Metabolome profiling of various seaweed species discriminates between brown, red, and green algae

Shahlizah Sahul Hamid, Masataka Wakayama, Kensuke Ichihara, Katsutoshi Sakurai, Yujin Ashino, Rie Kadowaki, Tomoyoshi Soga, Masaru Tomita

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Main Conclusion: Among seaweed groups, brown algae had characteristically high concentrations of mannitol, and green algae were characterised by fructose. In red algae, metabolite profiles of individual species should be evaluated. Seaweeds are metabolically different from terrestrial plants. However, general metabolite profiles of the three major seaweed groups, the brown, red, and green algae, and the effect of various extraction methods on metabolite profiling results have not been comprehensively explored. In this study, we evaluated the water-soluble metabolites in four brown, five red, and two green algae species collected from two sites in northern Japan, located in the Sea of Japan and the Pacific Ocean. Freeze-dried seaweed samples were processed by methanol–water extraction with or without chloroform and analysed by capillary electrophoresis- and liquid chromatography-mass spectrometry for metabolite characterisation. The metabolite concentration profiles showed distinctive characteristic depends on species and taxonomic groups, whereas the extraction methods did not have a significant effect. Taxonomic differences between the various seaweed metabolite profiles were well defined using only sugar metabolites but no other major compound types. Mannitol was the main sugar metabolites in brown algae, whereas fructose, sucrose, and glucose were found at high concentrations in green algae. In red algae, individual species had some characteristic metabolites, such as sorbitol in Pyropia pseudolinearis and panose in Dasya sessilis. The metabolite profiles generated in this study will be a resource and provide guidance for nutraceutical research studies because the information about metabolites in seaweeds is still very limited compared to that of terrestrial plants.

Original languageEnglish
JournalPlanta
DOIs
Publication statusPublished - 2019 Jan 1

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Phaeophyta
Rhodophyta
Seaweed
metabolome
Chlorophyta
Phaeophyceae
Metabolome
macroalgae
metabolites
Mannitol
Fructose
Japan
Pacific Ocean
Sorbitol
Capillary Electrophoresis
Chloroform
Dietary Supplements
mannitol
Oceans and Seas
Liquid Chromatography

Keywords

  • Brown algae
  • Extraction methods
  • Green algae
  • Metabolites
  • Red algae

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

Metabolome profiling of various seaweed species discriminates between brown, red, and green algae. / Hamid, Shahlizah Sahul; Wakayama, Masataka; Ichihara, Kensuke; Sakurai, Katsutoshi; Ashino, Yujin; Kadowaki, Rie; Soga, Tomoyoshi; Tomita, Masaru.

In: Planta, 01.01.2019.

Research output: Contribution to journalArticle

Hamid, Shahlizah Sahul ; Wakayama, Masataka ; Ichihara, Kensuke ; Sakurai, Katsutoshi ; Ashino, Yujin ; Kadowaki, Rie ; Soga, Tomoyoshi ; Tomita, Masaru. / Metabolome profiling of various seaweed species discriminates between brown, red, and green algae. In: Planta. 2019.
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