Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation

Fumio Arai, Osamu Ohneda, Takeshi Miyamoto, Xiu Qin Zhang, Toshio Suda

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

Perichondrium in fetal limb is composed of undifferentiated mesenchymal cells. However, the multipotency of cells in this region and the role of perichondrium in bone marrow formation are not well understood. In this report, we purified and characterized perichondrial cells using a monoclonal antibody against activated leukocyte cell adhesion molecule (ALCAM) and investigated the role of perichondrial cells in hematopoietic bone marrow formation. ALCAM is expressed on hematopoietic cells, endothelial cells, bone marrow stromal cells, and mesenchymal stem cells and mediates homophilic (ALCAM-ALCAM)/heterophilic (ALCAM-CD6) cell adhesion. Here we show by immunohistochemical staining that ALCAM is expressed in perichondrium. ALCAM+ perichondrial cells isolated by FACS® exhibit the characteristics of mesenchymal stem cells. ALCAM+ cells can differentiate into osteoblasts, adipocytes, chondrocytes, and stromal cells, which can support osteoclastogenesis, hematopoiesis, and angiogenesis. Furthermore, the addition of ALCAM-Fc or CD6-Fc to the metatarsal culture, the invasion of the blood vessels to a cartilage was inhibited. Our findings indicate that ALCAM+ perichondrial cells participate in vascular invasion by recruiting osteoclasts and vessels. These findings suggest that perichondrium might serve as a stem cell reservoir and play an important role in the early development of a bone and bone marrow.

Original languageEnglish
Pages (from-to)1549-1563
Number of pages15
JournalJournal of Experimental Medicine
Volume195
Issue number12
DOIs
Publication statusPublished - 2002 Jun 17

Fingerprint

Activated-Leukocyte Cell Adhesion Molecule
Mesenchymal Stromal Cells
Osteogenesis
Bone Marrow
Blood Vessels
Metatarsal Bones
Hematopoiesis
Osteoclasts
Stromal Cells
Chondrocytes
Osteoblasts
Adipocytes
Cell Adhesion
Cartilage

Keywords

  • Angiogenesis
  • Differentiation
  • Homophilic cell adhesion
  • Limb bud
  • Stem cell

ASJC Scopus subject areas

  • Immunology

Cite this

Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation. / Arai, Fumio; Ohneda, Osamu; Miyamoto, Takeshi; Zhang, Xiu Qin; Suda, Toshio.

In: Journal of Experimental Medicine, Vol. 195, No. 12, 17.06.2002, p. 1549-1563.

Research output: Contribution to journalArticle

Arai, Fumio ; Ohneda, Osamu ; Miyamoto, Takeshi ; Zhang, Xiu Qin ; Suda, Toshio. / Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation. In: Journal of Experimental Medicine. 2002 ; Vol. 195, No. 12. pp. 1549-1563.
@article{a706466d8f7f4fc690b9298f2d411ee4,
title = "Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation",
abstract = "Perichondrium in fetal limb is composed of undifferentiated mesenchymal cells. However, the multipotency of cells in this region and the role of perichondrium in bone marrow formation are not well understood. In this report, we purified and characterized perichondrial cells using a monoclonal antibody against activated leukocyte cell adhesion molecule (ALCAM) and investigated the role of perichondrial cells in hematopoietic bone marrow formation. ALCAM is expressed on hematopoietic cells, endothelial cells, bone marrow stromal cells, and mesenchymal stem cells and mediates homophilic (ALCAM-ALCAM)/heterophilic (ALCAM-CD6) cell adhesion. Here we show by immunohistochemical staining that ALCAM is expressed in perichondrium. ALCAM+ perichondrial cells isolated by FACS{\circledR} exhibit the characteristics of mesenchymal stem cells. ALCAM+ cells can differentiate into osteoblasts, adipocytes, chondrocytes, and stromal cells, which can support osteoclastogenesis, hematopoiesis, and angiogenesis. Furthermore, the addition of ALCAM-Fc or CD6-Fc to the metatarsal culture, the invasion of the blood vessels to a cartilage was inhibited. Our findings indicate that ALCAM+ perichondrial cells participate in vascular invasion by recruiting osteoclasts and vessels. These findings suggest that perichondrium might serve as a stem cell reservoir and play an important role in the early development of a bone and bone marrow.",
keywords = "Angiogenesis, Differentiation, Homophilic cell adhesion, Limb bud, Stem cell",
author = "Fumio Arai and Osamu Ohneda and Takeshi Miyamoto and Zhang, {Xiu Qin} and Toshio Suda",
year = "2002",
month = "6",
day = "17",
doi = "10.1084/jem.20011700",
language = "English",
volume = "195",
pages = "1549--1563",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "12",

