly reduced in the ventral roots, but normal in the dorsal roots of all the ALS patients. Nodal sodium channel expression was not significantly different in ALS patients and control subjects. Our results show prominently reduced expression of axonal potassium channels, and provide the neuropathological and biological basis for decreased accommodative potassium currents in motor axons of ALS patients. The axonal hyperexcitability would lead to generation of fasciculations, and possibly enhances motor neuron death in ALS.

Original languageEnglish
Pages (from-to)149-153
Number of pages5
JournalExperimental Neurology
Volume232
Issue number2
DOIs
Publication statusPublished - 2011 Dec 1
Externally publishedYes

Fingerprint

Potassium Channels
Amyotrophic Lateral Sclerosis
Spinal Nerve Roots
Fasciculation
Axons
Sodium Channels
Kv1.2 Potassium Channel
Potassium
Motor Neurons
Amyotrophic lateral sclerosis 1
Ion Channels
Autopsy
Immunohistochemistry

Keywords

  • Amyotrophic lateral sclerosis
  • Axonal hyperexcitability
  • Fasciculation
  • Motor axon
  • Potassium channel

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Markedly reduced axonal potassium channel expression in human sporadic amyotrophic lateral sclerosis : An immunohistochemical study. / Shibuya, Kazumoto; Misawa, Sonoko; Arai, Kimihito; Nakata, Miho; Kanai, Kazuaki; Yoshiyama, Yasumasa; Ito, Kimiko; Isose, Sagiri; Noto, Yu ichi; Nasu, Saiko; Sekiguchi, Yukari; Fujimaki, Yumi; Ohmori, Shigeki; Kitamura, Hiroshi; Sato, Yasunori; Kuwabara, Satoshi.

In: Experimental Neurology, Vol. 232, No. 2, 01.12.2011, p. 149-153.

Research output: Contribution to journalArticle

Shibuya, K, Misawa, S, Arai, K, Nakata, M, Kanai, K, Yoshiyama, Y, Ito, K, Isose, S, Noto, YI, Nasu, S, Sekiguchi, Y, Fujimaki, Y, Ohmori, S, Kitamura, H, Sato, Y & Kuwabara, S 2011, 'Markedly reduced axonal potassium channel expression in human sporadic amyotrophic lateral sclerosis: An immunohistochemical study', Experimental Neurology, vol. 232, no. 2, pp. 149-153. https://doi.org/10.1016/j.expneurol.2011.08.015
Shibuya, Kazumoto ; Misawa, Sonoko ; Arai, Kimihito ; Nakata, Miho ; Kanai, Kazuaki ; Yoshiyama, Yasumasa ; Ito, Kimiko ; Isose, Sagiri ; Noto, Yu ichi ; Nasu, Saiko ; Sekiguchi, Yukari ; Fujimaki, Yumi ; Ohmori, Shigeki ; Kitamura, Hiroshi ; Sato, Yasunori ; Kuwabara, Satoshi. / Markedly reduced axonal potassium channel expression in human sporadic amyotrophic lateral sclerosis : An immunohistochemical study. In: Experimental Neurology. 2011 ; Vol. 232, No. 2. pp. 149-153.
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