Metre-long cell-laden microfibres exhibit tissue morphologies and functions

Hiroaki Onoe, Teru Okitsu, Akane Itou, Midori Kato-Negishi, Riho Gojo, Daisuke Kiriya, Koji Sato, Shigenori Miura, Shintaroh Iwanaga, Kaori Kuribayashi-Shigetomi, Yukiko T. Matsunaga, Yuto Shimoyama, Shoji Takeuchi

Research output: Contribution to journalArticle

410 Citations (Scopus)

Abstract

Artificial reconstruction of fibre-shaped cellular constructs could greatly contribute to tissue assembly in vitro. Here we show that, by using a microfluidic device with double-coaxial laminar flow, metre-long core-shell hydrogel microfibres encapsulating ECM proteins and differentiated cells or somatic stem cells can be fabricated, and that the microfibres reconstitute intrinsic morphologies and functions of living tissues. We also show that these functional fibres can be assembled, by weaving and reeling, into macroscopic cellular structures with various spatial patterns. Moreover, fibres encapsulating primary pancreatic islet cells and transplanted through a microcatheter into the subrenal capsular space of diabetic mice normalized blood glucose concentrations for about two weeks. These microfibres may find use as templates for the reconstruction of fibre-shaped functional tissues that mimic muscle fibres, blood vessels or nerve networks in vivo.

Original languageEnglish
Pages (from-to)584-590
Number of pages7
JournalNature Materials
Volume12
Issue number6
DOIs
Publication statusPublished - 2013 Jun
Externally publishedYes

Fingerprint

microfibers
Tissue
encapsulating
fibers
Fibers
cells
weaving
muscle fibers
stem cells
microfluidic devices
blood vessels
nerves
Military electronic countermeasures
laminar flow
glucose
Hydrogel
blood
mice
Blood vessels
Stem cells

ASJC Scopus subject areas

  • Mechanical Engineering
  • Mechanics of Materials
  • Condensed Matter Physics
  • Materials Science(all)
  • Chemistry(all)

Cite this

Onoe, H., Okitsu, T., Itou, A., Kato-Negishi, M., Gojo, R., Kiriya, D., ... Takeuchi, S. (2013). Metre-long cell-laden microfibres exhibit tissue morphologies and functions. Nature Materials, 12(6), 584-590. https://doi.org/10.1038/nmat3606

Metre-long cell-laden microfibres exhibit tissue morphologies and functions. / Onoe, Hiroaki; Okitsu, Teru; Itou, Akane; Kato-Negishi, Midori; Gojo, Riho; Kiriya, Daisuke; Sato, Koji; Miura, Shigenori; Iwanaga, Shintaroh; Kuribayashi-Shigetomi, Kaori; Matsunaga, Yukiko T.; Shimoyama, Yuto; Takeuchi, Shoji.

In: Nature Materials, Vol. 12, No. 6, 06.2013, p. 584-590.

Research output: Contribution to journalArticle

Onoe, H, Okitsu, T, Itou, A, Kato-Negishi, M, Gojo, R, Kiriya, D, Sato, K, Miura, S, Iwanaga, S, Kuribayashi-Shigetomi, K, Matsunaga, YT, Shimoyama, Y & Takeuchi, S 2013, 'Metre-long cell-laden microfibres exhibit tissue morphologies and functions', Nature Materials, vol. 12, no. 6, pp. 584-590. https://doi.org/10.1038/nmat3606
Onoe H, Okitsu T, Itou A, Kato-Negishi M, Gojo R, Kiriya D et al. Metre-long cell-laden microfibres exhibit tissue morphologies and functions. Nature Materials. 2013 Jun;12(6):584-590. https://doi.org/10.1038/nmat3606
Onoe, Hiroaki ; Okitsu, Teru ; Itou, Akane ; Kato-Negishi, Midori ; Gojo, Riho ; Kiriya, Daisuke ; Sato, Koji ; Miura, Shigenori ; Iwanaga, Shintaroh ; Kuribayashi-Shigetomi, Kaori ; Matsunaga, Yukiko T. ; Shimoyama, Yuto ; Takeuchi, Shoji. / Metre-long cell-laden microfibres exhibit tissue morphologies and functions. In: Nature Materials. 2013 ; Vol. 12, No. 6. pp. 584-590.
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