Metabolic properties of astrocytes differentiated from rat neurospheres

Takato Abe, Shinichi Takahashi, Norihiro Suzuki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The metabolic properties of astroglia differentiated from neurospheres have not been fully assessed. In this study, the glycolytic and oxidative metabolism of glucose in astroglia differentiated from rat tertiary neurospheres (astrogliaNS) was compared with that in astroglia prepared from the striata of embryonic day 16 rats (astrogliaST). In addition to the basal condition, we also investigated energy metabolism under Na+,K+-ATPase activation. Furthermore, the effects of glucose concentration in the culture medium were assessed. No significant differences in 2-deoxy-d-[1-14C]glucose phosphorylation were observed between astrogliaNS and astrogliaST. The rates of l-[U-14C]lactate ([14C]lactate) and d-[U-14C]glucose ([14C]glucose) oxidation were 5.74 ± 0.82 and 2.83 ± 0.4 pmol/60 min/μg protein, respectively, in astrogliaNS grown in low glucose (2 mM) and 3.01 ± 1.03 and 1.77 ± 0.23 pmol/60 min/μg protein, respectively, in astrogliaNS grown in high glucose (22 mM). Neither the [14C]lactate nor the [14C]glucose oxidation rates in astrogliaNS were significantly different from those in astrogliaST. d-aspartate (500 μM) significantly increased the [14C]lactate and [14C]glucose oxidation rates by 127% and 62%, respectively, in astrogliaNS grown in low glucose and by 217% and 115%, respectively, in astrogliaNS grown in high glucose. d-aspartate also increased the oxidation of [14C]lactate and [14C]glucose to 236% and 147% of the control values, respectively, in astrogliaST grown in low glucose and to 174% and 144%, respectively, in astrogliaST grown in high glucose. Rat astroglia differentiated from neurospheres might possess an equivalent capacity for utilizing energy substrates under both basal and activated conditions to that of astroglia prepared from striatum.

Original languageEnglish
Pages (from-to)5-11
Number of pages7
JournalBrain Research
Volume1101
Issue number1
DOIs
Publication statusPublished - 2006 Jul 26

Fingerprint

Astrocytes
Glucose
Lactic Acid
Aspartic Acid
Energy Metabolism
Culture Media
Proteins
Phosphorylation

Keywords

  • Glucose
  • Lactate
  • Neural stem cell

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Metabolic properties of astrocytes differentiated from rat neurospheres. / Abe, Takato; Takahashi, Shinichi; Suzuki, Norihiro.

In: Brain Research, Vol. 1101, No. 1, 26.07.2006, p. 5-11.

Research output: Contribution to journalArticle

Abe, Takato ; Takahashi, Shinichi ; Suzuki, Norihiro. / Metabolic properties of astrocytes differentiated from rat neurospheres. In: Brain Research. 2006 ; Vol. 1101, No. 1. pp. 5-11.
@article{980f390325c74c69994f3e420d89761a,
title = "Metabolic properties of astrocytes differentiated from rat neurospheres",
abstract = "The metabolic properties of astroglia differentiated from neurospheres have not been fully assessed. In this study, the glycolytic and oxidative metabolism of glucose in astroglia differentiated from rat tertiary neurospheres (astrogliaNS) was compared with that in astroglia prepared from the striata of embryonic day 16 rats (astrogliaST). In addition to the basal condition, we also investigated energy metabolism under Na+,K+-ATPase activation. Furthermore, the effects of glucose concentration in the culture medium were assessed. No significant differences in 2-deoxy-d-[1-14C]glucose phosphorylation were observed between astrogliaNS and astrogliaST. The rates of l-[U-14C]lactate ([14C]lactate) and d-[U-14C]glucose ([14C]glucose) oxidation were 5.74 ± 0.82 and 2.83 ± 0.4 pmol/60 min/μg protein, respectively, in astrogliaNS grown in low glucose (2 mM) and 3.01 ± 1.03 and 1.77 ± 0.23 pmol/60 min/μg protein, respectively, in astrogliaNS grown in high glucose (22 mM). Neither the [14C]lactate nor the [14C]glucose oxidation rates in astrogliaNS were significantly different from those in astrogliaST. d-aspartate (500 μM) significantly increased the [14C]lactate and [14C]glucose oxidation rates by 127{\%} and 62{\%}, respectively, in astrogliaNS grown in low glucose and by 217{\%} and 115{\%}, respectively, in astrogliaNS grown in high glucose. d-aspartate also increased the oxidation of [14C]lactate and [14C]glucose to 236{\%} and 147{\%} of the control values, respectively, in astrogliaST grown in low glucose and to 174{\%} and 144{\%}, respectively, in astrogliaST grown in high glucose. Rat astroglia differentiated from neurospheres might possess an equivalent capacity for utilizing energy substrates under both basal and activated conditions to that of astroglia prepared from striatum.",
keywords = "Glucose, Lactate, Neural stem cell",
author = "Takato Abe and Shinichi Takahashi and Norihiro Suzuki",
year = "2006",
month = "7",
day = "26",
doi = "10.1016/j.brainres.2006.05.009",
language = "English",
volume = "1101",
pages = "5--11",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Metabolic properties of astrocytes differentiated from rat neurospheres

