Metabolomic study of Chilean biomining bacteria Acidithiobacillus ferrooxidans strain Wenelen and Acidithiobacillus thiooxidans strain Licanantay

Patricio Martínez, Sebastián Gálvez, Norimasa Ohtsuka, Marko Budinich, María Paz Cortés, Cristián Serpell, Kenji Nakahigashi, Akiyoshi Hirayama, Masaru Tomita, Tomoyoshi Soga, Servet Martínez, Alejandro Maass, Pilar Parada

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In this study, we present the first metabolic profiles for two bioleaching bacteria using capillary electrophoresis coupled with mass spectrometry. The bacteria, Acidithiobacillus ferrooxidans strain Wenelen (DSM 16786) and Acidithiobacillus thiooxidans strain Licanantay (DSM 17318), were sampled at different growth phases and on different substrates: the former was grown with iron and sulfur, and the latter with sulfur and chalcopyrite. Metabolic profiles were scored from planktonic and sessile states. Spermidine was detected in intra- and extracellular samples for both strains, suggesting it has an important role in biofilm formation in the presence of solid substrate. The canonical pathway for spermidine synthesis seems absent as its upstream precursor, putrescine, was not present in samples. Glutathione, a catalytic activator of elemental sulfur, was identified as one of the most abundant metabolites in the intracellular space in A. thiooxidans strain Licanantay, confirming its participation in the sulfur oxidation pathway. Amino acid profiles varied according to the growth conditions and bioleaching species. Glutamic and aspartic acid were highly abundant in intra- and extracellular extracts. Both are constituents of the extracellular matrix, and have a probable role in cell detoxification. This novel metabolomic information validates previous knowledge from in silico metabolic reconstructions based on genomic sequences, and reveals important biomining functions such as biofilm formation, energy management and stress responses.

Original languageEnglish
Pages (from-to)247-257
Number of pages11
JournalMetabolomics
Volume9
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

Acidithiobacillus thiooxidans
Acidithiobacillus
Metabolomics
Sulfur
Bacteria
Bioleaching
Spermidine
Metabolome
Biofilms
Intracellular Space
Capillary electrophoresis
Detoxification
Putrescine
Energy management
Capillary Electrophoresis
Substrates
Growth
Metabolites
Aspartic Acid
Computer Simulation

Keywords

  • Acidithiobacillus
  • Bioleaching
  • Biomarker
  • Capillary electrophoresis
  • CE-MS
  • Ferrooxidans
  • Licanantay
  • Mass spectrometry
  • Metabolomics
  • Spermidine
  • Thiooxidans
  • Wenelen

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Metabolomic study of Chilean biomining bacteria Acidithiobacillus ferrooxidans strain Wenelen and Acidithiobacillus thiooxidans strain Licanantay. / Martínez, Patricio; Gálvez, Sebastián; Ohtsuka, Norimasa; Budinich, Marko; Cortés, María Paz; Serpell, Cristián; Nakahigashi, Kenji; Hirayama, Akiyoshi; Tomita, Masaru; Soga, Tomoyoshi; Martínez, Servet; Maass, Alejandro; Parada, Pilar.

In: Metabolomics, Vol. 9, No. 1, 2013, p. 247-257.

