Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent

Takamitsu Nakano, Yoshiharu Tokita, Takeaki Nagamine, Akira Tanaka, Mitsuyo Okazaki, Shinichi Yatsuzuka, Noriko Tamei, Younosuke Shimomura, Yoshitaka Ando, Suguru Akamatsu, Masaki Q. Fujita, Katsuyuki Nakajima

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12 Citations (Scopus)

Abstract

Background: Serum concentration of remnant-like lipoprotein particles (RLP) have been measured by cholesterol as RLP-C for clinical diagnostic purpose. However, the measurement of TG in RLP and the ratio of RLP-TG/total TG has not been well established. Method: Highly sensitive triglyceride assay reagent (TG-EX) was used for RLP-TG assay and compared with the previously used TG reagent (Determiner LTGII). Sera in health check-up populations, cardiovascular disease, diabetes and oral fat load cases were used for the evaluation of the new RLP-TG assay. Serum TC, TG, HDL-C, LDL-C and RLP-C concentrations were also determined in above cases. Results: The detection limit of new RLP-TG using TG-EX was 2.0. mg/dl. The within-run imprecision (n = 10) was CV = 3.0% (RLP-TG: 4.1. mg. ±. 0.7. mg/dl), CV = 1.4% (RLP-TG: 42.0. ±. 0.6. mg/dl) and CV = 0.5% (RLP-TG: 100.6. ±. 0.6. mg/dl). Cut-off value (75 percentile) of RLP-TG determined in the fasting Japanese population was 13.1. mg/dl in men and 9.9. mg/dl in women. In patients with metabolic syndrome, cardiovascular disease and diabetes, RLP-TG levels were significantly higher than those in normal control subjects. RLP-TG levels increased significantly after an oral fat load and the ratio of RLP-TG/total TG increased. >. 3-fold compared to the ratio in the fasting state. Approximately 80% of TG increased after an oral fat load was TG derived from remnant lipoproteins. Conclusion: Normal range of plasma RLP-TG in the fasting Japanese population was first determined using a highly sensitive TG assay reagent. RLP-TG was shown to be higher in cases with metabolic syndrome, cardiovascular disease, etc and a better marker than RLP-C for the measurement of postprandial remnant lipoproteins, together with total TG for RLP-TG/total TG ratio.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalClinica Chimica Acta
Volume412
Issue number1-2
DOIs
Publication statusPublished - 2011 Jan 14

Keywords

  • Metabolic syndrome
  • Postprandial hyperlipidemia
  • RLP-TG/total TG ratio
  • RLP-cholesterol (RLP-C)
  • RLP-triglyceride (RLP-TG)
  • Remnant-like lipoprotein particles (RLP)

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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    Nakano, T., Tokita, Y., Nagamine, T., Tanaka, A., Okazaki, M., Yatsuzuka, S., Tamei, N., Shimomura, Y., Ando, Y., Akamatsu, S., Fujita, M. Q., & Nakajima, K. (2011). Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent. Clinica Chimica Acta, 412(1-2), 71-78. https://doi.org/10.1016/j.cca.2010.09.040