Membrane potential measurement of protoplasts isolated from vigna mungo hypocotyl using a fluorescent probe, diS-C3-(5)

Kotaro Oka, Shigehiro Naitou, Motoko Yoshida, Hideo Ishikawa, Eiji Ohta, Makoto Sakata

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Membrane potentials of protoplasts isolated from Vigna mungo hypocotyl segments were measured using the fluorescent probe diS-C3-(5). The fluorescence intensity changed in response to the external K+ concentration. Membrane potential was estimated to be inside negative (-85±8 mV at 0.1 mM KCl) from the Nernst equation for K+. The membrane potential was not affected by DCCD (50 μM) or low temperature (5°C). Addition of 0.5 mM Ca2+ to the protoplast suspension markedly depolarized the membrane potential, and subsequent EDTA treatment repolarized it to the initial level. The Ca2+ effect on the membrane potential may be due to change in the permeability ratio of Cl- to K+.

Original languageEnglish
Pages (from-to)843-849
Number of pages7
JournalPlant and Cell Physiology
Volume28
Issue number5
Publication statusPublished - 1987 Jul

Fingerprint

Hypocotyl
Protoplast
Vigna mungo
Membrane Potential
Protoplasts
Fluorescent Dyes
membrane potential
Membrane Potentials
hypocotyls
protoplasts
Probe
probe
membrane
Membranes
Dicyclohexylcarbodiimide
calcium
Ethylenediaminetetraacetic acid
EDTA
Edetic Acid
Permeability

Keywords

  • Ca2+ effect
  • DiS-C3-(5)
  • Membrane potential
  • TPP+ selective electrode
  • Vigna mungo (protoplats)

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Membrane potential measurement of protoplasts isolated from vigna mungo hypocotyl using a fluorescent probe, diS-C3-(5). / Oka, Kotaro; Naitou, Shigehiro; Yoshida, Motoko; Ishikawa, Hideo; Ohta, Eiji; Sakata, Makoto.

In: Plant and Cell Physiology, Vol. 28, No. 5, 07.1987, p. 843-849.

Research output: Contribution to journalArticle

Oka, Kotaro ; Naitou, Shigehiro ; Yoshida, Motoko ; Ishikawa, Hideo ; Ohta, Eiji ; Sakata, Makoto. / Membrane potential measurement of protoplasts isolated from vigna mungo hypocotyl using a fluorescent probe, diS-C3-(5). In: Plant and Cell Physiology. 1987 ; Vol. 28, No. 5. pp. 843-849.
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