Measurement of Insulin-Like Growth Factor Binding Protein-3 Proteolytic Activity with Western Immunoblot in Acute Viral Diseases in Childhood

Taiji Aso, Tomonobu Hasegawa, Yukihiro Hasegawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Insulin-like growth factor binding protein-3 (IGFBP-3) is a main binding protein for IGF-I and II (IGFs). During pregnancy IGFBP-3 proteolytic activity has been found in sera and it may result in an increase in IGFs bioavailability so that the maternal tissues and placenta can grow. The proteolytic activity has also been reported to be induced in catabolic conditions such as after surgery and the subsequent increase in IGFs bioavailability may be important in overcoming catabolic conditions. We have this time established the measurement of the proteolytic activity using western immunoblot. This dose not use radioabeled IGFBP-3 and takes less time than the previous method. With the new method we measured the proteolytic activity in children with acute viral disease. Significant results of IGFBP-3 proteolytic activity were obtained from 2 patients with measles, suggesting IGF-I and II might also be physiologically important in those patients.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalClinical Pediatric Endocrinology
Volume3
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor Binding Protein 3
Acute Disease
Virus Diseases
Western Blotting
Insulin-Like Growth Factor II
Insulin-Like Growth Factor I
Biological Availability
Measles
Placenta
Carrier Proteins
Mothers
Pregnancy
Serum

Keywords

  • IGFBP-3
  • IGFBP-3 proteolytic activity
  • IGFs bioavailability
  • western immunoblot

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

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abstract = "Insulin-like growth factor binding protein-3 (IGFBP-3) is a main binding protein for IGF-I and II (IGFs). During pregnancy IGFBP-3 proteolytic activity has been found in sera and it may result in an increase in IGFs bioavailability so that the maternal tissues and placenta can grow. The proteolytic activity has also been reported to be induced in catabolic conditions such as after surgery and the subsequent increase in IGFs bioavailability may be important in overcoming catabolic conditions. We have this time established the measurement of the proteolytic activity using western immunoblot. This dose not use radioabeled IGFBP-3 and takes less time than the previous method. With the new method we measured the proteolytic activity in children with acute viral disease. Significant results of IGFBP-3 proteolytic activity were obtained from 2 patients with measles, suggesting IGF-I and II might also be physiologically important in those patients.",
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AU - Hasegawa, Yukihiro

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