Mechanical pumpless giant liposome trapping system using parylene micro filter for biological assay

Yuta Abe, K. Kamiya, T. Osaki, R. Kawano, Norihisa Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This paper proposes a non-chemical, mechanical giant liposome trapping method using a parylene filter with micrometer-order-sized holes. Giant liposomes can be useful reaction sites for biological assays, where immobilization of the liposomes is the key protocol for microscopic observation. The proposed mechanical trapping method using the parylene filter and gentle flow does not require pretreatments on the liposomes that are time consuming and costly. In addition, this method does not strongly bind but only traps the liposomes, which allows the liposomes to be chemically treated while being trapped and then, to be released. We demonstrated size-dependent trapping, solution exchange and releasing of liposomes. The proposed trapping method and microfluidic device are readily applicable to highly efficient giant liposome assays.

Original languageEnglish
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages949-950
Number of pages2
DOIs
Publication statusPublished - 2013
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
Duration: 2013 Jan 202013 Jan 24

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
CountryTaiwan, Province of China
CityTaipei
Period13/1/2013/1/24

Fingerprint

Liposomes
Assays
trapping
filters
microfluidic devices
releasing
immobilization
pretreatment
micrometers
traps
parylene
Microfluidics
Ion exchange

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Abe, Y., Kamiya, K., Osaki, T., Kawano, R., Miki, N., & Takeuchi, S. (2013). Mechanical pumpless giant liposome trapping system using parylene micro filter for biological assay. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 949-950). [6474402] https://doi.org/10.1109/MEMSYS.2013.6474402

Mechanical pumpless giant liposome trapping system using parylene micro filter for biological assay. / Abe, Yuta; Kamiya, K.; Osaki, T.; Kawano, R.; Miki, Norihisa; Takeuchi, S.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. p. 949-950 6474402.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abe, Y, Kamiya, K, Osaki, T, Kawano, R, Miki, N & Takeuchi, S 2013, Mechanical pumpless giant liposome trapping system using parylene micro filter for biological assay. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 6474402, pp. 949-950, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, Province of China, 13/1/20. https://doi.org/10.1109/MEMSYS.2013.6474402
Abe Y, Kamiya K, Osaki T, Kawano R, Miki N, Takeuchi S. Mechanical pumpless giant liposome trapping system using parylene micro filter for biological assay. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. p. 949-950. 6474402 https://doi.org/10.1109/MEMSYS.2013.6474402
Abe, Yuta ; Kamiya, K. ; Osaki, T. ; Kawano, R. ; Miki, Norihisa ; Takeuchi, S. / Mechanical pumpless giant liposome trapping system using parylene micro filter for biological assay. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. pp. 949-950
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