Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the cupin superfamily: A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors

Takamasa Uekita, Isamu Gotoh, Takeshi Kinoshita, Yoshifumi Itoh, Hiroshi Sato, Takayuki Shiomi, Yasunori Okada, Motoharu Seiki

Research output: Contribution to journalArticle

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Abstract

Membrane-type 1 matrix metalloproteinase (MT1-MMP/MMP-14) is an enzyme that promotes tumor cell invasion in tissues. Although the proteolytic activity of MT1-MMP is indispensable for invasion, it is also regulated by functions of the cytoplasmic tail. In this study we obtained a new human gene whose product binds to the tail sequence in yeast. The product, MTCBP-1, is a 19-kDa protein that belongs to the newly proposed Cupin superfamily composed of proteins with diverse functions. MTCBP-1 expressed in cells formed a complex with MT1-MMP and co-localized at the membrane. It was also detected in both the cytoplasm and nucleus, where MT1-MMP does not exist. In human tumor cell lines MTCBP-1 expression was significantly low compared with non-transformed fibroblasts, and enforced expression of MTCBP-1 inhibited the activity of MT1-MMP in promoting cell migration and invasion. MTCBP-1 showed significant homology to the bacterial aci-reductone dioxygenase, which is an enzyme in methionine metabolism. The C-terminal part of MTCBP-1 is identical to Sip-L, which is reported to be important for human hepatitis C virus replication. Thus, MTCBP-1 may have multiple functions other than the regulation of MT1-MMP, which presumably depends on the subcellular compartment.

Original languageEnglish
Pages (from-to)12734-12743
Number of pages10
JournalJournal of Biological Chemistry
Volume279
Issue number13
DOIs
Publication statusPublished - 2004 Mar 26

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Matrix Metalloproteinase 14
Tail
Tumors
Carrier Proteins
Neoplasms
Proteins
Cells
Dioxygenases
Enzymes
Fibroblasts
Virus Replication
Tumor Cell Line
Matrix Metalloproteinases
Viruses
Metabolism
Hepacivirus
Methionine
Yeast
Cell Movement
Cytoplasm

ASJC Scopus subject areas

  • Biochemistry

Cite this

Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the cupin superfamily : A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. / Uekita, Takamasa; Gotoh, Isamu; Kinoshita, Takeshi; Itoh, Yoshifumi; Sato, Hiroshi; Shiomi, Takayuki; Okada, Yasunori; Seiki, Motoharu.

In: Journal of Biological Chemistry, Vol. 279, No. 13, 26.03.2004, p. 12734-12743.

Research output: Contribution to journalArticle

Uekita, Takamasa ; Gotoh, Isamu ; Kinoshita, Takeshi ; Itoh, Yoshifumi ; Sato, Hiroshi ; Shiomi, Takayuki ; Okada, Yasunori ; Seiki, Motoharu. / Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the cupin superfamily : A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 13. pp. 12734-12743.
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