TY - JOUR
T1 - Methods and application of coherent X-ray diffraction imaging of noncrystalline particles
AU - Nakasako, Masayoshi
AU - Kobayashi, Amane
AU - Takayama, Yuki
AU - Asakura, Kenta
AU - Oide, Mao
AU - Okajima, Koji
AU - Oroguchi, Tomotaka
AU - Yamamoto, Masaki
N1 - Funding Information:
The construction and development of the two diffraction apparatus was supported by a grant for X-ray Free Electron Laser Key Technology and the X-ray Free Electron Laser Priority Strategy Program from the Ministry of Education, Culture, Sports, Science and Technology, Japan. In addition, the development of specimen preparation techniques and data analysis software was supported by grants to the author from the Japan Science Promotion Society (Nos. jp11558086, jp17654084, jp1920402, jp22244054, jp24654140, jp16H02218) and by grants to the author from the Ministry of Education, Culture, Sports, Science and Technology, Japan (Nos. jp10157202, jp15076210, jp23120525, jp25120725, jp17H05891). The cXDI experiments at BL29XUL of SPring-8 were performed at the RIKEN beamline of SPring-8 (proposal Nos. 20090097, 20100035, 20110006, 20140096, 20150098, 20160084, 20170017, 20180070, and 20190005). The XFEL-cXDI experiments were carried out at BL3 of SACLA (proposal Nos. 2012A8005, 2012B8037, 2013A8043, 2013B8049, 2014A8033, 2014B8052, 2015A8051, 2015B8049, 2016A8048, 2016B8064, 2017A8015, and 2017B8003). The PR calculations and multivariate analyses were performed using the mini-K supercomputer system at the SACLA facility. Acknowledgments
Publisher Copyright:
© 2020, International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Microscopic imaging techniques have been developed to visualize events occurring in biological cells. Coherent X-ray diffraction imaging is one of the techniques applicable to structural analyses of cells and organelles, which have never been crystallized. In the experiment, a single noncrystalline particle is illuminated by an X-ray beam with almost complete spatial coherence. The structure of the particle projected along the direction of the beam is, in principle, retrieved from a finely recorded diffraction pattern alone by using iterative phase-retrieval algorithms. Here, we describe fundamental theory and experimental methods of coherent X-ray diffraction imaging and the recent application in structural studies of noncrystalline specimens by using X-rays available at Super Photon Ring of 8-Gev and SPring-8 Angstrom Compact Free Electron Laser in Japan.
AB - Microscopic imaging techniques have been developed to visualize events occurring in biological cells. Coherent X-ray diffraction imaging is one of the techniques applicable to structural analyses of cells and organelles, which have never been crystallized. In the experiment, a single noncrystalline particle is illuminated by an X-ray beam with almost complete spatial coherence. The structure of the particle projected along the direction of the beam is, in principle, retrieved from a finely recorded diffraction pattern alone by using iterative phase-retrieval algorithms. Here, we describe fundamental theory and experimental methods of coherent X-ray diffraction imaging and the recent application in structural studies of noncrystalline specimens by using X-rays available at Super Photon Ring of 8-Gev and SPring-8 Angstrom Compact Free Electron Laser in Japan.
KW - Coherent X-ray diffraction imaging
KW - Cryogenic experiment
KW - Noncrystalline particle
KW - Synchrotron radiation
KW - Three-dimensional structure
KW - X-ray free electron laser
UR - http://www.scopus.com/inward/record.url?scp=85082778932&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85082778932&partnerID=8YFLogxK
U2 - 10.1007/s12551-020-00690-9
DO - 10.1007/s12551-020-00690-9
M3 - Review article
AN - SCOPUS:85082778932
VL - 12
SP - 541
EP - 567
JO - Biophysical Reviews
JF - Biophysical Reviews
SN - 1867-2450
IS - 2
ER -