Memory of cell shape biases stochastic fate decision-making despite mitotic rounding

Takashi Akanuma, Cong Chen, Tetsuo Sato, Roeland M H Merks, Thomas N. Sato

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cell shape influences function, and the current model suggests that such shape effect is transient. However, cells dynamically change their shapes, thus, the critical question is whether shape information remains influential on future cell function even after the original shape is lost. We address this question by integrating experimental and computational approaches. Quantitative live imaging of asymmetric cell-fate decision-making and their live shape manipulation demonstrates that cellular eccentricity of progenitor cell indeed biases stochastic fate decisions of daughter cells despite mitotic rounding. Modelling and simulation indicates that polarized localization of Delta protein instructs by the progenitor eccentricity is an origin of the bias. Simulation with varying parameters predicts that diffusion rate and abundance of Delta molecules quantitatively influence the bias. These predictions are experimentally validated by physical and genetic methods, showing that cells exploit a mechanism reported herein to influence their future fates based on their past shape despite dynamic shape changes.

Original languageEnglish
Article number11963
JournalNature Communications
Volume7
DOIs
Publication statusPublished - 2016 Jun 28
Externally publishedYes

Fingerprint

Cell Shape
decision making
Decision Making
Decision making
Data storage equipment
cells
Imaging techniques
Molecules
eccentricity
Stem Cells
manipulators
simulation
proteins
delta protein
predictions

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Memory of cell shape biases stochastic fate decision-making despite mitotic rounding. / Akanuma, Takashi; Chen, Cong; Sato, Tetsuo; Merks, Roeland M H; Sato, Thomas N.

In: Nature Communications, Vol. 7, 11963, 28.06.2016.

Research output: Contribution to journalArticle

Akanuma, Takashi ; Chen, Cong ; Sato, Tetsuo ; Merks, Roeland M H ; Sato, Thomas N. / Memory of cell shape biases stochastic fate decision-making despite mitotic rounding. In: Nature Communications. 2016 ; Vol. 7.
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