Metabolomic identification of the target of the filopodia protrusion inhibitor glucopiericidin A

Mitsuhiro Kitagawa, Satsuki Ikeda, Etsu Tashiro, Tomoyoshi Soga, Masaya Imoto

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Identifying the targets of bioactive compounds is a major challenge in chemical biological research. Here, we identified the functional target of the natural bioactive compound glucopiericidin A (GPA) through metabolomic analysis. We isolated GPA while screening microbial samples for a filopodia protrusion inhibitor. Interestingly, GPA alone did not inhibit filopodia protrusion, but synergistically inhibit protrusion with the mitochondrial respiration inhibitor, piericidin A (PA). These results suggested that GPA might inhibit glycolysis. Capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) provided strong evidence that GPA suppresses glycolysis by functionally targeting the glucose transporter. GPA may therefore serve as a glucose transporter chemical probe. Simultaneous inhibition of both glycolysis and mitochondrial respiration dramatically decreased intracellular ATP levels, indicating that GPA inhibits ATP-dependent filopodia protrusion with PA. Our results represent a challenge of molecular target identification using metabolomic analysis.

Original languageEnglish
Pages (from-to)989-998
Number of pages10
JournalChemistry and Biology
Volume17
Issue number9
DOIs
Publication statusPublished - 2010 Sep 24

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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