Megakaryocytes are essential for HSC quiescence through the production of thrombopoietin

Ayako Nakamura-Ishizu, Keiyo Takubo, Masato Fujioka, Toshio Suda

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Tissue homeostasis demands regulatory feedback, suggesting that hematopoietic stem cell (HSC) activity is controlled in part by HSC progeny. Yet, cell extrinsic HSC regulation has been well characterized only in niche cells of non-hematopoietic origin. Here we identify feedback regulation of HSCs by megakaryocytes (Mks), which are mature hematopoietic cells, through production of thrombopoietin (Thpo), a cytokine pertinent for HSC maintenance. Induced ablation of Mk cell population in mice perturbed quiescent HSCs in bone marrow (BM). The ablation of Mks resulted in decreased intra-BM Thpo concentration presumably due to Thpo production by Mks. Thpo administration Mk ablated mice restored HSC functions. Overall, our study establishes Mk as an essential cellular component of the HSC niche and delineates cytokine-oriented regulation of HSCs by their own progeny.

Original languageEnglish
Pages (from-to)353-357
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume454
Issue number2
DOIs
Publication statusPublished - 2014 Nov 14

Keywords

  • Bone marrow
  • Hematopoietic stem cells
  • Megakaryocytes
  • Niche
  • Thrombopoietin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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