TY - JOUR
T1 - Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent
AU - Nakano, Takamitsu
AU - Tokita, Yoshiharu
AU - Nagamine, Takeaki
AU - Tanaka, Akira
AU - Okazaki, Mitsuyo
AU - Yatsuzuka, Shinichi
AU - Tamei, Noriko
AU - Shimomura, Younosuke
AU - Ando, Yoshitaka
AU - Akamatsu, Suguru
AU - Fujita, Masaki Q.
AU - Nakajima, Katsuyuki
N1 - Funding Information:
This work is supported in part by Grant-in-Aid from Japan Society for the Promotion of Science (JSPS) , No. 20406020 . Authors greatly appreciate Dr. Ito and Ms Hirao in Denka Seiken Co., Ltd. for providing the highly sensitive TG assay reagents.
PY - 2011/1/14
Y1 - 2011/1/14
N2 - Background: Serum concentration of remnant-like lipoprotein particles (RLP) have been measured by cholesterol as RLP-C for clinical diagnostic purpose. However, the measurement of TG in RLP and the ratio of RLP-TG/total TG has not been well established. Method: Highly sensitive triglyceride assay reagent (TG-EX) was used for RLP-TG assay and compared with the previously used TG reagent (Determiner LTGII). Sera in health check-up populations, cardiovascular disease, diabetes and oral fat load cases were used for the evaluation of the new RLP-TG assay. Serum TC, TG, HDL-C, LDL-C and RLP-C concentrations were also determined in above cases. Results: The detection limit of new RLP-TG using TG-EX was 2.0. mg/dl. The within-run imprecision (n = 10) was CV = 3.0% (RLP-TG: 4.1. mg. ±. 0.7. mg/dl), CV = 1.4% (RLP-TG: 42.0. ±. 0.6. mg/dl) and CV = 0.5% (RLP-TG: 100.6. ±. 0.6. mg/dl). Cut-off value (75 percentile) of RLP-TG determined in the fasting Japanese population was 13.1. mg/dl in men and 9.9. mg/dl in women. In patients with metabolic syndrome, cardiovascular disease and diabetes, RLP-TG levels were significantly higher than those in normal control subjects. RLP-TG levels increased significantly after an oral fat load and the ratio of RLP-TG/total TG increased. >. 3-fold compared to the ratio in the fasting state. Approximately 80% of TG increased after an oral fat load was TG derived from remnant lipoproteins. Conclusion: Normal range of plasma RLP-TG in the fasting Japanese population was first determined using a highly sensitive TG assay reagent. RLP-TG was shown to be higher in cases with metabolic syndrome, cardiovascular disease, etc and a better marker than RLP-C for the measurement of postprandial remnant lipoproteins, together with total TG for RLP-TG/total TG ratio.
AB - Background: Serum concentration of remnant-like lipoprotein particles (RLP) have been measured by cholesterol as RLP-C for clinical diagnostic purpose. However, the measurement of TG in RLP and the ratio of RLP-TG/total TG has not been well established. Method: Highly sensitive triglyceride assay reagent (TG-EX) was used for RLP-TG assay and compared with the previously used TG reagent (Determiner LTGII). Sera in health check-up populations, cardiovascular disease, diabetes and oral fat load cases were used for the evaluation of the new RLP-TG assay. Serum TC, TG, HDL-C, LDL-C and RLP-C concentrations were also determined in above cases. Results: The detection limit of new RLP-TG using TG-EX was 2.0. mg/dl. The within-run imprecision (n = 10) was CV = 3.0% (RLP-TG: 4.1. mg. ±. 0.7. mg/dl), CV = 1.4% (RLP-TG: 42.0. ±. 0.6. mg/dl) and CV = 0.5% (RLP-TG: 100.6. ±. 0.6. mg/dl). Cut-off value (75 percentile) of RLP-TG determined in the fasting Japanese population was 13.1. mg/dl in men and 9.9. mg/dl in women. In patients with metabolic syndrome, cardiovascular disease and diabetes, RLP-TG levels were significantly higher than those in normal control subjects. RLP-TG levels increased significantly after an oral fat load and the ratio of RLP-TG/total TG increased. >. 3-fold compared to the ratio in the fasting state. Approximately 80% of TG increased after an oral fat load was TG derived from remnant lipoproteins. Conclusion: Normal range of plasma RLP-TG in the fasting Japanese population was first determined using a highly sensitive TG assay reagent. RLP-TG was shown to be higher in cases with metabolic syndrome, cardiovascular disease, etc and a better marker than RLP-C for the measurement of postprandial remnant lipoproteins, together with total TG for RLP-TG/total TG ratio.
KW - Metabolic syndrome
KW - Postprandial hyperlipidemia
KW - RLP-TG/total TG ratio
KW - RLP-cholesterol (RLP-C)
KW - RLP-triglyceride (RLP-TG)
KW - Remnant-like lipoprotein particles (RLP)
UR - http://www.scopus.com/inward/record.url?scp=78650512673&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650512673&partnerID=8YFLogxK
U2 - 10.1016/j.cca.2010.09.040
DO - 10.1016/j.cca.2010.09.040
M3 - Article
C2 - 20951691
AN - SCOPUS:78650512673
SN - 0009-8981
VL - 412
SP - 71
EP - 78
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1-2
ER -