}

TY - JOUR

T1 - Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation

AU - Arai, Fumio

AU - Ohneda, Osamu

AU - Miyamoto, Takeshi

AU - Zhang, Xiu Qin

AU - Suda, Toshio

PY - 2002/6/17

Y1 - 2002/6/17

N2 - Perichondrium in fetal limb is composed of undifferentiated mesenchymal cells. However, the multipotency of cells in this region and the role of perichondrium in bone marrow formation are not well understood. In this report, we purified and characterized perichondrial cells using a monoclonal antibody against activated leukocyte cell adhesion molecule (ALCAM) and investigated the role of perichondrial cells in hematopoietic bone marrow formation. ALCAM is expressed on hematopoietic cells, endothelial cells, bone marrow stromal cells, and mesenchymal stem cells and mediates homophilic (ALCAM-ALCAM)/heterophilic (ALCAM-CD6) cell adhesion. Here we show by immunohistochemical staining that ALCAM is expressed in perichondrium. ALCAM+ perichondrial cells isolated by FACS® exhibit the characteristics of mesenchymal stem cells. ALCAM+ cells can differentiate into osteoblasts, adipocytes, chondrocytes, and stromal cells, which can support osteoclastogenesis, hematopoiesis, and angiogenesis. Furthermore, the addition of ALCAM-Fc or CD6-Fc to the metatarsal culture, the invasion of the blood vessels to a cartilage was inhibited. Our findings indicate that ALCAM+ perichondrial cells participate in vascular invasion by recruiting osteoclasts and vessels. These findings suggest that perichondrium might serve as a stem cell reservoir and play an important role in the early development of a bone and bone marrow.

AB - Perichondrium in fetal limb is composed of undifferentiated mesenchymal cells. However, the multipotency of cells in this region and the role of perichondrium in bone marrow formation are not well understood. In this report, we purified and characterized perichondrial cells using a monoclonal antibody against activated leukocyte cell adhesion molecule (ALCAM) and investigated the role of perichondrial cells in hematopoietic bone marrow formation. ALCAM is expressed on hematopoietic cells, endothelial cells, bone marrow stromal cells, and mesenchymal stem cells and mediates homophilic (ALCAM-ALCAM)/heterophilic (ALCAM-CD6) cell adhesion. Here we show by immunohistochemical staining that ALCAM is expressed in perichondrium. ALCAM+ perichondrial cells isolated by FACS® exhibit the characteristics of mesenchymal stem cells. ALCAM+ cells can differentiate into osteoblasts, adipocytes, chondrocytes, and stromal cells, which can support osteoclastogenesis, hematopoiesis, and angiogenesis. Furthermore, the addition of ALCAM-Fc or CD6-Fc to the metatarsal culture, the invasion of the blood vessels to a cartilage was inhibited. Our findings indicate that ALCAM+ perichondrial cells participate in vascular invasion by recruiting osteoclasts and vessels. These findings suggest that perichondrium might serve as a stem cell reservoir and play an important role in the early development of a bone and bone marrow.

KW - Angiogenesis

KW - Differentiation

KW - Homophilic cell adhesion

KW - Limb bud

KW - Stem cell

UR - http://www.scopus.com/inward/record.url?scp=0037124308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037124308&partnerID=8YFLogxK

U2 - 10.1084/jem.20011700

DO - 10.1084/jem.20011700

M3 - Article

C2 - 12070283

AN - SCOPUS:0037124308

VL - 195

SP - 1549

EP - 1563

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 12

ER -