Membrane potential measurement of protoplasts isolated from vigna mungo hypocotyl using a fluorescent probe, diS-C3-(5)

Kotaro Oka, Shigehiro Naitou, Motoko Yoshida, Hideo Ishikawa, Eiji Ohta, Makoto Sakata

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Membrane potentials of protoplasts isolated from Vigna mungo hypocotyl segments were measured using the fluorescent probe diS-C3-(5). The fluorescence intensity changed in response to the external K+ concentration. Membrane potential was estimated to be inside negative (-85±8 mV at 0.1 mM KCl) from the Nernst equation for K+. The membrane potential was not affected by DCCD (50 μM) or low temperature (5°C). Addition of 0.5 mM Ca2+ to the protoplast suspension markedly depolarized the membrane potential, and subsequent EDTA treatment repolarized it to the initial level. The Ca2+ effect on the membrane potential may be due to change in the permeability ratio of Cl- to K+.

Original languageEnglish
Pages (from-to)843-849
Number of pages7
JournalPlant and Cell Physiology
Volume28
Issue number5
DOIs
Publication statusPublished - 1987 Jul

Keywords

  • DiS-C3-(5)
  • Membrane potential
  • Vigna mungo (protoplats)

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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