Research output: Contribution to journalArticle

Martínez, P, Gálvez, S, Ohtsuka, N, Budinich, M, Cortés, MP, Serpell, C, Nakahigashi, K, Hirayama, A, Tomita, M, Soga, T, Martínez, S, Maass, A & Parada, P 2013, 'Metabolomic study of Chilean biomining bacteria Acidithiobacillus ferrooxidans strain Wenelen and Acidithiobacillus thiooxidans strain Licanantay', Metabolomics, vol. 9, no. 1, pp. 247-257. https://doi.org/10.1007/s11306-012-0443-3
Martínez, Patricio ; Gálvez, Sebastián ; Ohtsuka, Norimasa ; Budinich, Marko ; Cortés, María Paz ; Serpell, Cristián ; Nakahigashi, Kenji ; Hirayama, Akiyoshi ; Tomita, Masaru ; Soga, Tomoyoshi ; Martínez, Servet ; Maass, Alejandro ; Parada, Pilar. / Metabolomic study of Chilean biomining bacteria Acidithiobacillus ferrooxidans strain Wenelen and Acidithiobacillus thiooxidans strain Licanantay. In: Metabolomics. 2013 ; Vol. 9, No. 1. pp. 247-257.
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abstract = "In this study, we present the first metabolic profiles for two bioleaching bacteria using capillary electrophoresis coupled with mass spectrometry. The bacteria, Acidithiobacillus ferrooxidans strain Wenelen (DSM 16786) and Acidithiobacillus thiooxidans strain Licanantay (DSM 17318), were sampled at different growth phases and on different substrates: the former was grown with iron and sulfur, and the latter with sulfur and chalcopyrite. Metabolic profiles were scored from planktonic and sessile states. Spermidine was detected in intra- and extracellular samples for both strains, suggesting it has an important role in biofilm formation in the presence of solid substrate. The canonical pathway for spermidine synthesis seems absent as its upstream precursor, putrescine, was not present in samples. Glutathione, a catalytic activator of elemental sulfur, was identified as one of the most abundant metabolites in the intracellular space in A. thiooxidans strain Licanantay, confirming its participation in the sulfur oxidation pathway. Amino acid profiles varied according to the growth conditions and bioleaching species. Glutamic and aspartic acid were highly abundant in intra- and extracellular extracts. Both are constituents of the extracellular matrix, and have a probable role in cell detoxification. This novel metabolomic information validates previous knowledge from in silico metabolic reconstructions based on genomic sequences, and reveals important biomining functions such as biofilm formation, energy management and stress responses.",
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AU - Gálvez, Sebastián

AU - Ohtsuka, Norimasa

AU - Budinich, Marko

AU - Cortés, María Paz

AU - Serpell, Cristián

AU - Nakahigashi, Kenji

AU - Hirayama, Akiyoshi

AU - Tomita, Masaru

AU - Soga, Tomoyoshi

AU - Martínez, Servet

AU - Maass, Alejandro

AU - Parada, Pilar

PY - 2013

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N2 - In this study, we present the first metabolic profiles for two bioleaching bacteria using capillary electrophoresis coupled with mass spectrometry. The bacteria, Acidithiobacillus ferrooxidans strain Wenelen (DSM 16786) and Acidithiobacillus thiooxidans strain Licanantay (DSM 17318), were sampled at different growth phases and on different substrates: the former was grown with iron and sulfur, and the latter with sulfur and chalcopyrite. Metabolic profiles were scored from planktonic and sessile states. Spermidine was detected in intra- and extracellular samples for both strains, suggesting it has an important role in biofilm formation in the presence of solid substrate. The canonical pathway for spermidine synthesis seems absent as its upstream precursor, putrescine, was not present in samples. Glutathione, a catalytic activator of elemental sulfur, was identified as one of the most abundant metabolites in the intracellular space in A. thiooxidans strain Licanantay, confirming its participation in the sulfur oxidation pathway. Amino acid profiles varied according to the growth conditions and bioleaching species. Glutamic and aspartic acid were highly abundant in intra- and extracellular extracts. Both are constituents of the extracellular matrix, and have a probable role in cell detoxification. This novel metabolomic information validates previous knowledge from in silico metabolic reconstructions based on genomic sequences, and reveals important biomining functions such as biofilm formation, energy management and stress responses.

AB - In this study, we present the first metabolic profiles for two bioleaching bacteria using capillary electrophoresis coupled with mass spectrometry. The bacteria, Acidithiobacillus ferrooxidans strain Wenelen (DSM 16786) and Acidithiobacillus thiooxidans strain Licanantay (DSM 17318), were sampled at different growth phases and on different substrates: the former was grown with iron and sulfur, and the latter with sulfur and chalcopyrite. Metabolic profiles were scored from planktonic and sessile states. Spermidine was detected in intra- and extracellular samples for both strains, suggesting it has an important role in biofilm formation in the presence of solid substrate. The canonical pathway for spermidine synthesis seems absent as its upstream precursor, putrescine, was not present in samples. Glutathione, a catalytic activator of elemental sulfur, was identified as one of the most abundant metabolites in the intracellular space in A. thiooxidans strain Licanantay, confirming its participation in the sulfur oxidation pathway. Amino acid profiles varied according to the growth conditions and bioleaching species. Glutamic and aspartic acid were highly abundant in intra- and extracellular extracts. Both are constituents of the extracellular matrix, and have a probable role in cell detoxification. This novel metabolomic information validates previous knowledge from in silico metabolic reconstructions based on genomic sequences, and reveals important biomining functions such as biofilm formation, energy management and stress responses.

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