Measurement of a stimulatory protein of RNA polymerase II in various mouse organs by the complement fixation test

Hiroomi Tamura, Kazuhisa Sekimizu, Masuo Obinata, Shunji Natori

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The amount of S-II, a protein specifically stimulating RNA polymerase II, was measured in various mouse organs by a micro complement fixation assay. The amount was almost the same in brain, liver, kidney, spleen, and Ehrlich ascites tumor cells on the basis of DNA, being about 3.8 μg/mg DNA, which corresponds to 3.6 × 105 molecules/cell. However, the amount of S-II decreased greatly during erythro-differentiation of Friend leukemia cells and no S-II was detected in matured erythrocytes.

Original languageEnglish
Pages (from-to)1475-1480
Number of pages6
JournalJournal of Biochemistry
Volume88
Issue number5
Publication statusPublished - 1980 Oct
Externally publishedYes

Fingerprint

Complement Fixation Tests
RNA Polymerase II
Fixation
RNA
Mouse
DNA
Complement
Proteins
Protein
Cell
Liver
Tumors
Ehrlich Tumor Carcinoma
Assays
Brain
Erythrocyte
Leukemia
Cells
Kidney
Molecules

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Measurement of a stimulatory protein of RNA polymerase II in various mouse organs by the complement fixation test. / Tamura, Hiroomi; Sekimizu, Kazuhisa; Obinata, Masuo; Natori, Shunji.

In: Journal of Biochemistry, Vol. 88, No. 5, 10.1980, p. 1475-1480.

Research output: Contribution to journalArticle

Tamura, Hiroomi ; Sekimizu, Kazuhisa ; Obinata, Masuo ; Natori, Shunji. / Measurement of a stimulatory protein of RNA polymerase II in various mouse organs by the complement fixation test. In: Journal of Biochemistry. 1980 ; Vol. 88, No. 5. pp. 1475-1480.
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