Membrane Protein Transfer from Human Erythrocyte Ghosts to Liposomes Containing an Artificial Boundary Lipid

Kenichi Suzuki, Yukihisa Okumura, Junzo Sunamoto, Toshinori Sato

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

When human erythrocyte ghost was exposed to a liposome that contains an artificial boundary lipid (1, 2-dimyristoylamido-l, 2-deoxyphosphatidylcholine, coded as D14DPC), various kinds of membrane proteins and several lipids effectively transferred from the ghost to the liposome. The amount of proteins transferred increased with an increase in the D14DPC content of the liposome. The SDS-PAGE analysis revealed the bands at 88 kDa, 74 kDa and 55 kDa in the liposome after the exposure. No erythrocyte proteins larger than 88 kDa were detected. Transfer of AChE and band 3 was detected by using the enzymatic activity of AChE and a fluorescence probe specific to band 3. For the membrane protein transfer, an induction period was usually observed. The duration of the induction period was almost same for both of proteins, AChE and band 3. However, the transfer efficiency of band 3 was much less than that of AChE. Except the membrane proteins, a detectable amount of cholesterol also transferred from the ghost to the liposome. On the other hand, a significant amount of phospholipids simultaneously transferred from the liposome to the ghost.

Original languageEnglish
Pages (from-to)93-97
Number of pages5
JournalProceedings of the Japan Academy Series B: Physical and Biological Sciences
Volume71
Issue number3
DOIs
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Keywords

  • Artificial boundary lipid
  • acetylcho-linesterase (AChE)
  • band 3
  • direct membrane protein transfer
  • human erythrocyte ghost
  • liposome

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Physics and Astronomy(all)

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