Met72Thr polymorphism of pigment epithelium-derived factor gene and susceptibility to age-related macular degeneration

S. Yamagishi, K. Nakamura, Hiroyoshi Inoue, M. Takeuchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Age-related macular degeneration (ARMD) is the most common cause of acquired blindness among the people of occupational age. Although the pathogenesis of ARMD is not fully understood, several studies suggest a possible contribution of a genetic factor in the development and progression of ARMD. Pigment epithelium-derived factor (PEDF), a glycoprotein that belongs to the superfamily of serine protease inhibitors, was first purified from the conditioned media of human retinal pigment epithelial cells as a factor with potent neuronal differentiating activity in human retinoblastoma cells. Recently, PEDF has been shown to be a highly effective inhibitor of angiogenesis in cell culture and animal models. In addition, PEDF has been found in the vitreous, and its levels were decreased in angiogenic eye diseases, thus suggesting that a loss of PEDF in the eye is functionally important in the pathogenesis of ARMD. A functional amino acid change, a methionine to threonine polymorphism (Met72Thr polymorphism) at codon 72 in exon 3 (T/C polymorphism) of the PEDF gene, that results in the formation of BsstSI restriction site, has recently been identified. Since it is well known that a single nucleotide polymorphism and resultant amino acid change often alters the activity or expression level of the target protein, we would like to propose here a novel hypothesis that the Met72Thr polymorphism (T/C polymorphism) of PEDF gene may be a genetic marker for ARMD. Are genotype and allele frequencies of the Met72Thr polymorphism (T/C polymorphism) different between the patients with or without ARMD? Is this polymorphism associated with disease severity and progression? If the answer is yes, does this Met72Thr polymorphism regulate the vitreous levels of PEDF? These clinical studies could provide us with information whether this genetic variant of the PEDF gene could present an attractive candidate susceptibility gene for ARMD.

Original languageEnglish
Pages (from-to)1202-1204
Number of pages3
JournalMedical Hypotheses
Volume64
Issue number6
DOIs
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Macular Degeneration
Genes
Amino Acids
Serine Proteinase Inhibitors
Angiogenesis Inhibitors
Retinal Pigments
Retinoblastoma
Eye Diseases
pigment epithelium-derived factor
Threonine
Blindness
Conditioned Culture Medium
Genetic Markers
Gene Frequency
Human Activities
Codon
Methionine
Single Nucleotide Polymorphism
Disease Progression
Exons

ASJC Scopus subject areas

  • Developmental Biology
  • Medicine(all)
  • Drug Discovery

Cite this

Met72Thr polymorphism of pigment epithelium-derived factor gene and susceptibility to age-related macular degeneration. / Yamagishi, S.; Nakamura, K.; Inoue, Hiroyoshi; Takeuchi, M.

In: Medical Hypotheses, Vol. 64, No. 6, 2005, p. 1202-1204.

Research output: Contribution to journalArticle

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