Metalloprotease-disintegrin ADAM8: Expression analysis and targeted deletion in mice

Kristine Kelly, Gillian Hutchinson, Daniela Nebenius-Oosthuizen, Andrew J H Smith, Jörg W. Bartsch, Keisuke Horiuchi, Andrea Rittger, Katia Manova, Andy J P Docherty, Carl P. Blobel

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

ADAM8 (a disintegrin and metalloprotease 8, also referred to as MS2/CD156a) is a membrane-anchored metalloprotease that was first identified in a macrophage cell line and has been implicated in neurodegenerative diseases. Here, we evaluated the expression of ADAM8 during mouse development and generated mice lacking ADAM8 (Adam8-/- mice). During early mouse development, ADAM8 is expressed by maternal cells in the decidua and by trophoblast derivatives of the embryo but not in the derivatives of the inner cell mass. At later stages, prominent expression of ADAM8 is seen in the embryo proper, in the gonadal ridge, thymus, developing cartilage and bone, brain and spinal cord, and in the mesenchyme in close proximity to the branch point between the jugular vein and developing lymphatic vessels. Examination of Adam8-/- mice, however, revealed no major defects in these or other structures during development or in adult tissues and no evident pathological phenotypes.

Original languageEnglish
Pages (from-to)221-231
Number of pages11
JournalDevelopmental Dynamics
Volume232
Issue number1
DOIs
Publication statusPublished - 2005 Jan
Externally publishedYes

Fingerprint

Disintegrins
Metalloproteases
Embryonic Structures
Decidua
Lymphatic Vessels
Jugular Veins
Trophoblasts
Mesoderm
Neurodegenerative Diseases
Thymus Gland
Cartilage
Spinal Cord
Macrophages
Mothers
Phenotype
Bone and Bones
Cell Line
Membranes
Brain

Keywords

  • ADAM
  • CD156a
  • Lymphatic development
  • Metalloprotease-disintegrin
  • MS2

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Kelly, K., Hutchinson, G., Nebenius-Oosthuizen, D., Smith, A. J. H., Bartsch, J. W., Horiuchi, K., ... Blobel, C. P. (2005). Metalloprotease-disintegrin ADAM8: Expression analysis and targeted deletion in mice. Developmental Dynamics, 232(1), 221-231. https://doi.org/10.1002/dvdy.20221

Metalloprotease-disintegrin ADAM8 : Expression analysis and targeted deletion in mice. / Kelly, Kristine; Hutchinson, Gillian; Nebenius-Oosthuizen, Daniela; Smith, Andrew J H; Bartsch, Jörg W.; Horiuchi, Keisuke; Rittger, Andrea; Manova, Katia; Docherty, Andy J P; Blobel, Carl P.

In: Developmental Dynamics, Vol. 232, No. 1, 01.2005, p. 221-231.

Research output: Contribution to journalArticle

Kelly, K, Hutchinson, G, Nebenius-Oosthuizen, D, Smith, AJH, Bartsch, JW, Horiuchi, K, Rittger, A, Manova, K, Docherty, AJP & Blobel, CP 2005, 'Metalloprotease-disintegrin ADAM8: Expression analysis and targeted deletion in mice', Developmental Dynamics, vol. 232, no. 1, pp. 221-231. https://doi.org/10.1002/dvdy.20221
Kelly K, Hutchinson G, Nebenius-Oosthuizen D, Smith AJH, Bartsch JW, Horiuchi K et al. Metalloprotease-disintegrin ADAM8: Expression analysis and targeted deletion in mice. Developmental Dynamics. 2005 Jan;232(1):221-231. https://doi.org/10.1002/dvdy.20221
Kelly, Kristine ; Hutchinson, Gillian ; Nebenius-Oosthuizen, Daniela ; Smith, Andrew J H ; Bartsch, Jörg W. ; Horiuchi, Keisuke ; Rittger, Andrea ; Manova, Katia ; Docherty, Andy J P ; Blobel, Carl P. / Metalloprotease-disintegrin ADAM8 : Expression analysis and targeted deletion in mice. In: Developmental Dynamics. 2005 ; Vol. 232, No. 1. pp. 221-231.
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