Metabolome analysis based on capillary electrophoresis-mass spectrometry

Akiyoshi Hirayama, Masataka Wakayama, Tomoyoshi Soga

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a powerful new tool for comprehensive analysis of charged compounds. In this review, we provide a general description of the application of CE-MS in metabolome analysis, including the separation modes of CE, various interfaces and the mass spectrometers used. We also discuss strategies for sample pretreatment, data processing and peak identification, which are important processes in metabolome analysis. In addition, we highlight a number of new techniques to improve metabolite extraction, peak resolution and sensitivity. Finally, we provide some general conclusions and future perspectives.

Original languageEnglish
Pages (from-to)215-222
Number of pages8
JournalTrAC - Trends in Analytical Chemistry
Volume61
DOIs
Publication statusPublished - 2014 Oct 1

Fingerprint

Capillary electrophoresis
Mass spectrometry
electrokinesis
mass spectrometry
Mass spectrometers
Metabolites
metabolite
spectrometer
analysis

Keywords

  • Capillary electrophoresis
  • CE-MS
  • Energy metabolism
  • Mass spectrometry
  • Metabolite identification
  • Metabolome analysis
  • Metabolomics
  • Sample pretreatment
  • Separation
  • Sheathless interface

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Metabolome analysis based on capillary electrophoresis-mass spectrometry. / Hirayama, Akiyoshi; Wakayama, Masataka; Soga, Tomoyoshi.

In: TrAC - Trends in Analytical Chemistry, Vol. 61, 01.10.2014, p. 215-222.

Research output: Contribution to journalArticle

@article{0a73a5a7e4e649bc9e52efd7ea9e1d56,
title = "Metabolome analysis based on capillary electrophoresis-mass spectrometry",
abstract = "Capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a powerful new tool for comprehensive analysis of charged compounds. In this review, we provide a general description of the application of CE-MS in metabolome analysis, including the separation modes of CE, various interfaces and the mass spectrometers used. We also discuss strategies for sample pretreatment, data processing and peak identification, which are important processes in metabolome analysis. In addition, we highlight a number of new techniques to improve metabolite extraction, peak resolution and sensitivity. Finally, we provide some general conclusions and future perspectives.",
keywords = "Capillary electrophoresis, CE-MS, Energy metabolism, Mass spectrometry, Metabolite identification, Metabolome analysis, Metabolomics, Sample pretreatment, Separation, Sheathless interface",
author = "Akiyoshi Hirayama and Masataka Wakayama and Tomoyoshi Soga",
year = "2014",
month = "10",
day = "1",
doi = "10.1016/j.trac.2014.05.005",
language = "English",
volume = "61",
pages = "215--222",
journal = "TrAC - Trends in Analytical Chemistry",
issn = "0165-9936",
publisher = "Elsevier",

}

TY - JOUR

T1 - Metabolome analysis based on capillary electrophoresis-mass spectrometry

AU - Hirayama, Akiyoshi

AU - Wakayama, Masataka

AU - Soga, Tomoyoshi

PY - 2014/10/1

Y1 - 2014/10/1

N2 - Capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a powerful new tool for comprehensive analysis of charged compounds. In this review, we provide a general description of the application of CE-MS in metabolome analysis, including the separation modes of CE, various interfaces and the mass spectrometers used. We also discuss strategies for sample pretreatment, data processing and peak identification, which are important processes in metabolome analysis. In addition, we highlight a number of new techniques to improve metabolite extraction, peak resolution and sensitivity. Finally, we provide some general conclusions and future perspectives.

AB - Capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a powerful new tool for comprehensive analysis of charged compounds. In this review, we provide a general description of the application of CE-MS in metabolome analysis, including the separation modes of CE, various interfaces and the mass spectrometers used. We also discuss strategies for sample pretreatment, data processing and peak identification, which are important processes in metabolome analysis. In addition, we highlight a number of new techniques to improve metabolite extraction, peak resolution and sensitivity. Finally, we provide some general conclusions and future perspectives.

KW - Capillary electrophoresis

KW - CE-MS

KW - Energy metabolism

KW - Mass spectrometry

KW - Metabolite identification

KW - Metabolome analysis

KW - Metabolomics

KW - Sample pretreatment

KW - Separation

KW - Sheathless interface

UR - http://www.scopus.com/inward/record.url?scp=84907016229&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907016229&partnerID=8YFLogxK

U2 - 10.1016/j.trac.2014.05.005

DO - 10.1016/j.trac.2014.05.005

M3 - Article

AN - SCOPUS:84907016229

VL - 61

SP - 215

EP - 222

JO - TrAC - Trends in Analytical Chemistry

JF - TrAC - Trends in Analytical Chemistry

SN - 0165-9936

ER -