TY - GEN
T1 - Measurement for optical properties of mitochondria in vitro
AU - Nakajima, Akio
AU - Yoshikawa, Miya
AU - Arai, Tsunenori
AU - Morimoto, Yuji M.D.
AU - Kikuchi, Makoto
AU - et.al.,
PY - 1993
Y1 - 1993
N2 - The effectiveness of low-intensity light for clinical therapies is well demonstrated, however, the principles of these therapies are not well understood. Our aim of this study is the investigation of optical properties of mitochondria because it seems to be the most important cellular organella in terms of the light-cell interaction. We measured the optical absorption characteristics of an isolated mitochondrial suspension in the range of 300 to 900 nm by means of the transmission methods with an integral sphere. Mitochondrial specific absorption coefficients (ml/g/cm-1) were carried out by the transmission spectrum. The specific absorption coefficient at the maximum absorption of cytochrome cγ peaked about 560 ml/g/cm-1 at 418 nm. Many papers described that the effect of the low intensity light to the biological tissue would be drastically changed by the irradiation conditions. We could calculate a deposit energy of irradiated light in a mitochondrion from our results. We think our results may be useful to describe the quantitative explanation in energy balance for the interaction between the light and mitochondria.
AB - The effectiveness of low-intensity light for clinical therapies is well demonstrated, however, the principles of these therapies are not well understood. Our aim of this study is the investigation of optical properties of mitochondria because it seems to be the most important cellular organella in terms of the light-cell interaction. We measured the optical absorption characteristics of an isolated mitochondrial suspension in the range of 300 to 900 nm by means of the transmission methods with an integral sphere. Mitochondrial specific absorption coefficients (ml/g/cm-1) were carried out by the transmission spectrum. The specific absorption coefficient at the maximum absorption of cytochrome cγ peaked about 560 ml/g/cm-1 at 418 nm. Many papers described that the effect of the low intensity light to the biological tissue would be drastically changed by the irradiation conditions. We could calculate a deposit energy of irradiated light in a mitochondrion from our results. We think our results may be useful to describe the quantitative explanation in energy balance for the interaction between the light and mitochondria.
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M3 - Conference contribution
AN - SCOPUS:0027277796
SN - 0819411108
T3 - Proceedings of SPIE - The International Society for Optical Engineering
SP - 62
EP - 67
BT - Proceedings of SPIE - The International Society for Optical Engineering
PB - Publ by Society of Photo-Optical Instrumentation Engineers
T2 - Low-Energy Laser Effects on Biological Systems
Y2 - 19 January 1993 through 20 January 1993
ER -