Mechanisms of photoinduced cochlear ischemia in the guinea pig

H. Saito, Kaoru Ogawa, Y. Inoue, J. Kanzaki, T. Harada, N. Hoya

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An experimental model of cochlear ischemia using the photosensitization reaction of rose bengal (RB) is reported. Ischemia was induced by the systemic administration of RB solution and localized irradiation of the cochlea with a green light of specific wavelength for RB activation. After the RB solution was administered, the cochlear blood flow was temporarily increased and then decreased, and the latency of wave I of the auditory brainstem response increased and the amplitude of wave I decreased after RB injection and finally wave I disappeared completely. A histological study revealed thrombus formation in the vessels of the irradiated cochlea not only in the stria vascularis and the spiral ligament, but also in the modiolus. L-Histidine, a scavenger of singlet oxygen (1O2) and hydroxyl radical (·OH), significantly prolonged cochlear dysfunction, but D-mannitol, a specific scavenger of ·OH did not, suggesting that 1O2 has an important role in this reaction.

Original languageEnglish
Pages (from-to)148-154
Number of pages7
JournalORL
Volume63
Issue number3
DOIs
Publication statusPublished - 2001 May

Fingerprint

Rose Bengal
Cochlea
Guinea Pigs
Ischemia
Spiral Ligament of Cochlea
Stria Vascularis
Methyl Green
Photosensitivity Disorders
Singlet Oxygen
Brain Stem Auditory Evoked Potentials
Mannitol
Histidine
Hydroxyl Radical
Reactive Oxygen Species
Thrombosis
Theoretical Models
Injections

Keywords

  • Cochlear ischemia
  • Experimental model
  • L-Histidine
  • Photosensitizer
  • Rose bengal
  • Singlet oxygen

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Saito, H., Ogawa, K., Inoue, Y., Kanzaki, J., Harada, T., & Hoya, N. (2001). Mechanisms of photoinduced cochlear ischemia in the guinea pig. ORL, 63(3), 148-154. https://doi.org/10.1159/000055731

Mechanisms of photoinduced cochlear ischemia in the guinea pig. / Saito, H.; Ogawa, Kaoru; Inoue, Y.; Kanzaki, J.; Harada, T.; Hoya, N.

In: ORL, Vol. 63, No. 3, 05.2001, p. 148-154.

Research output: Contribution to journalArticle

Saito, H, Ogawa, K, Inoue, Y, Kanzaki, J, Harada, T & Hoya, N 2001, 'Mechanisms of photoinduced cochlear ischemia in the guinea pig', ORL, vol. 63, no. 3, pp. 148-154. https://doi.org/10.1159/000055731
Saito H, Ogawa K, Inoue Y, Kanzaki J, Harada T, Hoya N. Mechanisms of photoinduced cochlear ischemia in the guinea pig. ORL. 2001 May;63(3):148-154. https://doi.org/10.1159/000055731
Saito, H. ; Ogawa, Kaoru ; Inoue, Y. ; Kanzaki, J. ; Harada, T. ; Hoya, N. / Mechanisms of photoinduced cochlear ischemia in the guinea pig. In: ORL. 2001 ; Vol. 63, No. 3. pp. 148-154.
@article{6428115972b14c5c9905be88b03d76a3,
title = "Mechanisms of photoinduced cochlear ischemia in the guinea pig",
abstract = "An experimental model of cochlear ischemia using the photosensitization reaction of rose bengal (RB) is reported. Ischemia was induced by the systemic administration of RB solution and localized irradiation of the cochlea with a green light of specific wavelength for RB activation. After the RB solution was administered, the cochlear blood flow was temporarily increased and then decreased, and the latency of wave I of the auditory brainstem response increased and the amplitude of wave I decreased after RB injection and finally wave I disappeared completely. A histological study revealed thrombus formation in the vessels of the irradiated cochlea not only in the stria vascularis and the spiral ligament, but also in the modiolus. L-Histidine, a scavenger of singlet oxygen (1O2) and hydroxyl radical (·OH), significantly prolonged cochlear dysfunction, but D-mannitol, a specific scavenger of ·OH did not, suggesting that 1O2 has an important role in this reaction.",
keywords = "Cochlear ischemia, Experimental model, L-Histidine, Photosensitizer, Rose bengal, Singlet oxygen",
author = "H. Saito and Kaoru Ogawa and Y. Inoue and J. Kanzaki and T. Harada and N. Hoya",
year = "2001",
month = "5",
doi = "10.1159/000055731",
language = "English",
volume = "63",
pages = "148--154",
journal = "ORL; journal for oto-rhino-laryngology and its related specialties",
issn = "0301-1569",
publisher = "S. Karger AG",
number = "3",

}

TY - JOUR

T1 - Mechanisms of photoinduced cochlear ischemia in the guinea pig

AU - Saito, H.

AU - Ogawa, Kaoru

AU - Inoue, Y.

AU - Kanzaki, J.

AU - Harada, T.

AU - Hoya, N.

PY - 2001/5

Y1 - 2001/5

N2 - An experimental model of cochlear ischemia using the photosensitization reaction of rose bengal (RB) is reported. Ischemia was induced by the systemic administration of RB solution and localized irradiation of the cochlea with a green light of specific wavelength for RB activation. After the RB solution was administered, the cochlear blood flow was temporarily increased and then decreased, and the latency of wave I of the auditory brainstem response increased and the amplitude of wave I decreased after RB injection and finally wave I disappeared completely. A histological study revealed thrombus formation in the vessels of the irradiated cochlea not only in the stria vascularis and the spiral ligament, but also in the modiolus. L-Histidine, a scavenger of singlet oxygen (1O2) and hydroxyl radical (·OH), significantly prolonged cochlear dysfunction, but D-mannitol, a specific scavenger of ·OH did not, suggesting that 1O2 has an important role in this reaction.

AB - An experimental model of cochlear ischemia using the photosensitization reaction of rose bengal (RB) is reported. Ischemia was induced by the systemic administration of RB solution and localized irradiation of the cochlea with a green light of specific wavelength for RB activation. After the RB solution was administered, the cochlear blood flow was temporarily increased and then decreased, and the latency of wave I of the auditory brainstem response increased and the amplitude of wave I decreased after RB injection and finally wave I disappeared completely. A histological study revealed thrombus formation in the vessels of the irradiated cochlea not only in the stria vascularis and the spiral ligament, but also in the modiolus. L-Histidine, a scavenger of singlet oxygen (1O2) and hydroxyl radical (·OH), significantly prolonged cochlear dysfunction, but D-mannitol, a specific scavenger of ·OH did not, suggesting that 1O2 has an important role in this reaction.

KW - Cochlear ischemia

KW - Experimental model

KW - L-Histidine

KW - Photosensitizer

KW - Rose bengal

KW - Singlet oxygen

UR - http://www.scopus.com/inward/record.url?scp=0035006511&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035006511&partnerID=8YFLogxK

U2 - 10.1159/000055731

DO - 10.1159/000055731

M3 - Article

C2 - 11359092

AN - SCOPUS:0035006511

VL - 63

SP - 148

EP - 154

JO - ORL; journal for oto-rhino-laryngology and its related specialties

JF - ORL; journal for oto-rhino-laryngology and its related specialties

SN - 0301-1569

IS - 3

ER -