Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy

Hiroshi Hashimoto, Keisuke Isobe, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We present measurements of two-photon excitation (TPE) spectra of various fluorescent proteins with nonlinear Fourier-transform spectroscopy. By using an ultrabroadband laser pulse with a spectrum ranging from 700 to 1100 nm, the absolute TPE spectra of six typical fluorescent proteins (SeBFP, Sapphire, eGFP, eCFP, Venus, DsRed) were measured with high spectral resolution.

Original languageEnglish
Pages (from-to)3323-3329
Number of pages7
JournalApplied Optics
Volume49
Issue number17
DOIs
Publication statusPublished - 2010 Jun 10

Fingerprint

Fourier transforms
Photons
Spectroscopy
proteins
Proteins
Spectral resolution
photons
Sapphire
spectroscopy
excitation
Laser pulses
Venus (planet)
spectral resolution
sapphire
high resolution
pulses
lasers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy. / Hashimoto, Hiroshi; Isobe, Keisuke; Suda, Akira; Kannari, Fumihiko; Kawano, Hiroyuki; Mizuno, Hideaki; Miyawaki, Atsushi; Midorikawa, Katsumi.

In: Applied Optics, Vol. 49, No. 17, 10.06.2010, p. 3323-3329.

Research output: Contribution to journalArticle

Hashimoto, H, Isobe, K, Suda, A, Kannari, F, Kawano, H, Mizuno, H, Miyawaki, A & Midorikawa, K 2010, 'Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy', Applied Optics, vol. 49, no. 17, pp. 3323-3329. https://doi.org/10.1364/AO.49.003323
Hashimoto, Hiroshi ; Isobe, Keisuke ; Suda, Akira ; Kannari, Fumihiko ; Kawano, Hiroyuki ; Mizuno, Hideaki ; Miyawaki, Atsushi ; Midorikawa, Katsumi. / Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy. In: Applied Optics. 2010 ; Vol. 49, No. 17. pp. 3323-3329.
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