Measurement for optical properties of mitochondria in vitro

Akio Nakajima, Miya Yoshikawa, Tsunenori Arai, Yuji M D Morimoto, Makoto Kikuchi, et.al.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The effectiveness of low-intensity light for clinical therapies is well demonstrated, however, the principles of these therapies are not well understood. Our aim of this study is the investigation of optical properties of mitochondria because it seems to be the most important cellular organella in terms of the light-cell interaction. We measured the optical absorption characteristics of an isolated mitochondrial suspension in the range of 300 to 900 nm by means of the transmission methods with an integral sphere. Mitochondrial specific absorption coefficients (ml/g/cm-1) were carried out by the transmission spectrum. The specific absorption coefficient at the maximum absorption of cytochrome cγ peaked about 560 ml/g/cm-1 at 418 nm. Many papers described that the effect of the low intensity light to the biological tissue would be drastically changed by the irradiation conditions. We could calculate a deposit energy of irradiated light in a mitochondrion from our results. We think our results may be useful to describe the quantitative explanation in energy balance for the interaction between the light and mitochondria.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Society of Photo-Optical Instrumentation Engineers
Pages62-67
Number of pages6
Volume1883
ISBN (Print)0819411108
Publication statusPublished - 1993
EventLow-Energy Laser Effects on Biological Systems - Los Angeles, CA, USA
Duration: 1993 Jan 191993 Jan 20

Other

OtherLow-Energy Laser Effects on Biological Systems
CityLos Angeles, CA, USA
Period93/1/1993/1/20

Fingerprint

Mitochondria
mitochondria
Optical properties
optical properties
luminous intensity
therapy
absorptivity
cytochromes
optical absorption
deposits
interactions
Energy balance
irradiation
energy
Light absorption
cells
Deposits
Irradiation
Tissue
Proteins

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Nakajima, A., Yoshikawa, M., Arai, T., Morimoto, Y. M. D., Kikuchi, M., & et.al. (1993). Measurement for optical properties of mitochondria in vitro. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1883, pp. 62-67). Publ by Society of Photo-Optical Instrumentation Engineers.

Measurement for optical properties of mitochondria in vitro. / Nakajima, Akio; Yoshikawa, Miya; Arai, Tsunenori; Morimoto, Yuji M D; Kikuchi, Makoto; et.al.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1883 Publ by Society of Photo-Optical Instrumentation Engineers, 1993. p. 62-67.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nakajima, A, Yoshikawa, M, Arai, T, Morimoto, YMD, Kikuchi, M & et.al. 1993, Measurement for optical properties of mitochondria in vitro. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 1883, Publ by Society of Photo-Optical Instrumentation Engineers, pp. 62-67, Low-Energy Laser Effects on Biological Systems, Los Angeles, CA, USA, 93/1/19.
Nakajima A, Yoshikawa M, Arai T, Morimoto YMD, Kikuchi M, et.al. Measurement for optical properties of mitochondria in vitro. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1883. Publ by Society of Photo-Optical Instrumentation Engineers. 1993. p. 62-67
Nakajima, Akio ; Yoshikawa, Miya ; Arai, Tsunenori ; Morimoto, Yuji M D ; Kikuchi, Makoto ; et.al. / Measurement for optical properties of mitochondria in vitro. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1883 Publ by Society of Photo-Optical Instrumentation Engineers, 1993. pp. 62-67
@inproceedings{03f87a3332bb478cbb6368ddb40a21dc,
title = "Measurement for optical properties of mitochondria in vitro",
abstract = "The effectiveness of low-intensity light for clinical therapies is well demonstrated, however, the principles of these therapies are not well understood. Our aim of this study is the investigation of optical properties of mitochondria because it seems to be the most important cellular organella in terms of the light-cell interaction. We measured the optical absorption characteristics of an isolated mitochondrial suspension in the range of 300 to 900 nm by means of the transmission methods with an integral sphere. Mitochondrial specific absorption coefficients (ml/g/cm-1) were carried out by the transmission spectrum. The specific absorption coefficient at the maximum absorption of cytochrome cγ peaked about 560 ml/g/cm-1 at 418 nm. Many papers described that the effect of the low intensity light to the biological tissue would be drastically changed by the irradiation conditions. We could calculate a deposit energy of irradiated light in a mitochondrion from our results. We think our results may be useful to describe the quantitative explanation in energy balance for the interaction between the light and mitochondria.",
author = "Akio Nakajima and Miya Yoshikawa and Tsunenori Arai and Morimoto, {Yuji M D} and Makoto Kikuchi and et.al.",
year = "1993",
language = "English",
isbn = "0819411108",
volume = "1883",
pages = "62--67",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "Publ by Society of Photo-Optical Instrumentation Engineers",

}

TY - GEN

T1 - Measurement for optical properties of mitochondria in vitro

AU - Nakajima, Akio

AU - Yoshikawa, Miya

AU - Arai, Tsunenori

AU - Morimoto, Yuji M D

AU - Kikuchi, Makoto

AU - et.al.,

PY - 1993

Y1 - 1993

N2 - The effectiveness of low-intensity light for clinical therapies is well demonstrated, however, the principles of these therapies are not well understood. Our aim of this study is the investigation of optical properties of mitochondria because it seems to be the most important cellular organella in terms of the light-cell interaction. We measured the optical absorption characteristics of an isolated mitochondrial suspension in the range of 300 to 900 nm by means of the transmission methods with an integral sphere. Mitochondrial specific absorption coefficients (ml/g/cm-1) were carried out by the transmission spectrum. The specific absorption coefficient at the maximum absorption of cytochrome cγ peaked about 560 ml/g/cm-1 at 418 nm. Many papers described that the effect of the low intensity light to the biological tissue would be drastically changed by the irradiation conditions. We could calculate a deposit energy of irradiated light in a mitochondrion from our results. We think our results may be useful to describe the quantitative explanation in energy balance for the interaction between the light and mitochondria.

AB - The effectiveness of low-intensity light for clinical therapies is well demonstrated, however, the principles of these therapies are not well understood. Our aim of this study is the investigation of optical properties of mitochondria because it seems to be the most important cellular organella in terms of the light-cell interaction. We measured the optical absorption characteristics of an isolated mitochondrial suspension in the range of 300 to 900 nm by means of the transmission methods with an integral sphere. Mitochondrial specific absorption coefficients (ml/g/cm-1) were carried out by the transmission spectrum. The specific absorption coefficient at the maximum absorption of cytochrome cγ peaked about 560 ml/g/cm-1 at 418 nm. Many papers described that the effect of the low intensity light to the biological tissue would be drastically changed by the irradiation conditions. We could calculate a deposit energy of irradiated light in a mitochondrion from our results. We think our results may be useful to describe the quantitative explanation in energy balance for the interaction between the light and mitochondria.

UR - http://www.scopus.com/inward/record.url?scp=0027277796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027277796&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:0027277796

SN - 0819411108

VL - 1883

SP - 62

EP - 67

BT - Proceedings of SPIE - The International Society for Optical Engineering

PB - Publ by Society of Photo-Optical Instrumentation Engineers

ER -