Matrix metalloproteinase 7 (matrilysin) from human rectal carcinoma cells. Activation of the precursor, interaction with other matrix metalloproteinases and enzymic properties

K. Imai, Y. Yokohama, I. Nakanishi, E. Ohuchi, Y. Fujii, N. Nakai, Y. Okada

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Matrix metalloproteinase 7 (MMP-7) has been purified as an inactive zymogen of M(r) 28,000 (proMMP-7) from the culture medium of CAR-1 human rectal carcinoma cells. The NH2-terminal sequence of proMMP-7 is Lys-Pro- Lys-Pro-Gln-Glu, which is identical to that of matrilysin. The zymogen is activated by 4-aminophenylmercuric acetate (APMA), yielding an intermediate form of M(r) 21,000 and an active species of M(r) 19,000 which shows the new NH2-terminal sequence of Tyr78-Ser-Leu-Phe-Pro-Asn-Ser. Although trypsin fully activates the zymogen, the activation rate by plasmin or leukocyte elastase is confined to Å50%. ProMMP-7 can be activated by MMP-3 (stromelysin 1) to its full activity in a single-step mechanism and generates the same NH2 terminus obtained by APMA activation, whereas MMP-1 (tissue collagenase), MMP-2 (gelatinase A), and MMP-9 (gelatinase B) do not have such an effect. On the other hand, proMMP-1 is activated by MMP-7 to an activity similar to that obtained by APMA and the activation by MMP-7 is enhanced up to Å6.5-fold in the presence of APMA. This enhanced activity is donated by specific cleavage at the Glns80-Phe81 bond of proMMP-1. MMP-7 can also activate proMMP-9 up to Å50% of the full activity with a new NH2 terminus of Leu16-Arg-Thr-(Asn)-Leu. Incubation of proMMP-2 or proMMP-3 with MMP-7 results in no activation of these proMMPs. MMP-7 degrades type IV collagen, laminin-1, fibronectin, proteoglycan, type I gelatin, and insoluble elastin. These results suggest that in vivo MMP-7 may play a role in degradation of extracellular matrix macromolecules in concert with MMP-1, -3, and -9 under pathological conditions.

Original languageEnglish
Pages (from-to)6691-6697
Number of pages7
JournalJournal of Biological Chemistry
Issue number12
Publication statusPublished - 1995 Jan 1


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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