Mesenchylmal stem cell culture on poly(N-isopropylacrylamide) hydrogel with repeated thermo-stimulation

Aya Mizutani Akimoto, Erika Hasuike Niitsu, Kenichi Nagase, Teruo Okano, Hideko Kanazawa, Ryo Yoshida

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We prepared thermoresponsive hydrogels by mixing poly(N-isopropylacrylamide) (PNIPAAm) derivatives as the main chain components, octa arm polyethylene glycol (PEG) as a crosslinker, and the Arg-Gly-Asp-Ser (RGDS) peptides as cell adhesion units. Human bone marrow derived mesenchymal stem cells (hbmMSCs) were cultured on the hydrogels. The PNIPAAm gel prepared by the post crosslinking gelation method was revealed to be cytocompatible and showed temperature dependent changes in mechanical properties. Repeated changes in the swelling ratio of the PNIPAAm gel affected the shape of the hbmMSCs. With respect to both cytocompatibility and reversibility of changes in mechanical properties, the PNIPAAm gel system could be potentially useful for the analysis of cell mechanobiology.

Original languageEnglish
Article number1253
JournalInternational Journal of Molecular Sciences
Volume19
Issue number4
DOIs
Publication statusPublished - 2018 Apr 21

Fingerprint

stem cells
Hydrogel
Stem cells
stimulation
Cell culture
Hydrogels
arginyl-glycyl-aspartyl-serine
Stem Cells
Gels
Cell Culture Techniques
bone marrow
gels
Bone
Mesenchymal Stromal Cells
mechanical properties
Mechanical properties
Bone Marrow
Cell adhesion
gelation
Gelation

Keywords

  • Cell culture
  • Hydrogel
  • mechanobiology
  • Mesenchymal stem cell
  • poly(N-isopropylacrylamide)

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Mesenchylmal stem cell culture on poly(N-isopropylacrylamide) hydrogel with repeated thermo-stimulation. / Akimoto, Aya Mizutani; Niitsu, Erika Hasuike; Nagase, Kenichi; Okano, Teruo; Kanazawa, Hideko; Yoshida, Ryo.

In: International Journal of Molecular Sciences, Vol. 19, No. 4, 1253, 21.04.2018.

Research output: Contribution to journalArticle

@article{62243c84bb9c49a9a630d9a9868f3652,
title = "Mesenchylmal stem cell culture on poly(N-isopropylacrylamide) hydrogel with repeated thermo-stimulation",
abstract = "We prepared thermoresponsive hydrogels by mixing poly(N-isopropylacrylamide) (PNIPAAm) derivatives as the main chain components, octa arm polyethylene glycol (PEG) as a crosslinker, and the Arg-Gly-Asp-Ser (RGDS) peptides as cell adhesion units. Human bone marrow derived mesenchymal stem cells (hbmMSCs) were cultured on the hydrogels. The PNIPAAm gel prepared by the post crosslinking gelation method was revealed to be cytocompatible and showed temperature dependent changes in mechanical properties. Repeated changes in the swelling ratio of the PNIPAAm gel affected the shape of the hbmMSCs. With respect to both cytocompatibility and reversibility of changes in mechanical properties, the PNIPAAm gel system could be potentially useful for the analysis of cell mechanobiology.",
keywords = "Cell culture, Hydrogel, mechanobiology, Mesenchymal stem cell, poly(N-isopropylacrylamide)",
author = "Akimoto, {Aya Mizutani} and Niitsu, {Erika Hasuike} and Kenichi Nagase and Teruo Okano and Hideko Kanazawa and Ryo Yoshida",
year = "2018",
month = "4",
day = "21",
doi = "10.3390/ijms19041253",
language = "English",
volume = "19",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "4",

}

TY - JOUR

T1 - Mesenchylmal stem cell culture on poly(N-isopropylacrylamide) hydrogel with repeated thermo-stimulation

AU - Akimoto, Aya Mizutani

AU - Niitsu, Erika Hasuike

AU - Nagase, Kenichi

AU - Okano, Teruo

AU - Kanazawa, Hideko

AU - Yoshida, Ryo

PY - 2018/4/21

Y1 - 2018/4/21

N2 - We prepared thermoresponsive hydrogels by mixing poly(N-isopropylacrylamide) (PNIPAAm) derivatives as the main chain components, octa arm polyethylene glycol (PEG) as a crosslinker, and the Arg-Gly-Asp-Ser (RGDS) peptides as cell adhesion units. Human bone marrow derived mesenchymal stem cells (hbmMSCs) were cultured on the hydrogels. The PNIPAAm gel prepared by the post crosslinking gelation method was revealed to be cytocompatible and showed temperature dependent changes in mechanical properties. Repeated changes in the swelling ratio of the PNIPAAm gel affected the shape of the hbmMSCs. With respect to both cytocompatibility and reversibility of changes in mechanical properties, the PNIPAAm gel system could be potentially useful for the analysis of cell mechanobiology.

AB - We prepared thermoresponsive hydrogels by mixing poly(N-isopropylacrylamide) (PNIPAAm) derivatives as the main chain components, octa arm polyethylene glycol (PEG) as a crosslinker, and the Arg-Gly-Asp-Ser (RGDS) peptides as cell adhesion units. Human bone marrow derived mesenchymal stem cells (hbmMSCs) were cultured on the hydrogels. The PNIPAAm gel prepared by the post crosslinking gelation method was revealed to be cytocompatible and showed temperature dependent changes in mechanical properties. Repeated changes in the swelling ratio of the PNIPAAm gel affected the shape of the hbmMSCs. With respect to both cytocompatibility and reversibility of changes in mechanical properties, the PNIPAAm gel system could be potentially useful for the analysis of cell mechanobiology.

KW - Cell culture

KW - Hydrogel

KW - mechanobiology

KW - Mesenchymal stem cell

KW - poly(N-isopropylacrylamide)

UR - http://www.scopus.com/inward/record.url?scp=85045943994&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045943994&partnerID=8YFLogxK

U2 - 10.3390/ijms19041253

DO - 10.3390/ijms19041253

M3 - Article

VL - 19

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 4

M1 - 1253

ER -