TY - JOUR
T1 - Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy
AU - Isobe, Keisuke
AU - Hashimoto, Hiroshi
AU - Suda, Akira
AU - Kannari, Fumihiko
AU - Kawano, Hiroyuki
AU - Mizuno, Hideaki
AU - Miyawaki, Atsushi
AU - Midorikawa, Katsumi
N1 - Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2010/9/1
Y1 - 2010/9/1
N2 - We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion is much different to that for green-fluorescence. This is similar to the difference between the one-photon excitation of photoconversion in the neutral form and that of green-fluorescence in the ionized form.
AB - We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion is much different to that for green-fluorescence. This is similar to the difference between the one-photon excitation of photoconversion in the neutral form and that of green-fluorescence in the ionized form.
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U2 - 10.1364/BOE.1.000687
DO - 10.1364/BOE.1.000687
M3 - Article
AN - SCOPUS:82455170934
SN - 2156-7085
VL - 1
SP - 687
EP - 693
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 2
ER -