Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation

Ghulam Jeelani, Dan Sato, Afzal Husain, Aleyla Escueta-de Cadiz, Masahiro Sugimoto, Tomoyoshi Soga, Makoto Suematsu, Tomoyoshi Nozaki

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Encystation, which is cellular differentiation from the motile, proliferative, labile trophozoite form to the dormant, resistant cyst form, is a crucial process found in parasitic and free-living protozoa such as Entamoeba, Giardia, Acanthamoeba, and Balamuthia. Since encystation is an essential process to deal with the adverse external environmental changes during the life cycle, and often integral to the transmission of the diseases, biochemical understanding of the process potentially provides useful measures against the infections caused by this group of protozoa. In this study, we investigated metabolic and transcriptomic changes that occur during encystation in Entamoeba invadens, the reptilian sibling of mammal-infecting E. histolytica, using capillary electrophoresis-tandem mass spectrometry-based metabolite profiling and DNA microarray-based expression profiling. As the encystation progressed, the levels of majority of metabolites involved in glycolysis and nucleotides drastically decreased, indicating energy generation is ceased. Furthermore, the flux of glycolysis was redirected toward chitin wall biosynthesis. We found remarkable temporal increases in biogenic amines such as isoamylamine, isobutylamine, and cadaverine, during the early period of encystation, when the trophozoites form large multicellular aggregates (precyst). We also found remarkable induction of γ-aminobutyric acid (GABA) during encystation. This study has unveiled for the first time the dynamics of the transcriptional and metabolic regulatory networks during encystation, and should help in better understanding of the process in pathogenic eukaryotes, and further development of measures controlling infections they cause.

Original languageEnglish
Article numbere37740
JournalPLoS One
Volume7
Issue number5
DOIs
Publication statusPublished - 2012 May 25

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Protozoa
Entamoeba
encystment
Trophozoites
metabolomics
Glycolysis
Metabolites
Parasites
Biochemical Phenomena
Chemical activation
Cadaverine
Acanthamoeba
Aminobutyrates
Giardia
parasites
Capillary electrophoresis
Biogenic Amines
Mammals
Chitin
Biosynthesis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation. / Jeelani, Ghulam; Sato, Dan; Husain, Afzal; Escueta-de Cadiz, Aleyla; Sugimoto, Masahiro; Soga, Tomoyoshi; Suematsu, Makoto; Nozaki, Tomoyoshi.

In: PLoS One, Vol. 7, No. 5, e37740, 25.05.2012.

Research output: Contribution to journalArticle

Jeelani, G, Sato, D, Husain, A, Escueta-de Cadiz, A, Sugimoto, M, Soga, T, Suematsu, M & Nozaki, T 2012, 'Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation', PLoS One, vol. 7, no. 5, e37740. https://doi.org/10.1371/journal.pone.0037740
Jeelani, Ghulam ; Sato, Dan ; Husain, Afzal ; Escueta-de Cadiz, Aleyla ; Sugimoto, Masahiro ; Soga, Tomoyoshi ; Suematsu, Makoto ; Nozaki, Tomoyoshi. / Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation. In: PLoS One. 2012 ; Vol. 7, No. 5.
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