Measurement of free insulin—like growth factor—i using immunoradiometric assay

Makoto Takada, Hiroyuki Nakanome, Mariko Kishida, Sachio Hirose, Tomonobu Hasegawa, Yukihiro Hasegawa

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The free Insulin-like growth factor-I (IGF-I) in plasma from normal adults was directly measured with a newly developed highly sensitive immunoradiometric assay (IRMA) for IGF-I. The capture antibody did not crossreact with IGF-I associated binding proteins which exist in plasma, and the assay was designed not to shift the equilibrium of the IGF-I and binding proteins. Total IGF-I concentration was measured using this assay with preliminary acid—ethanol extraction. Approximately 1 percent of total IGF-I existed in the free form. Gel filtration of plasma was also used to separate the free IGF-I from its bound form. The free/total ratio of IGF-I as determined by gel filtration was similar to that determined directly by IRMA with and without acid—ethanol extraction.

Original languageEnglish
Pages (from-to)263-276
Number of pages14
JournalJournal of Immunoassay
Volume15
Issue number3
DOIs
Publication statusPublished - 1994 Aug 1
Externally publishedYes

Fingerprint

Immunoradiometric Assay
Insulin-Like Growth Factor I
Assays
Growth
Plasmas
Gel Chromatography
Carrier Proteins
Gels
Insulin-Like Growth Factor Binding Proteins
Antibodies

Keywords

  • form binding protein
  • immunoradiometric assay Western immunoblot)
  • insulin
  • like growth factor-I free

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Measurement of free insulin—like growth factor—i using immunoradiometric assay. / Takada, Makoto; Nakanome, Hiroyuki; Kishida, Mariko; Hirose, Sachio; Hasegawa, Tomonobu; Hasegawa, Yukihiro.

In: Journal of Immunoassay, Vol. 15, No. 3, 01.08.1994, p. 263-276.

Research output: Contribution to journalArticle

Takada, Makoto ; Nakanome, Hiroyuki ; Kishida, Mariko ; Hirose, Sachio ; Hasegawa, Tomonobu ; Hasegawa, Yukihiro. / Measurement of free insulin—like growth factor—i using immunoradiometric assay. In: Journal of Immunoassay. 1994 ; Vol. 15, No. 3. pp. 263-276.
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