Maternal separation stress drastically decreases expression of transthyretin in the brains of adult rat offspring

Kazuhisa Kohda, Seiichiro Jinde, Kazuya Iwamoto, Miki Bundo, Nobumasa Kato, Tadafumi Kato

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Adversity in early life has been recognized as a risk factor for psychiatric disorders. In experimental animals, maternal separation (MS) during the neonatal period has been shown to be critical for susceptibility to stress in adult offspring. In this study, we used DNA microarray analysis of rat hippocampal samples to investigate differential gene expression caused by 8-hour MS (MS-8h) every other day during the neonatal period. We found 15 up-regulated and 9 down-regulated genes. We added samples from a daily 15-minute MS (MS-15m) group and performed quantitative real-time PCR to validate the results. Expression of transthyretin (TTR), which is specifically expressed in the choroid plexus (CP), was drastically reduced in the MS-8h group. Two other CP-enriched genes, angiotensin I converting enzyme I and insulin-like growth factor II (IGF-II), were also significantly down-regulated in the MS-8h rats, while significant reduction of IGF-II expression was also found in the MS-15m group. These MS-induced differential gene expressions could be involved in the molecular mechanisms of stress susceptibility. Our findings indicate that the CP, in addition to the neuronal and glial system, might play an important role in determining stress susceptibility.

Original languageEnglish
Pages (from-to)201-208
Number of pages8
JournalInternational Journal of Neuropsychopharmacology
Volume9
Issue number2
DOIs
Publication statusPublished - 2006 Apr

Fingerprint

Prealbumin
Mothers
Brain
Choroid Plexus
Insulin-Like Growth Factor II
Gene Expression
Peptidyl-Dipeptidase A
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Neuroglia
Genes
Psychiatry
Real-Time Polymerase Chain Reaction

Keywords

  • Choroid plexus
  • DNA microarray
  • Hippocampus
  • Maternal separation
  • Transthyretin

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Neuropsychology and Physiological Psychology
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Maternal separation stress drastically decreases expression of transthyretin in the brains of adult rat offspring. / Kohda, Kazuhisa; Jinde, Seiichiro; Iwamoto, Kazuya; Bundo, Miki; Kato, Nobumasa; Kato, Tadafumi.

In: International Journal of Neuropsychopharmacology, Vol. 9, No. 2, 04.2006, p. 201-208.

Research output: Contribution to journalArticle

Kohda, Kazuhisa ; Jinde, Seiichiro ; Iwamoto, Kazuya ; Bundo, Miki ; Kato, Nobumasa ; Kato, Tadafumi. / Maternal separation stress drastically decreases expression of transthyretin in the brains of adult rat offspring. In: International Journal of Neuropsychopharmacology. 2006 ; Vol. 9, No. 2. pp. 201-208.
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