Matrix metalloproteinases in blood from patients with LAM

Nao Odajima; Tomoko Betsuyaku; Yasuyuki Nasuhara; Hiromasa Inoue; Kuniaki Seyama; Masaharu Nishimura

doi:10.1016/j.rmed.2008.07.017

Matrix metalloproteinases in blood from patients with LAM

Nao Odajima, Tomoko Betsuyaku, Yasuyuki Nasuhara, Hiromasa Inoue, Kuniaki Seyama, Masaharu Nishimura

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Pulmonary lymphangioleiomyomatosis (LAM) is characterized by the proliferation of abnormal smooth muscle cells (LAM cells) and destruction of alveolar structure. Immunohistochemical studies suggest that excess matrix metalloproteinases (MMPs) synthesized by LAM cells function in the proteolytic mechanisms of this disease. We postulated MMP levels in the blood are elevated in LAM patients. Serum samples were collected from 36 LAM patients and 25 controls, and gelatinolytic activities were semi-quantified by gelatin zymography. The reliability of serum data for MMP-9 was confirmed by the measurement of MMP-9 concentration in plasma by enzyme-linked immunosorbent assay as well as by gelatin zymography. Serum levels of MMP-9 (0.7 ± 0.1 AU), but not MMP-2, were significantly elevated in LAM patients compared with controls (0.1 ± 0 AU). Plasma and serum levels of MMP-9 significantly correlated. These results suggest the involvement of MMP-9 in LAM.

Original language	English
Pages (from-to)	124-129
Number of pages	6
Journal	Respiratory Medicine
Volume	103
Issue number	1
DOIs	https://doi.org/10.1016/j.rmed.2008.07.017
Publication status	Published - 2009 Jan
Externally published	Yes

Keywords

Lymphangioleiomyomatosis
MMP-2
MMP-9
Plasma
Serum

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine

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10.1016/j.rmed.2008.07.017

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title = "Matrix metalloproteinases in blood from patients with LAM",

abstract = "Pulmonary lymphangioleiomyomatosis (LAM) is characterized by the proliferation of abnormal smooth muscle cells (LAM cells) and destruction of alveolar structure. Immunohistochemical studies suggest that excess matrix metalloproteinases (MMPs) synthesized by LAM cells function in the proteolytic mechanisms of this disease. We postulated MMP levels in the blood are elevated in LAM patients. Serum samples were collected from 36 LAM patients and 25 controls, and gelatinolytic activities were semi-quantified by gelatin zymography. The reliability of serum data for MMP-9 was confirmed by the measurement of MMP-9 concentration in plasma by enzyme-linked immunosorbent assay as well as by gelatin zymography. Serum levels of MMP-9 (0.7 ± 0.1 AU), but not MMP-2, were significantly elevated in LAM patients compared with controls (0.1 ± 0 AU). Plasma and serum levels of MMP-9 significantly correlated. These results suggest the involvement of MMP-9 in LAM.",

keywords = "Lymphangioleiomyomatosis, MMP-2, MMP-9, Plasma, Serum",

author = "Nao Odajima and Tomoko Betsuyaku and Yasuyuki Nasuhara and Hiromasa Inoue and Kuniaki Seyama and Masaharu Nishimura",

note = "Funding Information: This study was partly funded by scientific research grants from the Ministry of Health, Labor, and Welfare of Japan to the Respiratory Failure Research Group. ",

year = "2009",

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doi = "10.1016/j.rmed.2008.07.017",

language = "English",

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AU - Odajima, Nao

AU - Betsuyaku, Tomoko

AU - Nasuhara, Yasuyuki

AU - Inoue, Hiromasa

AU - Seyama, Kuniaki

AU - Nishimura, Masaharu

N1 - Funding Information: This study was partly funded by scientific research grants from the Ministry of Health, Labor, and Welfare of Japan to the Respiratory Failure Research Group.

PY - 2009/1

Y1 - 2009/1

N2 - Pulmonary lymphangioleiomyomatosis (LAM) is characterized by the proliferation of abnormal smooth muscle cells (LAM cells) and destruction of alveolar structure. Immunohistochemical studies suggest that excess matrix metalloproteinases (MMPs) synthesized by LAM cells function in the proteolytic mechanisms of this disease. We postulated MMP levels in the blood are elevated in LAM patients. Serum samples were collected from 36 LAM patients and 25 controls, and gelatinolytic activities were semi-quantified by gelatin zymography. The reliability of serum data for MMP-9 was confirmed by the measurement of MMP-9 concentration in plasma by enzyme-linked immunosorbent assay as well as by gelatin zymography. Serum levels of MMP-9 (0.7 ± 0.1 AU), but not MMP-2, were significantly elevated in LAM patients compared with controls (0.1 ± 0 AU). Plasma and serum levels of MMP-9 significantly correlated. These results suggest the involvement of MMP-9 in LAM.

AB - Pulmonary lymphangioleiomyomatosis (LAM) is characterized by the proliferation of abnormal smooth muscle cells (LAM cells) and destruction of alveolar structure. Immunohistochemical studies suggest that excess matrix metalloproteinases (MMPs) synthesized by LAM cells function in the proteolytic mechanisms of this disease. We postulated MMP levels in the blood are elevated in LAM patients. Serum samples were collected from 36 LAM patients and 25 controls, and gelatinolytic activities were semi-quantified by gelatin zymography. The reliability of serum data for MMP-9 was confirmed by the measurement of MMP-9 concentration in plasma by enzyme-linked immunosorbent assay as well as by gelatin zymography. Serum levels of MMP-9 (0.7 ± 0.1 AU), but not MMP-2, were significantly elevated in LAM patients compared with controls (0.1 ± 0 AU). Plasma and serum levels of MMP-9 significantly correlated. These results suggest the involvement of MMP-9 in LAM.

KW - Lymphangioleiomyomatosis

KW - MMP-2

KW - MMP-9

KW - Plasma

KW - Serum

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Matrix metalloproteinases in blood from patients with LAM

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Keywords

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