Metalloprotease-disintegrin ADAM8: Expression analysis and targeted deletion in mice

Kristine Kelly, Gillian Hutchinson, Daniela Nebenius-Oosthuizen, Andrew J.H. Smith, Jörg W. Bartsch, Keisuke Horiuchi, Andrea Rittger, Katia Manova, Andy J.P. Docherty, Carl P. Blobel

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Abstract

ADAM8 (a disintegrin and metalloprotease 8, also referred to as MS2/CD156a) is a membrane-anchored metalloprotease that was first identified in a macrophage cell line and has been implicated in neurodegenerative diseases. Here, we evaluated the expression of ADAM8 during mouse development and generated mice lacking ADAM8 (Adam8-/- mice). During early mouse development, ADAM8 is expressed by maternal cells in the decidua and by trophoblast derivatives of the embryo but not in the derivatives of the inner cell mass. At later stages, prominent expression of ADAM8 is seen in the embryo proper, in the gonadal ridge, thymus, developing cartilage and bone, brain and spinal cord, and in the mesenchyme in close proximity to the branch point between the jugular vein and developing lymphatic vessels. Examination of Adam8-/- mice, however, revealed no major defects in these or other structures during development or in adult tissues and no evident pathological phenotypes.

Original languageEnglish
Pages (from-to)221-231
Number of pages11
JournalDevelopmental Dynamics
Volume232
Issue number1
DOIs
Publication statusPublished - 2005 Jan 1

Keywords

  • ADAM
  • CD156a
  • Lymphatic development
  • MS2
  • Metalloprotease-disintegrin

ASJC Scopus subject areas

  • Developmental Biology

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    Kelly, K., Hutchinson, G., Nebenius-Oosthuizen, D., Smith, A. J. H., Bartsch, J. W., Horiuchi, K., Rittger, A., Manova, K., Docherty, A. J. P., & Blobel, C. P. (2005). Metalloprotease-disintegrin ADAM8: Expression analysis and targeted deletion in mice. Developmental Dynamics, 232(1), 221-231. https://doi.org/10.1002/dvdy.20221