Measurement for optical properties of mitochondria in vitro

Akio Nakajima, Miya Yoshikawa, Tsunenori Arai, Yuji M.D. Morimoto, Makoto Kikuchi, et.al.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The effectiveness of low-intensity light for clinical therapies is well demonstrated, however, the principles of these therapies are not well understood. Our aim of this study is the investigation of optical properties of mitochondria because it seems to be the most important cellular organella in terms of the light-cell interaction. We measured the optical absorption characteristics of an isolated mitochondrial suspension in the range of 300 to 900 nm by means of the transmission methods with an integral sphere. Mitochondrial specific absorption coefficients (ml/g/cm-1) were carried out by the transmission spectrum. The specific absorption coefficient at the maximum absorption of cytochrome cγ peaked about 560 ml/g/cm-1 at 418 nm. Many papers described that the effect of the low intensity light to the biological tissue would be drastically changed by the irradiation conditions. We could calculate a deposit energy of irradiated light in a mitochondrion from our results. We think our results may be useful to describe the quantitative explanation in energy balance for the interaction between the light and mitochondria.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Society of Photo-Optical Instrumentation Engineers
Pages62-67
Number of pages6
ISBN (Print)0819411108
Publication statusPublished - 1993 Jan 1
EventLow-Energy Laser Effects on Biological Systems - Los Angeles, CA, USA
Duration: 1993 Jan 191993 Jan 20

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1883
ISSN (Print)0277-786X

Other

OtherLow-Energy Laser Effects on Biological Systems
CityLos Angeles, CA, USA
Period93/1/1993/1/20

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Nakajima, A., Yoshikawa, M., Arai, T., Morimoto, Y. M. D., Kikuchi, M., & et.al. (1993). Measurement for optical properties of mitochondria in vitro. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 62-67). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1883). Publ by Society of Photo-Optical Instrumentation Engineers.