Matrix-assisted laser desorption/ionization and nanoparticle-based imaging mass spectrometry for small metabolites: A practical protocol

Yuki Sugiura, Mitsutoshi Setou

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS, also referred to as mass spectrometry imaging [MSI]) enables visualization of the distribution of biomolecules with varied and vast structures in tissue sections. This emerging imaging technique was initially developed as a tool for protein imaging; however, the number of studies reporting imaging of small organic molecules has recently increased. IMS is an effective technique for the visualization of endogenous small metabolites, especially lipids, facilitated by the unique advantages of mass spectrometry-based molecular detection. Despite the promising capability of MALDI-IMS for imaging small metabolites, this technique still has several issues, especially in spatial resolution. One of the critical limitations of the spatial resolution of MALDI-IMS is the size of the organic matrix crystal and the analyte migration during the matrix application process. To overcome these problems, we reported a nanoparticle (NP)-assisted laser desorption/ionization (nano-PALDI)-based IMS, in which the matrix crystallization process is eliminated. In this chapter, a practical protocol for MALDI-IMS of lipids is outlined. In addition, as an attractive alternative to MALDI-based IMS, we also present nanoparticle-based IMS that improves spatial resolution.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages173-195
Number of pages23
Volume656
DOIs
Publication statusPublished - 2010
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume656
ISSN (Print)10643745

Fingerprint

Nanoparticles
Mass Spectrometry
Lasers
Lipids
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Crystallization
Proteins

Keywords

  • Imaging mass spectrometry
  • lipids
  • MALDI
  • matrix-assisted laser desorption/ionization
  • nanoparticles
  • small molecules

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Matrix-assisted laser desorption/ionization and nanoparticle-based imaging mass spectrometry for small metabolites : A practical protocol. / Sugiura, Yuki; Setou, Mitsutoshi.

Methods in Molecular Biology. Vol. 656 2010. p. 173-195 (Methods in Molecular Biology; Vol. 656).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sugiura, Yuki ; Setou, Mitsutoshi. / Matrix-assisted laser desorption/ionization and nanoparticle-based imaging mass spectrometry for small metabolites : A practical protocol. Methods in Molecular Biology. Vol. 656 2010. pp. 173-195 (Methods in Molecular Biology).
@inbook{4676a106482544e5b926e7701c42ebe7,
title = "Matrix-assisted laser desorption/ionization and nanoparticle-based imaging mass spectrometry for small metabolites: A practical protocol",
abstract = "Matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS, also referred to as mass spectrometry imaging [MSI]) enables visualization of the distribution of biomolecules with varied and vast structures in tissue sections. This emerging imaging technique was initially developed as a tool for protein imaging; however, the number of studies reporting imaging of small organic molecules has recently increased. IMS is an effective technique for the visualization of endogenous small metabolites, especially lipids, facilitated by the unique advantages of mass spectrometry-based molecular detection. Despite the promising capability of MALDI-IMS for imaging small metabolites, this technique still has several issues, especially in spatial resolution. One of the critical limitations of the spatial resolution of MALDI-IMS is the size of the organic matrix crystal and the analyte migration during the matrix application process. To overcome these problems, we reported a nanoparticle (NP)-assisted laser desorption/ionization (nano-PALDI)-based IMS, in which the matrix crystallization process is eliminated. In this chapter, a practical protocol for MALDI-IMS of lipids is outlined. In addition, as an attractive alternative to MALDI-based IMS, we also present nanoparticle-based IMS that improves spatial resolution.",
keywords = "Imaging mass spectrometry, lipids, MALDI, matrix-assisted laser desorption/ionization, nanoparticles, small molecules",
author = "Yuki Sugiura and Mitsutoshi Setou",
year = "2010",
doi = "10.1007/978-1-60761-746-4_10",
language = "English",
isbn = "9781607617457",
volume = "656",
series = "Methods in Molecular Biology",
pages = "173--195",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Matrix-assisted laser desorption/ionization and nanoparticle-based imaging mass spectrometry for small metabolites

T2 - A practical protocol

AU - Sugiura, Yuki

AU - Setou, Mitsutoshi

PY - 2010

Y1 - 2010

N2 - Matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS, also referred to as mass spectrometry imaging [MSI]) enables visualization of the distribution of biomolecules with varied and vast structures in tissue sections. This emerging imaging technique was initially developed as a tool for protein imaging; however, the number of studies reporting imaging of small organic molecules has recently increased. IMS is an effective technique for the visualization of endogenous small metabolites, especially lipids, facilitated by the unique advantages of mass spectrometry-based molecular detection. Despite the promising capability of MALDI-IMS for imaging small metabolites, this technique still has several issues, especially in spatial resolution. One of the critical limitations of the spatial resolution of MALDI-IMS is the size of the organic matrix crystal and the analyte migration during the matrix application process. To overcome these problems, we reported a nanoparticle (NP)-assisted laser desorption/ionization (nano-PALDI)-based IMS, in which the matrix crystallization process is eliminated. In this chapter, a practical protocol for MALDI-IMS of lipids is outlined. In addition, as an attractive alternative to MALDI-based IMS, we also present nanoparticle-based IMS that improves spatial resolution.

AB - Matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS, also referred to as mass spectrometry imaging [MSI]) enables visualization of the distribution of biomolecules with varied and vast structures in tissue sections. This emerging imaging technique was initially developed as a tool for protein imaging; however, the number of studies reporting imaging of small organic molecules has recently increased. IMS is an effective technique for the visualization of endogenous small metabolites, especially lipids, facilitated by the unique advantages of mass spectrometry-based molecular detection. Despite the promising capability of MALDI-IMS for imaging small metabolites, this technique still has several issues, especially in spatial resolution. One of the critical limitations of the spatial resolution of MALDI-IMS is the size of the organic matrix crystal and the analyte migration during the matrix application process. To overcome these problems, we reported a nanoparticle (NP)-assisted laser desorption/ionization (nano-PALDI)-based IMS, in which the matrix crystallization process is eliminated. In this chapter, a practical protocol for MALDI-IMS of lipids is outlined. In addition, as an attractive alternative to MALDI-based IMS, we also present nanoparticle-based IMS that improves spatial resolution.

KW - Imaging mass spectrometry

KW - lipids

KW - MALDI

KW - matrix-assisted laser desorption/ionization

KW - nanoparticles

KW - small molecules

UR - http://www.scopus.com/inward/record.url?scp=78149470608&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78149470608&partnerID=8YFLogxK

U2 - 10.1007/978-1-60761-746-4_10

DO - 10.1007/978-1-60761-746-4_10

M3 - Chapter

C2 - 20680591

AN - SCOPUS:78149470608

SN - 9781607617457

VL - 656

T3 - Methods in Molecular Biology

SP - 173

EP - 195

BT - Methods in Molecular Biology

ER -