Abstract
Bone marrow (BM) cells are reported to contribute to the process of regeneration following myocardial infarction. The present study examined two independent clonal studies to determine the origin of bone marrow (BM)-derived cardiomyocytes. First, we transplanted single CD34 -c-kit +Sca-1 +lineage - side population cells (hematopoietic stem cells) from enhanced green fluorescent protein (EGFP)-transgenic mice into lethally irradiated mice, induced myocardial infarction, and treated them with G-CSF to mobilize stem cells. At 8 weeks, we could not find any EGFP + cardiomyocytes. In contrast, more than 5000 EGFP + cardiomyocytes were observed in whole BM cell-transplanted mice, suggesting that they were derived from non-hematopoietic cells. Next, clonally purified mesenchymal stem cells (MSC) that expressed EGFP in the cardiomyocyte-specific manner were transplanted directly into BM of lethally irradiated mice, and similar experiment was performed. EGFP + actinin + cells were observed in the ischemic myocardium, indicating that MSC had been mobilized and differentiated into cardiomyocytes. Together, these results suggest that the origin of the BM-derived cardiomyocytes is MSC.
| Original language | English |
|---|---|
| Pages (from-to) | 1940-1943 |
| Number of pages | 4 |
| Journal | Kidney International |
| Volume | 68 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2005 Nov |
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ASJC Scopus subject areas
- Nephrology
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Mesenchymal, but not hematopoietic, stem cells can be mobilized and differentiate into cardiomyocytes after myocardial infarction in mice. / Fukuda, Keiichi; Fujita, Jun.
In: Kidney International, Vol. 68, No. 5, 11.2005, p. 1940-1943.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Mesenchymal, but not hematopoietic, stem cells can be mobilized and differentiate into cardiomyocytes after myocardial infarction in mice
AU - Fukuda, Keiichi
AU - Fujita, Jun
PY - 2005/11
Y1 - 2005/11
N2 - Bone marrow (BM) cells are reported to contribute to the process of regeneration following myocardial infarction. The present study examined two independent clonal studies to determine the origin of bone marrow (BM)-derived cardiomyocytes. First, we transplanted single CD34 -c-kit +Sca-1 +lineage - side population cells (hematopoietic stem cells) from enhanced green fluorescent protein (EGFP)-transgenic mice into lethally irradiated mice, induced myocardial infarction, and treated them with G-CSF to mobilize stem cells. At 8 weeks, we could not find any EGFP + cardiomyocytes. In contrast, more than 5000 EGFP + cardiomyocytes were observed in whole BM cell-transplanted mice, suggesting that they were derived from non-hematopoietic cells. Next, clonally purified mesenchymal stem cells (MSC) that expressed EGFP in the cardiomyocyte-specific manner were transplanted directly into BM of lethally irradiated mice, and similar experiment was performed. EGFP + actinin + cells were observed in the ischemic myocardium, indicating that MSC had been mobilized and differentiated into cardiomyocytes. Together, these results suggest that the origin of the BM-derived cardiomyocytes is MSC.
AB - Bone marrow (BM) cells are reported to contribute to the process of regeneration following myocardial infarction. The present study examined two independent clonal studies to determine the origin of bone marrow (BM)-derived cardiomyocytes. First, we transplanted single CD34 -c-kit +Sca-1 +lineage - side population cells (hematopoietic stem cells) from enhanced green fluorescent protein (EGFP)-transgenic mice into lethally irradiated mice, induced myocardial infarction, and treated them with G-CSF to mobilize stem cells. At 8 weeks, we could not find any EGFP + cardiomyocytes. In contrast, more than 5000 EGFP + cardiomyocytes were observed in whole BM cell-transplanted mice, suggesting that they were derived from non-hematopoietic cells. Next, clonally purified mesenchymal stem cells (MSC) that expressed EGFP in the cardiomyocyte-specific manner were transplanted directly into BM of lethally irradiated mice, and similar experiment was performed. EGFP + actinin + cells were observed in the ischemic myocardium, indicating that MSC had been mobilized and differentiated into cardiomyocytes. Together, these results suggest that the origin of the BM-derived cardiomyocytes is MSC.
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U2 - 10.1111/j.1523-1755.2005.00624.x
DO - 10.1111/j.1523-1755.2005.00624.x
M3 - Article
C2 - 16221170
AN - SCOPUS:30944448824
VL - 68
SP - 1940
EP - 1943
JO - Kidney International
JF - Kidney International
SN - 0085-2538
IS - 5
ER -