Micro-rotation flow chamber rapidly forming collagen gel-mediated hetero-spheroids

Hiroki Ota, Taiga Kodama, Masayuki Yamato, Teruo Okano, Norihisa Miki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Spheroids that are formed from aggregated cells enhance biological function compared to monolayer culture. In particular, hetero-spheroids composed of different types of cells, such as hepatocytes and endothelial cells, express tissue specific functions at a high level, which is advantageous for more precise drug screening and biological research. In this study, we propose rapid formation of three-dimensional hetero-spheroids consisting of hepatocytes and endothelial cells using micro-rotation flow. The hepatocytes are coated with collagen gel layers less than 200 nm thick to increase adhesion strength between hepatocytes and endothelial cells. Gel-coated hepatocytes and endothelial cells are collected in an array by micro-rotational flow and collagen-gel coating, thereby forming hetero-spheroids within 2 min. This array allowed the size of the three-dimensional spheroids to be hydrodynamically controlled by varying the cell density of the medium without altering the device geometry with standard deviations of less than 19%. The proposed microfulidic device, with its capacity of rapidly forming size-controlled hetero-cell aggregates, will offer an efficient experimental platform for heterospheroid study that will contribute to drug screening and regenerative medicine.

Original languageEnglish
Title of host publication2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
Pages94-98
Number of pages5
DOIs
Publication statusPublished - 2012
Event22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation - Nagoya, Japan
Duration: 2011 Nov 62011 Nov 9

Other

Other22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation
CountryJapan
CityNagoya
Period11/11/611/11/9

Fingerprint

Collagen
Endothelial cells
Gels
Screening
Rotational flow
Bond strength (materials)
Monolayers
Hepatocytes
Tissue
Coatings
Geometry

ASJC Scopus subject areas

  • Artificial Intelligence
  • Mechanical Engineering

Cite this

Ota, H., Kodama, T., Yamato, M., Okano, T., & Miki, N. (2012). Micro-rotation flow chamber rapidly forming collagen gel-mediated hetero-spheroids. In 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation" (pp. 94-98). [6102166] https://doi.org/10.1109/MHS.2011.6102166

Micro-rotation flow chamber rapidly forming collagen gel-mediated hetero-spheroids. / Ota, Hiroki; Kodama, Taiga; Yamato, Masayuki; Okano, Teruo; Miki, Norihisa.

2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation". 2012. p. 94-98 6102166.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ota, H, Kodama, T, Yamato, M, Okano, T & Miki, N 2012, Micro-rotation flow chamber rapidly forming collagen gel-mediated hetero-spheroids. in 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"., 6102166, pp. 94-98, 22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation, Nagoya, Japan, 11/11/6. https://doi.org/10.1109/MHS.2011.6102166
Ota H, Kodama T, Yamato M, Okano T, Miki N. Micro-rotation flow chamber rapidly forming collagen gel-mediated hetero-spheroids. In 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation". 2012. p. 94-98. 6102166 https://doi.org/10.1109/MHS.2011.6102166
Ota, Hiroki ; Kodama, Taiga ; Yamato, Masayuki ; Okano, Teruo ; Miki, Norihisa. / Micro-rotation flow chamber rapidly forming collagen gel-mediated hetero-spheroids. 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation". 2012. pp. 94-98
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