Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy

Hiroshi Hashimoto; Keisuke Isobe; Akira Suda; Fumihiko Kannari; Hiroyuki Kawano; Hideaki Mizuno; Atsushi Miyawaki; Katsumi Midorikawa

doi:10.1364/AO.49.003323

Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy

Hiroshi Hashimoto, Keisuke Isobe, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

Department of Electronics and Electrical Engineering

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

We present measurements of two-photon excitation (TPE) spectra of various fluorescent proteins with nonlinear Fourier-transform spectroscopy. By using an ultrabroadband laser pulse with a spectrum ranging from 700 to 1100 nm, the absolute TPE spectra of six typical fluorescent proteins (SeBFP, Sapphire, eGFP, eCFP, Venus, DsRed) were measured with high spectral resolution.

Original language	English
Pages (from-to)	3323-3329
Number of pages	7
Journal	Applied Optics
Volume	49
Issue number	17
DOIs	https://doi.org/10.1364/AO.49.003323
Publication status	Published - 2010 Jun 10

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

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abstract = "We present measurements of two-photon excitation (TPE) spectra of various fluorescent proteins with nonlinear Fourier-transform spectroscopy. By using an ultrabroadband laser pulse with a spectrum ranging from 700 to 1100 nm, the absolute TPE spectra of six typical fluorescent proteins (SeBFP, Sapphire, eGFP, eCFP, Venus, DsRed) were measured with high spectral resolution.",

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AU - Hashimoto, Hiroshi

AU - Isobe, Keisuke

AU - Suda, Akira

AU - Kannari, Fumihiko

AU - Kawano, Hiroyuki

AU - Mizuno, Hideaki

AU - Miyawaki, Atsushi

AU - Midorikawa, Katsumi

PY - 2010/6/10

Y1 - 2010/6/10

N2 - We present measurements of two-photon excitation (TPE) spectra of various fluorescent proteins with nonlinear Fourier-transform spectroscopy. By using an ultrabroadband laser pulse with a spectrum ranging from 700 to 1100 nm, the absolute TPE spectra of six typical fluorescent proteins (SeBFP, Sapphire, eGFP, eCFP, Venus, DsRed) were measured with high spectral resolution.

AB - We present measurements of two-photon excitation (TPE) spectra of various fluorescent proteins with nonlinear Fourier-transform spectroscopy. By using an ultrabroadband laser pulse with a spectrum ranging from 700 to 1100 nm, the absolute TPE spectra of six typical fluorescent proteins (SeBFP, Sapphire, eGFP, eCFP, Venus, DsRed) were measured with high spectral resolution.

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JO - Applied Optics

JF - Applied Optics

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Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy

Abstract

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