Matrix-assisted laser desorption/ionization and nanoparticle-based imaging mass spectrometry for small metabolites: A practical protocol

Yuki Sugiura, Mitsutoshi Setou

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS, also referred to as mass spectrometry imaging [MSI]) enables visualization of the distribution of biomolecules with varied and vast structures in tissue sections. This emerging imaging technique was initially developed as a tool for protein imaging; however, the number of studies reporting imaging of small organic molecules has recently increased. IMS is an effective technique for the visualization of endogenous small metabolites, especially lipids, facilitated by the unique advantages of mass spectrometry-based molecular detection. Despite the promising capability of MALDI-IMS for imaging small metabolites, this technique still has several issues, especially in spatial resolution. One of the critical limitations of the spatial resolution of MALDI-IMS is the size of the organic matrix crystal and the analyte migration during the matrix application process. To overcome these problems, we reported a nanoparticle (NP)-assisted laser desorption/ionization (nano-PALDI)-based IMS, in which the matrix crystallization process is eliminated. In this chapter, a practical protocol for MALDI-IMS of lipids is outlined. In addition, as an attractive alternative to MALDI-based IMS, we also present nanoparticle-based IMS that improves spatial resolution.

Original languageEnglish
Title of host publicationMass Spectrometry Imaging
Subtitle of host publicationPrinciples and Protocols
EditorsStanislav Rubakhin, Jonathan Sweedler
Pages173-195
Number of pages23
DOIs
Publication statusPublished - 2010
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume656
ISSN (Print)1064-3745

Keywords

  • Imaging mass spectrometry
  • MALDI
  • lipids
  • matrix-assisted laser desorption/ionization
  • nanoparticles
  • small molecules

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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