TY - JOUR
T1 - Menstrual cycle-associated regulation of anabolic and catabolic enzymes causes luteal phase-characteristic expression of sulfatide in human endometrium
AU - Kamei, Kazuhiko
AU - Kubushiro, Kaneyuki
AU - Fuju, Takuma
AU - Tsukazaki, Katsumi
AU - Nozawa, Shiro
AU - Iwamori, Masao
N1 - Funding Information:
Supported in part by grants for General Scientific Research (No. 631540224688 and No. 40224688) from the Ministry of Education, Science, and Culture, Japan, and by grants from the Keio University Medical Research Foundation.
PY - 1997
Y1 - 1997
N2 - OBJECTIVE: Our purpose was to investigate the metabolic background of the expression of sulfoglycolipids in human endometrium during the luteal phase. STUDY DESIGN: We investigated the expression of sulfoglycolipids by thin-layer chromatography immunostaining and the activities of galactosylceramide sulfotransferase and arylsulfatase A, which regulate the synthesis and degradation of sulfoglycolipid. In addition, arylsulfatase A messenger ribonucleic acid was studied by reverse transcriptase polymerase chain reaction. RESULTS: Sulfoglycolipid expression showed a marked increase in the luteal phase but not in the follicular phase, whereas sialoglycolipids remained relatively constant. The increase of sulfoglycolipids was found to be due to 4.5-fold increased activation of sulfotransferase and a concurrent reduction of arylsulfatase A activity in the luteal phase. Arylsulfatase A messenger ribonucleic acid was detected in both phases and showed no significant changes. CONCLUSIONS: These findings suggest that increased sulfoglycolipid expression in the luteal phase is due to the simultaneous regulation of sulfotransferase and arylsulfatase A, probably by sex steroid hormones.
AB - OBJECTIVE: Our purpose was to investigate the metabolic background of the expression of sulfoglycolipids in human endometrium during the luteal phase. STUDY DESIGN: We investigated the expression of sulfoglycolipids by thin-layer chromatography immunostaining and the activities of galactosylceramide sulfotransferase and arylsulfatase A, which regulate the synthesis and degradation of sulfoglycolipid. In addition, arylsulfatase A messenger ribonucleic acid was studied by reverse transcriptase polymerase chain reaction. RESULTS: Sulfoglycolipid expression showed a marked increase in the luteal phase but not in the follicular phase, whereas sialoglycolipids remained relatively constant. The increase of sulfoglycolipids was found to be due to 4.5-fold increased activation of sulfotransferase and a concurrent reduction of arylsulfatase A activity in the luteal phase. Arylsulfatase A messenger ribonucleic acid was detected in both phases and showed no significant changes. CONCLUSIONS: These findings suggest that increased sulfoglycolipid expression in the luteal phase is due to the simultaneous regulation of sulfotransferase and arylsulfatase A, probably by sex steroid hormones.
KW - Arylsulfatase a
KW - Galactosylceramide sulfotransferase
KW - Sulfoglycolipids
KW - Thin-layer chromatography immunostaining
UR - http://www.scopus.com/inward/record.url?scp=0030635695&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030635695&partnerID=8YFLogxK
U2 - 10.1016/S0002-9378(97)80027-0
DO - 10.1016/S0002-9378(97)80027-0
M3 - Article
C2 - 9024105
AN - SCOPUS:0030635695
VL - 176
SP - 142
EP - 149
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
SN - 0002-9378
IS - 1 I
ER -