Metabolomic profiling of anionic metabolites by capillary electrophoresis mass spectrometry

Tomoyoshi Soga, Kaori Igarashi, Chiharu Ito, Katsuo Mizobuchi, Hans Peter Zimmermann, Masaru Tomita

Research output: Contribution to journalArticle

181 Citations (Scopus)

Abstract

We describe a sheath flow capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) method in the negative mode using a platinum electrospray ionization (ESI) spray needle, which allows the comprehensive analysis of anionic metabolites. The material of the spray needle had significant effect on the measurement of anions. A stainless steel spray needle was oxidized and corroded at the anodic electrode due to electrolysis. The precipitation of iron oxides (rust) plugged the capillary outlet, resulting in shortened capillary lifetime. Many anionic metabolites also formed complexes with the iron oxides or migrating nickel ion, which was also generated by electrolysis and moved toward the cathode (the capillary inlet). The metal-anion complex formation significantly reduced detection sensitivity of the anionic compounds. The use of a platinum ESI needle prevented both oxidation of the metals and needle corrosion. Sensitivity using the platinum needle increased from several- to 63-fold, with the largest improvements for anions exhibiting high metal chelating properties such as carboxylic acids, nucleotides, and coenzyme A compounds. The detection limits for most anions were between 0.03 and 0.87 μmol/L (0.8 and 24 fmol) at a signal-to-noise ratio of 3. This method is quantitative, sensitive, and robust, and its utility was demonstrated by the analysis of the metabolites in the central metabolic pathways extracted from mouse liver.

Original languageEnglish
Pages (from-to)6165-6174
Number of pages10
JournalAnalytical Chemistry
Volume81
Issue number15
DOIs
Publication statusPublished - 2009 Aug 1

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Capillary electrophoresis
Metabolites
Needles
Mass spectrometry
Anions
Platinum
Electrospray ionization
Metals
Electrolysis
Stainless Steel
Coenzyme A
Carboxylic Acids
Chelation
Nickel
Liver
Metabolomics
Signal to noise ratio
Cathodes
Nucleotides
Ions

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Metabolomic profiling of anionic metabolites by capillary electrophoresis mass spectrometry. / Soga, Tomoyoshi; Igarashi, Kaori; Ito, Chiharu; Mizobuchi, Katsuo; Zimmermann, Hans Peter; Tomita, Masaru.

In: Analytical Chemistry, Vol. 81, No. 15, 01.08.2009, p. 6165-6174.

Research output: Contribution to journalArticle

Soga, Tomoyoshi ; Igarashi, Kaori ; Ito, Chiharu ; Mizobuchi, Katsuo ; Zimmermann, Hans Peter ; Tomita, Masaru. / Metabolomic profiling of anionic metabolites by capillary electrophoresis mass spectrometry. In: Analytical Chemistry. 2009 ; Vol. 81, No. 15. pp. 6165-6174.
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