Methylation status of ribosomal RNA gene clusters in the flow-sorted human acrocentric chromosomes

Kazuhiko Kawasaki, Shinsei Minoshima, Jun Kudo, Ryuichi Fukuyama, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

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Abstract

Southern blot analysis of the human acrocentric chromosomes that were flow-sorted from B-lymphoblastoid cell line GM130B revealed that the sensitivity of the ribosomal RNA (rDNA) gene clusters to the restriction enzyme NotI differs among these rDNA-containing chromosomes: the rDNA clusters of Chromosomes (Chr) 13, 14, and 15 are much more sensitive to NotI digestion than those of Chrs 21 and 22 in this particular cell line. Detailed analysis by use of methylation-sensitive enzymes HpaII and HhaI and methylation-insensitive enzyme MspI confirmed the significant variation in the methylation status of rDNA clusters among these chromosomes. Quantitative analysis by fluorescent in situ hybridization (FISH) indicated that copy number of rDNA varies among individual chromosomes, but the average copy number in the acrocentric Chrs 21 and 22 is significantly greater than that of the Chrs 13, 14, and 15 in GM130B cells. Similar analysis reveals that the methylation status of rDNA clusters in another B-lymphoblastoid cell line GM131 was different from that of GM130B. These data together indicate that the copy number and methylation patterns of rDNA clusters differ among individual acrocentric chromosomes in a given cell line, and they are different among cell lines.

Original languageEnglish
Pages (from-to)173-178
Number of pages6
JournalMammalian Genome
Volume3
Issue number3
DOIs
Publication statusPublished - 1992 Mar

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Human Chromosomes
Multigene Family
Ribosomal DNA
Methylation
Cell Line
Chromosomes
Enzymes
Chromosomes, Human, Pair 15
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 13
Southern Blotting
Fluorescence In Situ Hybridization
Digestion

ASJC Scopus subject areas

  • Genetics

Cite this

Methylation status of ribosomal RNA gene clusters in the flow-sorted human acrocentric chromosomes. / Kawasaki, Kazuhiko; Minoshima, Shinsei; Kudo, Jun; Fukuyama, Ryuichi; Shimizu, Nobuyoshi.

In: Mammalian Genome, Vol. 3, No. 3, 03.1992, p. 173-178.

Research output: Contribution to journalArticle

Kawasaki, K, Minoshima, S, Kudo, J, Fukuyama, R & Shimizu, N 1992, 'Methylation status of ribosomal RNA gene clusters in the flow-sorted human acrocentric chromosomes', Mammalian Genome, vol. 3, no. 3, pp. 173-178. https://doi.org/10.1007/BF00352463
Kawasaki, Kazuhiko ; Minoshima, Shinsei ; Kudo, Jun ; Fukuyama, Ryuichi ; Shimizu, Nobuyoshi. / Methylation status of ribosomal RNA gene clusters in the flow-sorted human acrocentric chromosomes. In: Mammalian Genome. 1992 ; Vol. 3, No. 3. pp. 173-178.
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