Matrix metalloproteinase-9 and tensile strength of fetal membranes in uncomplicated labor

Kiyoshi Uchide, Hirohisa Ueno, Masaki Inoue, Akeml Sakai, Noboru Fujimoto, Yasunori Okada

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Objective: To analyze the relation between tensile strength and levels of matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, and tissue inhibitor of metalloproteinase-2 at a number of sites in human fetal membranes. Methods: Tensile strengths of fetal membranes from five women who delivered vaginally at term were measured by the method of modified force application. A piece of membrane at each measured site was then dissected, and the levels of matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, and tissue inhibitor of metalloproteinase-2 were measured by sandwich enzyme immunoassay. The relationship between tensile strength and enzyme levels was evaluated by Scheffé F test at a total of 81 sites on the five membranes. Results: The mean tensile strength of the membranes was 45.3 ±19.8 (mean ± standard deviation) mmHg/0.3 mm2 (n = 81). When the measured sites were divided according to tensile strength into four groups (<25, 25-49, 50-74, and ≥75 mmHg/0.3 mm2), the level of matrix metalloproteinase-9 (0.72 ± 0.82 nmol/g protein, n = 12) in the less than 25 mmHg/0.3 mm2 group was significantly higher than the other groups (0.35 ± 0.22, 0.28 ± 0.15, and 0.15 ± 0.08 nmol/g protein; n = 39, 23, and 7, respectively). The significance level was still higher when the molar ratio of matrix metalloproteinase-9 to tissue inhibitor of metalloproteinase-1 was used for comparison. Conclusion: An increased molar ratio of matrix metalloproteinase-9 to tissue inhibitor of metalloproteinase-1 might be related to decreased tensile strength of human fetal membranes in uncomplicated labor.

Original languageEnglish
Pages (from-to)851-855
Number of pages5
JournalObstetrics and Gynecology
Volume95
Issue number6 I
Publication statusPublished - 2000

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Extraembryonic Membranes
Tensile Strength
Matrix Metalloproteinase 9
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2
Membranes
Immunoenzyme Techniques
Proteins
Enzymes

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Uchide, K., Ueno, H., Inoue, M., Sakai, A., Fujimoto, N., & Okada, Y. (2000). Matrix metalloproteinase-9 and tensile strength of fetal membranes in uncomplicated labor. Obstetrics and Gynecology, 95(6 I), 851-855.

Matrix metalloproteinase-9 and tensile strength of fetal membranes in uncomplicated labor. / Uchide, Kiyoshi; Ueno, Hirohisa; Inoue, Masaki; Sakai, Akeml; Fujimoto, Noboru; Okada, Yasunori.

In: Obstetrics and Gynecology, Vol. 95, No. 6 I, 2000, p. 851-855.

Research output: Contribution to journalArticle

Uchide, K, Ueno, H, Inoue, M, Sakai, A, Fujimoto, N & Okada, Y 2000, 'Matrix metalloproteinase-9 and tensile strength of fetal membranes in uncomplicated labor', Obstetrics and Gynecology, vol. 95, no. 6 I, pp. 851-855.
Uchide K, Ueno H, Inoue M, Sakai A, Fujimoto N, Okada Y. Matrix metalloproteinase-9 and tensile strength of fetal membranes in uncomplicated labor. Obstetrics and Gynecology. 2000;95(6 I):851-855.
Uchide, Kiyoshi ; Ueno, Hirohisa ; Inoue, Masaki ; Sakai, Akeml ; Fujimoto, Noboru ; Okada, Yasunori. / Matrix metalloproteinase-9 and tensile strength of fetal membranes in uncomplicated labor. In: Obstetrics and Gynecology. 2000 ; Vol. 95, No. 6 I. pp. 851-855.
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AU - Uchide, Kiyoshi

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AU - Inoue, Masaki

AU - Sakai, Akeml

AU - Fujimoto, Noboru

AU - Okada, Yasunori

PY - 2000

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N2 - Objective: To analyze the relation between tensile strength and levels of matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, and tissue inhibitor of metalloproteinase-2 at a number of sites in human fetal membranes. Methods: Tensile strengths of fetal membranes from five women who delivered vaginally at term were measured by the method of modified force application. A piece of membrane at each measured site was then dissected, and the levels of matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, and tissue inhibitor of metalloproteinase-2 were measured by sandwich enzyme immunoassay. The relationship between tensile strength and enzyme levels was evaluated by Scheffé F test at a total of 81 sites on the five membranes. Results: The mean tensile strength of the membranes was 45.3 ±19.8 (mean ± standard deviation) mmHg/0.3 mm2 (n = 81). When the measured sites were divided according to tensile strength into four groups (<25, 25-49, 50-74, and ≥75 mmHg/0.3 mm2), the level of matrix metalloproteinase-9 (0.72 ± 0.82 nmol/g protein, n = 12) in the less than 25 mmHg/0.3 mm2 group was significantly higher than the other groups (0.35 ± 0.22, 0.28 ± 0.15, and 0.15 ± 0.08 nmol/g protein; n = 39, 23, and 7, respectively). The significance level was still higher when the molar ratio of matrix metalloproteinase-9 to tissue inhibitor of metalloproteinase-1 was used for comparison. Conclusion: An increased molar ratio of matrix metalloproteinase-9 to tissue inhibitor of metalloproteinase-1 might be related to decreased tensile strength of human fetal membranes in uncomplicated labor.

AB - Objective: To analyze the relation between tensile strength and levels of matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, and tissue inhibitor of metalloproteinase-2 at a number of sites in human fetal membranes. Methods: Tensile strengths of fetal membranes from five women who delivered vaginally at term were measured by the method of modified force application. A piece of membrane at each measured site was then dissected, and the levels of matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, and tissue inhibitor of metalloproteinase-2 were measured by sandwich enzyme immunoassay. The relationship between tensile strength and enzyme levels was evaluated by Scheffé F test at a total of 81 sites on the five membranes. Results: The mean tensile strength of the membranes was 45.3 ±19.8 (mean ± standard deviation) mmHg/0.3 mm2 (n = 81). When the measured sites were divided according to tensile strength into four groups (<25, 25-49, 50-74, and ≥75 mmHg/0.3 mm2), the level of matrix metalloproteinase-9 (0.72 ± 0.82 nmol/g protein, n = 12) in the less than 25 mmHg/0.3 mm2 group was significantly higher than the other groups (0.35 ± 0.22, 0.28 ± 0.15, and 0.15 ± 0.08 nmol/g protein; n = 39, 23, and 7, respectively). The significance level was still higher when the molar ratio of matrix metalloproteinase-9 to tissue inhibitor of metalloproteinase-1 was used for comparison. Conclusion: An increased molar ratio of matrix metalloproteinase-9 to tissue inhibitor of metalloproteinase-1 might be related to decreased tensile strength of human fetal membranes in uncomplicated labor.

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