Mechanical cell contact system by a parylene rail filter for study of cell-cell interaction mediated by connexin gap junction

Y. Abe, K. Kamiya, T. Osaki, R. Kawano, K. Akiyoshi, N. Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper proposes a mechanical cell contact system using a parylene rail filter (PRF) for in vitro analysis of connexin functions in cell-cell interaction. Connexin is a membrane protein that mediates interaction between contacting cells and allows small molecules to migrate between the cells. To study this molecular transfer and connexin function, cells must be maintained contact throughout experiments for days under the microscope. We reported a mechanical single liposome trap system with various advantages in MEMS2013 [1]. In this present study, we developed a mechanical cell trap system that traps cells in contact with each other by using a micropatterned parylene rail. The number of trapped cells could be controlled by the length of the rail and the calcein dye transfer between the neighboring cells via connexin gap junctions (GJs) was successfully observed.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages407-409
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Keywords

  • Cell trap and contact
  • Cell-cell interaction
  • Gap junction
  • Microfluidic device

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Abe, Y., Kamiya, K., Osaki, T., Kawano, R., Akiyoshi, K., Miki, N., & Takeuchi, S. (2013). Mechanical cell contact system by a parylene rail filter for study of cell-cell interaction mediated by connexin gap junction. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 407-409). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.