Mechanisms of Helicobacter pylori antibiotic resistance and molecular testing

Toshihiro Nishizawa, Hidekazu Suzuki

Research output: Contribution to journalReview article

24 Citations (Scopus)

Abstract

Antibiotic resistance in Helicobacter pylori (H.pylori) is the main factor affecting the efficacy of current treatment methods against infection caused by this organism. The traditional culture methods for testing bacterial susceptibility to antibiotics are expensive and require 10-14 days. Since resistance to clarithromycin, fluoroquinolone, and tetracycline seems to be exclusively caused by specific mutations in a small region of the responsible gene, molecular methods offer an attractive alternative to the above-mentioned techniques. The technique of polymerase chain reaction (PCR) is an accurate and rapid method for the detection of mutations that confer antibiotic resistance. This review highlights the mechanisms of antibiotic resistance in H. pylori and the molecular methods for antibiotic susceptibility testing.

Original languageEnglish
Article number19
JournalFrontiers in Molecular Biosciences
Volume1
Issue numberOCT
DOIs
Publication statusPublished - 2014 Oct 24

Fingerprint

Microbial Drug Resistance
Helicobacter pylori
Anti-Bacterial Agents
Testing
Mutation
Clarithromycin
Polymerase chain reaction
Fluoroquinolones
Tetracycline
Genes
Polymerase Chain Reaction
Infection

Keywords

  • 16S rRNA
  • 23S rRNA
  • GyrA
  • Helicobacter pylori
  • PBP1

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Mechanisms of Helicobacter pylori antibiotic resistance and molecular testing. / Nishizawa, Toshihiro; Suzuki, Hidekazu.

In: Frontiers in Molecular Biosciences, Vol. 1, No. OCT, 19, 24.10.2014.

Research output: Contribution to journalReview article

Nishizawa, Toshihiro ; Suzuki, Hidekazu. / Mechanisms of Helicobacter pylori antibiotic resistance and molecular testing. In: Frontiers in Molecular Biosciences. 2014 ; Vol. 1, No. OCT.
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