@article{328de6c4a0c141068aed1f6cdcf946dc,
title = "Metabolic-Pathway-Oriented Screening Targeting S-Adenosyl- l -methionine Reveals the Epigenetic Remodeling Activities of Naturally Occurring Catechols",
abstract = "Methyl transfer reactions play important roles in many biological phenomena, wherein the methylation cofactor S-adenosyl-l-methionine (SAM) serves as the important currency to orchestrate those reactions. We have developed a fluorescent-probe-based high-throughput screening (HTS) system to search for the compounds that control cellular SAM levels. HTS with a drug repositioning library revealed the importance of catechol-O-methyltransferase (COMT) and its substrates in controlling the SAM concentrations and histone methylation levels in colorectal tumor cells.",
author = "Shusuke Ogihara and Toru Komatsu and Yukihiro Itoh and Yuka Miyake and Takayoshi Suzuki and Kouichi Yanagi and Yusuke Kimura and Tasuku Ueno and Kenjiro Hanaoka and Hirotatsu Kojima and Takayoshi Okabe and Tetsuo Nagano and Yasuteru Urano",
note = "Funding Information: Financial support for this study was provided by MEXT (24655147, 15H05371, 15K14937, 17K19477, 18H04538, and 19H02846 to T.K.), JST (to T.K.), ImPACT (to Y.U.), and AMED (to Y.U.). The project was also supported by JSPS Core-to-Core program. T.K. was supported by The Naito Foundation, The Mochida Memorial Foundation for Medical and Pharmaceutical Research, and The Tokyo Biochemical Research Foundation. The inhibitor screening project was supported by the Platform Project for Supporting in Drug Discovery and Life Science Research (Platform for Drug Discovery, Informatics, and Structural Life Science) from AMED. Publisher Copyright: Copyright {\textcopyright} 2019 American Chemical Society.",
year = "2020",
month = jan,
day = "8",
doi = "10.1021/jacs.9b08698",
language = "English",
volume = "142",
pages = "21--26",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "1",
}