Matrix metalloproteinase-9 and tensile strength of fetal membranes in uncomplicated labor

Kiyoshi Uchide, Hirohisa Ueno, Masaki Inoue, Akeml Sakai, Noboru Fujimoto, Yasunori Okada

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Abstract

Objective: To analyze the relation between tensile strength and levels of matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, and tissue inhibitor of metalloproteinase-2 at a number of sites in human fetal membranes. Methods: Tensile strengths of fetal membranes from five women who delivered vaginally at term were measured by the method of modified force application. A piece of membrane at each measured site was then dissected, and the levels of matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, and tissue inhibitor of metalloproteinase-2 were measured by sandwich enzyme immunoassay. The relationship between tensile strength and enzyme levels was evaluated by Scheffé F test at a total of 81 sites on the five membranes. Results: The mean tensile strength of the membranes was 45.3 ±19.8 (mean ± standard deviation) mmHg/0.3 mm2 (n = 81). When the measured sites were divided according to tensile strength into four groups (<25, 25-49, 50-74, and ≥75 mmHg/0.3 mm2), the level of matrix metalloproteinase-9 (0.72 ± 0.82 nmol/g protein, n = 12) in the less than 25 mmHg/0.3 mm2 group was significantly higher than the other groups (0.35 ± 0.22, 0.28 ± 0.15, and 0.15 ± 0.08 nmol/g protein; n = 39, 23, and 7, respectively). The significance level was still higher when the molar ratio of matrix metalloproteinase-9 to tissue inhibitor of metalloproteinase-1 was used for comparison. Conclusion: An increased molar ratio of matrix metalloproteinase-9 to tissue inhibitor of metalloproteinase-1 might be related to decreased tensile strength of human fetal membranes in uncomplicated labor.

Original languageEnglish
Pages (from-to)851-855
Number of pages5
JournalObstetrics and Gynecology
Volume95
Issue number6 I
Publication statusPublished - 2000

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ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Uchide, K., Ueno, H., Inoue, M., Sakai, A., Fujimoto, N., & Okada, Y. (2000). Matrix metalloproteinase-9 and tensile strength of fetal membranes in uncomplicated labor. Obstetrics and Gynecology, 95(6 I), 851-855.