Method of localized removal of cells using a bolt-clamped Langevin transducer with an ultrasonic horn

Takumi Inui, Yuta Kurashina, Chikahiro Imashiro, Kenjiro Takemura

Research output: Contribution to journalArticle

Abstract

Cell isolation by eliminating undesirable cell aggregations or colonies with low activity is essential to improve cell culture efficiency. Moreover, when creating tissues from induced pluripotent stem cells, residual undifferentiated cells must be removed to prevent tumor formation in vivo. Here, we evaluated the use of ultrasonic irradiation, which can apply energy locally without contact, and proposed a method to eliminate cells in a small area of culture by ultrasonic irradiation from a Langevin transducer. We constructed a device that incorporated a bolt-clamped 19.84 kHz Langevin transducer with an ultrasonic horn and determined the optimal conditions for stable elimination of cells in small areas of a 35-mm culture dish. The optimal conditions were as follows: number of cycles = 400, clearance distance = 1 mm, volume of medium = 4 mL, and distance from the center of culture surface = 0 mm. The mean cell elimination area under these conditions was 0.097 mm2. We also evaluated the viability of neighboring cells after ultrasonic irradiation by fluorescent staining and found that most cells around the elimination area survived. These findings suggest that the proposed method has potential for localized elimination of cells without the need for contact with the cell surface.

Original languageEnglish
JournalEngineering in Life Sciences
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Bolts
Horns
Transducers
Ultrasonics
Irradiation
Stem cells
Cell culture
Tumors
Agglomeration
Cells
Induced Pluripotent Stem Cells
Cell Aggregation
Tissue
Cell Separation
Cell Survival
Cell Culture Techniques
Staining and Labeling
Equipment and Supplies
Neoplasms

Keywords

  • cell culture efficiency
  • cell elimination
  • induced pluripotent stem cells
  • Langevin transducer
  • ultrasonic waves

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Engineering
  • Bioengineering

Cite this

Method of localized removal of cells using a bolt-clamped Langevin transducer with an ultrasonic horn. / Inui, Takumi; Kurashina, Yuta; Imashiro, Chikahiro; Takemura, Kenjiro.

In: Engineering in Life Sciences, 01.01.2019.

Research output: Contribution to journalArticle

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