Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent

Takamitsu Nakano, Yoshiharu Tokita, Takeaki Nagamine, Akira Tanaka, Mitsuyo Okazaki, Shinichi Yatsuzuka, Noriko Tamei, Younosuke Shimomura, Yoshitaka Ando, Suguru Akamatsu, Masaki Q Fujita, Katsuyuki Nakajima

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Serum concentration of remnant-like lipoprotein particles (RLP) have been measured by cholesterol as RLP-C for clinical diagnostic purpose. However, the measurement of TG in RLP and the ratio of RLP-TG/total TG has not been well established. Method: Highly sensitive triglyceride assay reagent (TG-EX) was used for RLP-TG assay and compared with the previously used TG reagent (Determiner LTGII). Sera in health check-up populations, cardiovascular disease, diabetes and oral fat load cases were used for the evaluation of the new RLP-TG assay. Serum TC, TG, HDL-C, LDL-C and RLP-C concentrations were also determined in above cases. Results: The detection limit of new RLP-TG using TG-EX was 2.0. mg/dl. The within-run imprecision (n = 10) was CV = 3.0% (RLP-TG: 4.1. mg. ±. 0.7. mg/dl), CV = 1.4% (RLP-TG: 42.0. ±. 0.6. mg/dl) and CV = 0.5% (RLP-TG: 100.6. ±. 0.6. mg/dl). Cut-off value (75 percentile) of RLP-TG determined in the fasting Japanese population was 13.1. mg/dl in men and 9.9. mg/dl in women. In patients with metabolic syndrome, cardiovascular disease and diabetes, RLP-TG levels were significantly higher than those in normal control subjects. RLP-TG levels increased significantly after an oral fat load and the ratio of RLP-TG/total TG increased. >. 3-fold compared to the ratio in the fasting state. Approximately 80% of TG increased after an oral fat load was TG derived from remnant lipoproteins. Conclusion: Normal range of plasma RLP-TG in the fasting Japanese population was first determined using a highly sensitive TG assay reagent. RLP-TG was shown to be higher in cases with metabolic syndrome, cardiovascular disease, etc and a better marker than RLP-C for the measurement of postprandial remnant lipoproteins, together with total TG for RLP-TG/total TG ratio.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalClinica Chimica Acta
Volume412
Issue number1-2
DOIs
Publication statusPublished - 2011 Jan 14

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Lipoproteins
Assays
Triglycerides
Serum
Fasting
Cardiovascular Diseases
Fats
Medical problems
Population

Keywords

  • Metabolic syndrome
  • Postprandial hyperlipidemia
  • Remnant-like lipoprotein particles (RLP)
  • RLP-cholesterol (RLP-C)
  • RLP-TG/total TG ratio
  • RLP-triglyceride (RLP-TG)

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent. / Nakano, Takamitsu; Tokita, Yoshiharu; Nagamine, Takeaki; Tanaka, Akira; Okazaki, Mitsuyo; Yatsuzuka, Shinichi; Tamei, Noriko; Shimomura, Younosuke; Ando, Yoshitaka; Akamatsu, Suguru; Fujita, Masaki Q; Nakajima, Katsuyuki.

In: Clinica Chimica Acta, Vol. 412, No. 1-2, 14.01.2011, p. 71-78.

Research output: Contribution to journalArticle

Nakano, T, Tokita, Y, Nagamine, T, Tanaka, A, Okazaki, M, Yatsuzuka, S, Tamei, N, Shimomura, Y, Ando, Y, Akamatsu, S, Fujita, MQ & Nakajima, K 2011, 'Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent', Clinica Chimica Acta, vol. 412, no. 1-2, pp. 71-78. https://doi.org/10.1016/j.cca.2010.09.040
Nakano, Takamitsu ; Tokita, Yoshiharu ; Nagamine, Takeaki ; Tanaka, Akira ; Okazaki, Mitsuyo ; Yatsuzuka, Shinichi ; Tamei, Noriko ; Shimomura, Younosuke ; Ando, Yoshitaka ; Akamatsu, Suguru ; Fujita, Masaki Q ; Nakajima, Katsuyuki. / Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent. In: Clinica Chimica Acta. 2011 ; Vol. 412, No. 1-2. pp. 71-78.
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T1 - Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent

AU - Nakano, Takamitsu

AU - Tokita, Yoshiharu

AU - Nagamine, Takeaki

AU - Tanaka, Akira

AU - Okazaki, Mitsuyo

AU - Yatsuzuka, Shinichi

AU - Tamei, Noriko

AU - Shimomura, Younosuke

AU - Ando, Yoshitaka

AU - Akamatsu, Suguru

AU - Fujita, Masaki Q

AU - Nakajima, Katsuyuki

PY - 2011/1/14

Y1 - 2011/1/14

N2 - Background: Serum concentration of remnant-like lipoprotein particles (RLP) have been measured by cholesterol as RLP-C for clinical diagnostic purpose. However, the measurement of TG in RLP and the ratio of RLP-TG/total TG has not been well established. Method: Highly sensitive triglyceride assay reagent (TG-EX) was used for RLP-TG assay and compared with the previously used TG reagent (Determiner LTGII). Sera in health check-up populations, cardiovascular disease, diabetes and oral fat load cases were used for the evaluation of the new RLP-TG assay. Serum TC, TG, HDL-C, LDL-C and RLP-C concentrations were also determined in above cases. Results: The detection limit of new RLP-TG using TG-EX was 2.0. mg/dl. The within-run imprecision (n = 10) was CV = 3.0% (RLP-TG: 4.1. mg. ±. 0.7. mg/dl), CV = 1.4% (RLP-TG: 42.0. ±. 0.6. mg/dl) and CV = 0.5% (RLP-TG: 100.6. ±. 0.6. mg/dl). Cut-off value (75 percentile) of RLP-TG determined in the fasting Japanese population was 13.1. mg/dl in men and 9.9. mg/dl in women. In patients with metabolic syndrome, cardiovascular disease and diabetes, RLP-TG levels were significantly higher than those in normal control subjects. RLP-TG levels increased significantly after an oral fat load and the ratio of RLP-TG/total TG increased. >. 3-fold compared to the ratio in the fasting state. Approximately 80% of TG increased after an oral fat load was TG derived from remnant lipoproteins. Conclusion: Normal range of plasma RLP-TG in the fasting Japanese population was first determined using a highly sensitive TG assay reagent. RLP-TG was shown to be higher in cases with metabolic syndrome, cardiovascular disease, etc and a better marker than RLP-C for the measurement of postprandial remnant lipoproteins, together with total TG for RLP-TG/total TG ratio.

AB - Background: Serum concentration of remnant-like lipoprotein particles (RLP) have been measured by cholesterol as RLP-C for clinical diagnostic purpose. However, the measurement of TG in RLP and the ratio of RLP-TG/total TG has not been well established. Method: Highly sensitive triglyceride assay reagent (TG-EX) was used for RLP-TG assay and compared with the previously used TG reagent (Determiner LTGII). Sera in health check-up populations, cardiovascular disease, diabetes and oral fat load cases were used for the evaluation of the new RLP-TG assay. Serum TC, TG, HDL-C, LDL-C and RLP-C concentrations were also determined in above cases. Results: The detection limit of new RLP-TG using TG-EX was 2.0. mg/dl. The within-run imprecision (n = 10) was CV = 3.0% (RLP-TG: 4.1. mg. ±. 0.7. mg/dl), CV = 1.4% (RLP-TG: 42.0. ±. 0.6. mg/dl) and CV = 0.5% (RLP-TG: 100.6. ±. 0.6. mg/dl). Cut-off value (75 percentile) of RLP-TG determined in the fasting Japanese population was 13.1. mg/dl in men and 9.9. mg/dl in women. In patients with metabolic syndrome, cardiovascular disease and diabetes, RLP-TG levels were significantly higher than those in normal control subjects. RLP-TG levels increased significantly after an oral fat load and the ratio of RLP-TG/total TG increased. >. 3-fold compared to the ratio in the fasting state. Approximately 80% of TG increased after an oral fat load was TG derived from remnant lipoproteins. Conclusion: Normal range of plasma RLP-TG in the fasting Japanese population was first determined using a highly sensitive TG assay reagent. RLP-TG was shown to be higher in cases with metabolic syndrome, cardiovascular disease, etc and a better marker than RLP-C for the measurement of postprandial remnant lipoproteins, together with total TG for RLP-TG/total TG ratio.

KW - Metabolic syndrome

KW - Postprandial hyperlipidemia

KW - Remnant-like lipoprotein particles (RLP)

KW - RLP-cholesterol (RLP-C)

KW - RLP-TG/total TG ratio

KW - RLP-triglyceride (RLP-TG)

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