Abstract
Microscopic imaging techniques have been developed to visualize events occurring in biological cells. Coherent X-ray diffraction imaging is one of the techniques applicable to structural analyses of cells and organelles, which have never been crystallized. In the experiment, a single noncrystalline particle is illuminated by an X-ray beam with almost complete spatial coherence. The structure of the particle projected along the direction of the beam is, in principle, retrieved from a finely recorded diffraction pattern alone by using iterative phase-retrieval algorithms. Here, we describe fundamental theory and experimental methods of coherent X-ray diffraction imaging and the recent application in structural studies of noncrystalline specimens by using X-rays available at Super Photon Ring of 8-Gev and SPring-8 Angstrom Compact Free Electron Laser in Japan.
Original language | English |
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Pages (from-to) | 541-567 |
Number of pages | 27 |
Journal | Biophysical Reviews |
Volume | 12 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2020 Apr 1 |
Keywords
- Coherent X-ray diffraction imaging
- Cryogenic experiment
- Noncrystalline particle
- Synchrotron radiation
- Three-dimensional structure
- X-ray free electron laser
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Molecular Biology