AU - Abe, Takato

AU - Takahashi, Shinichi

AU - Suzuki, Norihiro

PY - 2006/7/26

Y1 - 2006/7/26

N2 - The metabolic properties of astroglia differentiated from neurospheres have not been fully assessed. In this study, the glycolytic and oxidative metabolism of glucose in astroglia differentiated from rat tertiary neurospheres (astrogliaNS) was compared with that in astroglia prepared from the striata of embryonic day 16 rats (astrogliaST). In addition to the basal condition, we also investigated energy metabolism under Na+,K+-ATPase activation. Furthermore, the effects of glucose concentration in the culture medium were assessed. No significant differences in 2-deoxy-d-[1-14C]glucose phosphorylation were observed between astrogliaNS and astrogliaST. The rates of l-[U-14C]lactate ([14C]lactate) and d-[U-14C]glucose ([14C]glucose) oxidation were 5.74 ± 0.82 and 2.83 ± 0.4 pmol/60 min/μg protein, respectively, in astrogliaNS grown in low glucose (2 mM) and 3.01 ± 1.03 and 1.77 ± 0.23 pmol/60 min/μg protein, respectively, in astrogliaNS grown in high glucose (22 mM). Neither the [14C]lactate nor the [14C]glucose oxidation rates in astrogliaNS were significantly different from those in astrogliaST. d-aspartate (500 μM) significantly increased the [14C]lactate and [14C]glucose oxidation rates by 127% and 62%, respectively, in astrogliaNS grown in low glucose and by 217% and 115%, respectively, in astrogliaNS grown in high glucose. d-aspartate also increased the oxidation of [14C]lactate and [14C]glucose to 236% and 147% of the control values, respectively, in astrogliaST grown in low glucose and to 174% and 144%, respectively, in astrogliaST grown in high glucose. Rat astroglia differentiated from neurospheres might possess an equivalent capacity for utilizing energy substrates under both basal and activated conditions to that of astroglia prepared from striatum.

AB - The metabolic properties of astroglia differentiated from neurospheres have not been fully assessed. In this study, the glycolytic and oxidative metabolism of glucose in astroglia differentiated from rat tertiary neurospheres (astrogliaNS) was compared with that in astroglia prepared from the striata of embryonic day 16 rats (astrogliaST). In addition to the basal condition, we also investigated energy metabolism under Na+,K+-ATPase activation. Furthermore, the effects of glucose concentration in the culture medium were assessed. No significant differences in 2-deoxy-d-[1-14C]glucose phosphorylation were observed between astrogliaNS and astrogliaST. The rates of l-[U-14C]lactate ([14C]lactate) and d-[U-14C]glucose ([14C]glucose) oxidation were 5.74 ± 0.82 and 2.83 ± 0.4 pmol/60 min/μg protein, respectively, in astrogliaNS grown in low glucose (2 mM) and 3.01 ± 1.03 and 1.77 ± 0.23 pmol/60 min/μg protein, respectively, in astrogliaNS grown in high glucose (22 mM). Neither the [14C]lactate nor the [14C]glucose oxidation rates in astrogliaNS were significantly different from those in astrogliaST. d-aspartate (500 μM) significantly increased the [14C]lactate and [14C]glucose oxidation rates by 127% and 62%, respectively, in astrogliaNS grown in low glucose and by 217% and 115%, respectively, in astrogliaNS grown in high glucose. d-aspartate also increased the oxidation of [14C]lactate and [14C]glucose to 236% and 147% of the control values, respectively, in astrogliaST grown in low glucose and to 174% and 144%, respectively, in astrogliaST grown in high glucose. Rat astroglia differentiated from neurospheres might possess an equivalent capacity for utilizing energy substrates under both basal and activated conditions to that of astroglia prepared from striatum.

KW - Glucose

KW - Lactate

KW - Neural stem cell

UR - http://www.scopus.com/inward/record.url?scp=33745819592&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745819592&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2006.05.009

DO - 10.1016/j.brainres.2006.05.009

M3 - Article

VL - 1101

SP - 5

EP - 11

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -